Net and gross incorporation of nitrogen by marine copepods fed on 15N-labelled diatoms: Methodology and trophic studies

Journal of Experimental Marine Biology and Ecology

Volumn 352, Issue 2, 2007, Pages 295-305

  Vincent, Dorothée ^a   Slawyk, Gerd ^b   L'Helguen, Stéphane ^c   Sarthou, Géraldine ^d   Gallinari, Morgane ^d   Seuront, Laurent ^e,f   Sautour, Benoît ^g   Ragueneau, Olivier ^d  

^a UNIVERSITÉ DU LITTORAL CÔTE D OPALE   (France)

^b STATION MARINE D ENDOUME   (France)

^c STATION BIOLOGIQUE DE ROSCOFF   (France)

^d TECHNOPÔLE BREST IROISE   (France)

^e Laboratoire d Océanologie et de Géosciences   (France)

^f FLINDERS UNIVERSITY   (Australia)

^g UNIVERSITÉ BORDEAUX 1   (France)

Author keywords

15N tracer; Copepods; N gross incorporation; N net incorporation; Nitrogen cycling

Indexed keywords

CRUSTACEAN; DISSOLVED ORGANIC NITROGEN; FEEDING; MARINE ECOSYSTEM; NET ECOSYSTEM PRODUCTION; NITROGEN; PHYTOPLANKTON; STABLE ISOTOPE; TROPHIC CASCADE;

ACARTIA DISCAUDATA; BACILLARIOPHYTA; CALANUS HELGOLANDICUS; COPEPODA; SKELETONEMA COSTATUM; THALASSIOSIRA WEISSFLOGII;

EID: 35448953617     PISSN: 00220981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jembe.2007.08.006     Document Type: Article

References (48)

1. Abou Debs C. Carbon and nitrogen budget of the calanoid copepod Temora stylifera: effect of concentration and composition of food. Mar. Ecol. Prog. Ser. 15 (1984) 213-223
2. Båmstedt U. Chemical composition and energy content. The Biological Chemistry of Marine Copepods. In: Corner E.D.S., and O' Hara S.C.M. (Eds). The Biological Chemistry of Marine Copepods (1986), Oxford University Press, New York 1-58
3. Berman T., and Bronk D.A. Dissolved organic nitrogen: a dynamic participant in aquatic ecosystems. Aquat. Microb. Ecol. 31 (2003) 279-305
4. Bonnet D., Titelman J., and Harris R. Calanus the cannibal. J. Plankton Res. 26 (2004) 937-948
5. 15N tracer method for measurement of dissolved organic nitrogen release by phytoplankton. Mar. Ecol. Prog. Ser. 77 (1991) 171-182
6. 15N differs among marine systems. Limnol. Oceanogr. 39 (1994) 189-195
7. Bronk D.A., and Ward B.B. Inorganic and organic nitrogen cycling in the Southern California Bight. Deep Sea Res. I. 52 (2005) 2285-2300
8. Butler M., and Dam H.G. Production rates and characteristics of fecal pellets of the copepod Acartia tonsa under simulated phytoplankton bloom conditions: implications for vertical fluxes. Mar. Ecol. Prog. Ser. 114 (1994) 81-91
9. Cervetto G., Gaudy R., Pagano M., Saint-Jean L., Verriopoulos G., Arfi R., and Leveau M. Diel variations in Acartia tonsa feeding, respiration and egg production in a Mediterranean coastal lagoon. J. Plankton Res. 15 (1993) 1207-1228
10. Dam H.G., and Lopes R.M. Omnivory in the calanoid copepod Temora longicornis: feeding, egg production and egg hatching rates. J. Exp. Mar. Biol. Ecol. 292 (2003) 119-137
11. Deason E.E. Grazing of Acartia hudsonica (A. clausi) on Skeletonema costatum in Narragansett Bay (USA): influence of food concentration and temperature. Mar. Biol. 60 (1980) 101-113
12. Dugdale R.C., and Goering J.J. Uptake of new and regenerated forms of nitrogen in primary productivity. Limnol. Oceanogr. 12 (1967) 196-206
13. Frangoulis C., Christou E.D., and Hecq J.-H. Comparison of marine copepod outfluxes: nature, rate, fate and role in the Carbon and Nitrogen cycles. Adv. Mar. Biol. 47 (2005) 253-309
14. Frost B.W. Effects of size and concentration of food particles on the feeding behavior of the marine planktonic copepod Calanus pacificus. Limnol. Oceanogr. 17 (1972) 805-815
15. Gaudy R. The role of zooplankton in the nitrogen cycle of Mediterranean brackish lagoon. Sci. Mar. 53 (1989) 609-616
16. Gaudy R., Pagano M., Cervetto G., Saint-Jean L., Verriopoulos G., and Beker B. Short term variations in feeding and metabolism of Acartia tonsa (pelagic copepod) in the Berre lagoon (France). Oceanol. Acta 19 (1996) 635-644
17. Gaudy R., Cervetto G., and Pagano M. Comparison of the metabolism of Acartia clausi and A. tonsa: influence of temperature and salinity. J. Exp. Mar. Biol. Ecol. 247 (2000) 51-65
18. Glibert P.M., Lipschultz F., McCarthy J.J., and Altabet M.A. Isotope dilution models of uptake and remineralization of ammonium by marine plankton. Limnol. Oceanogr. 27 (1982) 639-650
19. Guillard R.R.L., and Ryther J.H. Studies on marine planktonic diatoms. I. Cyclotella nana and Hustedt and Detonala confervaceae (Cleve). Gran. Can. J. Microbiol. 8 (1962) 229-239
20. Hasegawa T., Koike I., and Mukai H. Dissolved organic nitrogen dynamics in coastal waters and the effect of copepods. J. Exp. Mar. Biol. Ecol. 244 (2000) 219-238
21. Hasegawa T., Koike I., and Mukai H. Estimation of dissolved organic nitrogen release by micrograzers in natural planktonic assemblages. Plankton Biol. Ecol. 47 (2000) 23-30
22. Hasegawa T., Koike I., and Mukai H. Release of dissolved organic nitrogen by size-fractionated natural planktonic assemblages in coastal waters. Mar. Ecol. Prog. Ser. 198 (2000) 43-49
23. Hasegawa T., Koike I., and Mukai H. Fate of food nitrogen in marine copepods. Mar. Ecol. Prog. Ser. 210 (2001) 167-174
24. Hasegawa T., Koike I., and Mukai H. Release of dissolved organic nitrogen by a planktonic community in Akkeshi Bay. Aquat. Microb. Ecol. 24 (2001) 99-107
25. Hassett R.P., and Landry M.R. Seasonal changes in feeding rate, digestive enzyme activity, and assimilation efficiency of Calanus pacificus. Mar. Ecol. Prog. Ser. 62 (1990) 203-210
26. Hitchcock G.L. Photosynthate partitioning in cultured marine Phytoplankton. 1. Dinoflagellates. J. Exp. Mar. Biol. Ecol. 69 (1983) 21-36
27. Ikeda T., Kanno Y., Ozaki K., and Shinada A. Metabolic rates of epipelagic marine copepods as a function of body mass and temperature. Mar. Biol. 139 (2001) 587-596
28. Jones R.H., and Flynn K.J. Nutritional status and diet composition affect the value of diatoms as copepod prey. Science 307 (2005) 1457-1459
29. Koroleff F. Direct determination of ammonia. In: Grasshoff K. (Ed). Methods of seawater analysis (1969) 126-133
30. Lampert W. Release of dissolved organic carbon by grazing zooplankton. Limnol. Oceanogr. 23 (1978) 831-834
31. Marin V., Huntley M.E., and Frost B. Measuring feeding rates of pelagic herbivores: analysis of experimental design and methods. Mar. Biol. 93 (1986) 49-58
32. Mauchline J. The biology of calanoid copepods. In: Blaxter J.H.S., Southward A.J., and Tyler P.A. (Eds). Advances in Marine Biology (1998), Academic Press 660
33. Miller C.A., and Glibert P.M. Nitrogen excretion by the calanoid copepod Acartia tonsa: results of mesocosm experiments. J. Plankton Res. 20 (1998) 1767-1780
34. Mitra A., and Flynn K.J. Predator-prey interactions: is 'ecological stoichiometry' sufficient when good food goes bad?. J. Plankton Res. 27 (2005) 393-399
35. 2-labelled Trichodesmium spp. and ammonium regeneration by the harpacticoid copepod Macrosetella gracilis. Mar. Biol. 125 (1996) 89-96
36. Paffenhöfer G.-A., and Knowles S.C. Ecological implications of fecal pellet size, production and consumption by copepods. J. Mar. Res. 37 (1979) 35-49
37. Pagano M., and Saint-Jean L. In situ metabolic budget for the calanoid copepod Acartia clausi in a tropical brackish water lagoon (Ebrié Lagoon, Ivory Coast). Hydrobiol. 272 (1994) 147-161
38. Raimbault P., Slawyk G., Boudjellal B., Coatanoan C., Conan P., Coste B., Garcia N., Moutin T., and Pujo-Pay M. Carbon and nitrogen uptake in the equatorial Pacific at 150°W: evidence of an efficient regenerated production cycle. J. Geophys. Res. 104 (1999) 3341-3356
39. Rey-Rassat C., Irigoien X., Harris R., Head R., and Carlotti F. Egg production rates of Calanus helgolandicus females reared in the laboratory: variability due to present and past feeding conditions. Mar. Ecol. Prog. Ser. 238 (2002) 139-151
40. Sheppard C.W. In: Wiley J., and sons (Eds). Basic principles of the Tracer method (1962), Wiley and Sons, New York 30-47
41. 15N-tracer experiments improving the isotopic mass balance. Mar. Ecol. Prog. Ser. 124 (1995) 289-299
42. 15N losses to the dissolved organic nitrogen pool. Limnol. Oceanogr. 43 (1998) 1734-1739
43. 15N to measure dissolved organic nitrogen release by marine phytoplankton. Limnol. Oceanogr. 45 (2000) 1884-1886
44. Sournia A., and Birrien J.L. La séne océanographique côtière de Roscoff (Manche occidentale) de 1985 a 1992. Cah. Biol. Mar. 36 (1995) 1-8
45. Urban-Rich J., McCarty J.T., Fernández D., and Acuña J.L. Larvaceans and copepods excrete fluorescent dissolved organic matter (FDOM). J. Exp. Mar. Biol. Ecol. 332 (2006) 96-105
46. Vincent, D., 2002. Dynamique et nutrition du zooplancton en milieu lagunaire macrotidal (Bassin d'Arcachon): Flux de carbone et d'azote associés et conséquences sur le pool nutritif et les organismes. PhD thesis, Université de la Méditerranée, 393pp.
47. Vincent D., Luczak C., and Sautour B. Effects of a brief climatic event on zooplankton community structure and distribution in Arcachon Bay (France). J. Mar. Biol. Assoc. U.K. 82 (2002) 21-30
48. Wilkerson F.P., and Dugdale R.C. Measurements of nitrogen productivity in the Equatorial Pacific. J. Geophys.Res. 97 (1992) 669-679