Overexpression and purification of the three components of the Enterobacter aerogenes AcrA-AcrB-TolC multidrug efflux pump

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 786, Issue 1-2, 2003, Pages 197-205

  Masi, Muriel ^a   Pagès, Jean Marie ^a   Pradel, Elizabeth ^a  

^a INSERM   (France)

Author keywords

AcrA AcrB TolC multidrug efflux pump; Enterobacter aerogenes; Expression; Purification

Indexed keywords

GEL PERMEATION CHROMATOGRAPHY; MICROORGANISMS; PURIFICATION;

INNER MEMBRANE PROTEINS;

PROTEINS;

EDETIC ACID; HISTIDINE DERIVATIVE; IMIDAZOLE; MEMBRANE PROTEIN; TYLOXAPOL;

AMINO TERMINAL SEQUENCE; ARTIFICIAL MEMBRANE; CARBOXY TERMINAL SEQUENCE; CELL MEMBRANE TRANSPORT; CONFERENCE PAPER; CONTROLLED STUDY; CYTOPLASM; ENTEROBACTER AEROGENES; ESCHERICHIA COLI; EXPRESSION VECTOR; GEL PERMEATION CHROMATOGRAPHY; GENE INSERTION; GENE OVEREXPRESSION; GENETIC CODE; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; OPEN READING FRAME; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PURIFICATION; SOLUBILITY; ULTRACENTRIFUGATION; WESTERN BLOTTING;

ENTEROBACTER; ENTEROBACTER AEROGENES; ESCHERICHIA COLI; VECTORS;

EID: 0037465672     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1570-0232(02)00746-8     Document Type: Conference Paper

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