Analysis of RNA synthesis by cytometry

Methods in Cell Biology

Volumn 64, Issue 64, 2001, Pages 129-138

  Jensen, Peter Østrup ^a   Larsen, Jacob ^a   Larsen, Jørgen K ^a  

^a FINSEN LABORATORY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; 5 BROMOURIDINE; 5-BROMOURIDINE; DNA; DRUG DERIVATIVE; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENT DYE; INDOLE DERIVATIVE; RNA; TISSUE EXTRACT; URIDINE;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CYCLE; CELL LINE; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENETIC TRANSCRIPTION; HUMAN; METABOLISM; METHODOLOGY; RAT; STAINING;

ANIMALS; CELL CYCLE; CELL LINE; DNA; FLOW CYTOMETRY; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; HUMANS; INDOLES; MICROSCOPY, FLUORESCENCE; RATS; RNA; STAINING AND LABELING; TISSUE EXTRACTS; TRANSCRIPTION, GENETIC; URIDINE;

EID: 35448958557     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/s0091-679x(01)64010-x     Document Type: Article

References (26)

1. Carayon, P., and Bord, A. (1992). Identification of DNA-replicating lymphocyte subsets using a new method to label the bromodeoxyuridine incorporated into the DNA. J. Immunol. Methods 147, 225-230.
2. Carmo-Fonseca, M., Cunha, C., Custódio, N., Carvalho, C., Jordan, P., Ferreira, J., and Parreira, L. (1996). The topography of chromosomes and genes in the nucleus. Exp. Cell Res. 229, 247-252.
3. Darzynkiewicz, Z. (1994). Simultaneous analysis of cellular RNA and DNA content. Methods Cell Biol. 41A, 401-420.
4. Dolbeare F. (1995). Bromodeoxyuridine: A diagnostic tool in biology and medicine, Part 1: Historical perspectives, histochemical methods and cell kinetics. Histochem. J. 27, 339-369.
5. Dundr, M., and Raška, I. (1993). Nonisotopic ultrastructural mapping of transcription sites within the nucleolus. Exp. Cell Res. 208, 275-281.
6. Fakan, S. (1986). Structural support for RNA synthesis in the cell nucleus. Methods Achiev. Exp. Pathol. 12, 105-40.
7. Frankfurt, O. S. (1990). Flow cytometric analysis of double stranded RNA content distributions. Methods Cell Biol. 33, 299-304.
8. Haider, S. R., Juan, G., Traganos, F., and Darzynkiewicz, Z. (1997). Immunoseparation and immuno-detection of nucleic acids labeled with halogenated nucleotides. Exp. Cell Res. 234, 498-506.
9. Hassan, A. B., Errington, R. J., White, N. S., Jackson, D. A., and Cook, P. R. (1994). Replication and transcription sites are colocalized in human cells. J. Cell Sci. 107, 425-434.
10. Haukenes, G., Szilvay, A.-M., Brokstad, K. A., Kaestrøm, A., and Kalland, K.-H. (1997). Labeling of RNA transcripts of eukaryotic cells in culture with BrUTP using a liposome transfection reagent (DOTAP). BioTechniques 22, 308-312.
11. Hozák, P., Cook, P. R., Schofer, C., Mosgoller, W., and Wachtler, F. (1994). Site of transcription of ribosomal RNA and intranucleolar structure in HeLa cells. J. Cell Sci. 107, 639-648.
12. Iborra, F. J., Pombo, A., Jackson, D. A., and Cook, P. R. (1996). Active RNA polymerases are localized within transcription factories in human nuclei. J. Cell Sci. 109, 1427-1436.
13. Jackson, D. A., Hassan, A. B., Errington, R. J., and Cook, P. R. (1993). Visualization of focal sites of transcription within human nuclei. EMBO J. 12, 1059-1065.
14. Jackson, D. A., Iborra, F. J., Manders, E. M. M., and Cook, P. R. (1998). Numbers and organization of RNA polymerases, nascent transcripts, and transcription units in HeLa nuclei. Mol. Biol. Cell 9, 1523-1536.
15. Jensen, P. Ø., Larsen, J., Christiansen, J., and Larsen, J. K. (1993a). Flow cytometric measurement of RNA synthesis using bromouridine labelling and bromodeoxyuridine antibodies. Cytometry 14, 455-458.
16. Jensen, P. Ø., Larsen, J., and Larsen, J. K. (1993b). Flow cytometric measurement of RNA synthesis based on bromouridine labelling and combined with measurement of DNA content or cell surface antigen. Acta Oncol. 32, 521-524.
17. LaMorte, V. J., Dyck, J. A., Ochs, R. L., and Evans, R. M. (1998). Localization of nascent RNA and CREB binding protein with the PML-containing nuclear body. Proc. Natl. Acad. Sci. U.S.A. 95, 4991-4996.
18. Landberg, G., and Roos, G. (1991). Antibodies to proliferating cell nuclear antigen (PCNA) as S-phase specific probes in flow cytometric cell cycle analysis. Cancer Res. 51, 4570-4575.
19. Li, X., Patel, R., Melamed, M. R., and Darzynkiewicz, Z. (1994). The cell cycle effects and induction of apoptosis by 5-bromouridine in cultures of human leukemic MOLT-4 and HL-60 cell lines and mitogen stimulated normal lymphocytes. Cell Prolif. 27, 307-320.
20. Melcak, I., Risueno, M. C., and Raska, I. (1996). Ultrastructural nonisotopic mapping of nucleolar transcription sites in onion protoplasts. J. Struct. Biol. 116, 253-263.
21. Murakami, T., Li, X., Gong, J., Bhatia, U., Traganos, F., and Darzynkiewicz, Z. (1995). Induction of apoptosis by 5-azacytidine: Drug concentration-dependent differences in cell cycle specificity. Cancer Res. 55, 3093-3098.
22. Otto, F. (1990). DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA. Methods Cell Biol. 33, 105-110.
23. Pombo, A., and Cook, P. R. (1996). The localization of sites containing nascent RNA and splicing factors. Exp. Cell Res. 229, 201-203.
24. Wansink, D. G., Schul, W., van der Kraan, I., van Steensel, B., van Driel, R., and de Jong, L. (1993). Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II domains scattered throughout the nucleus. J. Cell Biol. 122, 283-293.
25. Wansink, D. G., Nelissen, R. L. H., and de Jong, L. (1994a). In vitro splicing of pre-mRNA containing bromouridine. Mol. Biol. Rep. 19, 109-113.
26. Wansink, D. G., Manders, E. E., van der Kraan, I., Aten, J. A., van Driel, R., and de Jong, L. (1994b). RNA polymerase II transcription is concentrated outside replication domains throughout S-phase. J. Cell Sci. 107, 1449-1456.