Effect of two intermediate electron donors, NADPH and FADH2, on Spirulina Δ6-desaturase co-expressed with two different immediate electron donors, cytochrome b5 and ferredoxin, in Escherichia coli

Molecular Biology Reports

Volumn 34, Issue 4, 2007, Pages 261-266

  Kurdrid, Pavinee ^a   Subudhi, Sanjukta ^a   Cheevadhanarak, Supapon ^a   Tanticharoen, Morakot ^a   Hongsthong, Apiradee ^a  

^a KING MONGKUT'S UNIVERSITY OF TECHNOLOGY THONBURI   (Thailand)

Author keywords

Coexpression; Delta 6 desaturase; Electron donor; Spirulina platensis

Indexed keywords

CYTOCHROME B5; FERREDOXIN; FLAVINE ADENINE NUCLEOTIDE; GAMMA LINOLENIC ACID; LINOLEOYL COENZYME A DESATURASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

ARTICLE; CATALYSIS; ELECTRON; ESCHERICHIA COLI; GENE EXPRESSION; HOST CELL; MUCOR; MUCOR ROUXII; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN FUNCTION; SPIRULINA; SPIRULINA PLATENSIS;

BASE SEQUENCE; BLOTTING, WESTERN; CHROMATOGRAPHY, GAS; CYTOCHROMES B; ESCHERICHIA COLI; FATTY ACIDS; FERREDOXINS; FLAVIN-ADENINE DINUCLEOTIDE; GENE EXPRESSION REGULATION, BACTERIAL; LINOLEOYL-COA DESATURASE; MOLECULAR SEQUENCE DATA; NADP; RECOMBINANT PROTEINS; SPIRULINA;

AMYLOMYCES ROUXII; ESCHERICHIA COLI; SPIRULINA; SPIRULINA PLATENSIS;

EID: 35448976036     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-006-9040-8     Document Type: Article

References (19)

1. Cahoon E, Mills LA, Shanklin J (1996) Modification of the fatty acid composition of Escherichia coli by coexpression of a plant acyl-acyl carrier protein desaturase and feredoxin. J Bacteriol 178:936-939
2. Gravel P, Golaz O (1996) In: Walker JM (ed) Humana Press, New Jersy, pp 249-260
3. Hongsthong A, Deshnium P, Paithoonrangsarid K, Cheevadhanarak S, Tanticharoen M (2003) Differential responses of the three acyl-lipid desaturases to immediate temperature reduction occurred in two lipid membranes of Spirulina platensis strain C1. J Biosci Bioeng 96:519-524
4. Hongsthong A, Paithoonrangsarid K, Phapugrankul P, Sirijuntarut M, Cheevadhanarak S, Tanticharoen M (2004) The expression of three desaturase genes of Spirulina platensis in Escherichia coli DH5α. Mol Biol Reports 31:177-189
5. 5 domain and Spirulina- 6-acyl-lipid desaturase. Appl Microbiol Biotechnol (in press)
6. + oxidoreductase from Escherichia coli. FEBS Lett 529:237-242
7. Kurdrid P, Subudhi S, Hongsthong A, Ruengjitchatchawalya M, Tanticharoen M (2005) Functional expression of Spirulina-delta6 desaturase gene in yeast, Saccharomyces cerevisiae. Mol Biol Reports 32:215-226
8. Lehninger AL, Nelson DL, Cox MM (1993) Principles of Biochemistry. Worth Publisher, New York
9. Milton HS Jr (2000) A functional-phylogenetic classification system for transmembrane solute transporter. Microbiol. Mol Biol Rev 64:354-411
10. Napier J, Michaelson LV, Stobart AK (1999) Plant desaturases: harvesting the fat on land. Curr Opin Plant Biol 2:123-127
11. Raleigh EA, Leach K, Brent R (1986) In: Ausubel FM (ed) Wiley Interscience, New York
12. Sambrook J, Fritsch EF, Maniatis T (1989) Cold Spring Harbour Laboratory, Cold Spring Harbour, New York
13. Sato N, Murata N (1988) Membrane lipid, Meth Enzymol 167:251-259
14. Sperling P, Heinz E (2001) Desaturases fused to their electron donor. Eur J Lipid Sci Technol 103:158-180
15. Sperling P, Ternes P, Zank TK, Heinz E (2003) The evolution of desaturases. Prostaglandins Leukot Essent Fatty Acids 68:73-95
16. Stubbs CD, Smith AD (1984) The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. Biochimi Biophy Acta 779:89-137
17. Ta TD, Vickery LE (1992) Cloning, sequencing and overexpression of a [2Fe-2S] ferredoxin gene from Escherichia coli. J Biol Chem 267:11120-11125
18. + reductase and ferredoxin. J Biol Chem 276:5483-5490
19. Woodcock DM, Crowther PJ, Doherty J (1989) Quantitative evaluation of Escherichia coli host strain for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res 17:3496-3478