Mutagenesis of the cysteine residues in the transcription factor NtcA from Anabaena PCC 7120 and its effects on DNA binding in vitro

Biochimica et Biophysica Acta - Gene Structure and Expression

Volumn 1679, Issue 2, 2004, Pages 156-163

  Wisén, Susanne ^a   Bergman, Birgitta ^b   Mannervik, Bengt ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

Cyanobacteria; Cysteine residue; DNA binding; NtcA; Transcription factor

Indexed keywords

CYCLIC AMP BINDING PROTEIN; CYSTEINE; DISULFIDE; DNA; MUTANT PROTEIN; PALINDROMIC DNA; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR NTCA; UNCLASSIFIED DRUG;

ANABAENA; ARTICLE; DISULFIDE BOND; DNA BINDING; DNA PROBE; GEL MOBILITY SHIFT ASSAY; IN VITRO STUDY; MOLECULAR MODEL; MUTAGENESIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; WILD TYPE;

ANABAENA; BACTERIAL PROTEINS; CYSTEINE; DITHIOERYTHRITOL; DNA-BINDING PROTEINS; ELECTROPHORETIC MOBILITY SHIFT ASSAY; FLUORESCENT DYES; MODELS, MOLECULAR; MUTAGENESIS, INSERTIONAL; TRANSCRIPTION FACTORS;

ANABAENA; CYANOBACTERIA; NOSTOC SP. PCC 7120;

EID: 3543075724     PISSN: 01674781     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbaexp.2004.06.003     Document Type: Article

References (32)

1. Flores E., Herrero A. Bryant D.A. The Molecular Biology of Cyanobacteria. 1994;487-517 Kluwer Academic Publishers, London
2. Herrero A., Muro-Pastor A.M., Flores E. Nitrogen control in cyanobacteria. J. Bacteriol. 183:2001;411-425
3. Wolk C.P., Ernst A., Elhai J. Bryant D.A. The Molecular Biology of Cyanobacteria. 1994;769-823 Kluwer Academic Publishers, London
4. Golden J.W., Robinson S.J., Haselkorn R. Rearrangement of nitrogen fixation genes during heterocyst differentiation in the cyanobacterium Anabaena. Nature. 314:1985;419-423
5. Haselkorn R. The heterocyst. Annu. Rev. Plant Physiol. 29:1978;319-344
6. Frias J.E., Merida A., Herrero A., Martin-Nieto J., Flores E. General distribution of the nitrogen control gene ntcA in cyanobacteria. J. Bacteriol. 175:1993;5710-5713
7. Lindell D., Padan E., Post A.F. Regulation of ntcA expression and nitrite uptake in the marine Synechococcus sp. strain WH 7803. J. Bacteriol. 180:1998;1878-1886
8. Frias J.E., Flores E., Herrero A. Requirement of the regulatory protein NtcA for the expression of nitrogen assimilation and heterocyst development genes in the cyanobacterium Anabaena sp. PCC 7120. Mol. Microbiol. 14:1994;823-832
9. Wei T.F., Ramasubramanian T.S., Pu F., Golden J.W. Anabaena sp. strain PCC 7120 bifA gene encoding a sequence-specific DNA-binding protein cloned by in vivo transcriptional interference selection. J. Bacteriol. 175:1993;4025-4035
10. Harrison S.C., Aggarwal A.K. DNA recognition by proteins with the helix-turn-helix motif. Annu. Rev. Biochem. 59:1990;933-969
11. Jiang F., Wisén S., Widersten M., Bergman B., Mannervik B. Examination of the transcription factor NtcA-binding motif by in vitro selection of DNA sequences from a random library. J. Mol. Biol. 301:2000;783-793
12. Luque I., Flores E., Herrero A. Molecular mechanism for the operation of nitrogen control in cyanobacteria. EMBO J. 13:1994;2862-2869
13. Ramasubramanian T.S., Wei T.F., Golden J.W. Two Anabaena sp. strain PCC 7120 DNA-binding factors interact with vegetative cell- and heterocyst-specific genes. J. Bacteriol. 176:1994;1214-1223
14. Ramasubramanian T.S., Wei T.F., Oldham A.K., Golden J.W. Transcription of the Anabaena sp. strain PCC 7120 ntcA gene: multiple transcripts and NtcA binding. J. Bacteriol. 178:1996;922-926
15. Jiang F., Hellman U., Sroga G.E., Bergman B., Mannervik B. Cloning, sequencing, and regulation of the glutathione reductase gene from the cyanobacterium Anabaena PCC 7120. J. Biol. Chem. 270:1995;22882-22889
16. Paz-Yepes J., Flores E., Herrero A. Transcriptional effects of the signal transduction protein P(II) (glnB gene product) on NtcA-dependent genes in Synechococcus sp. PCC 7942. FEBS Lett. 543:2003;42-46
17. Fadi Aldehni M., Sauer J., Spielhaupter C., Schmid R., Forchhammer K. Signal transduction protein P(II) is required for NtcA-regulated gene expression during nitrogen deprivation in the cyanobacterium Synechococcus elongatus strain PCC 7942. J. Bacteriol. 185:2003;2582-2591
18. Muro-Pastor M.I., Reyes J.C., Florencio F.J. Cyanobacteria perceive nitrogen status by sensing intracellular 2-oxoglutarate levels. J. Biol. Chem. 276:2001;38320-38328
19. Vazquez-Bermudez M.F., Herrero A., Flores E. 2-Oxoglutarate increases the binding affinity of the NtcA (nitrogen control) transcription factor for the Synechococcus glnA promoter. FEBS Lett. 512:2002;71-74
20. Vazquez-Bermudez M.F., Herrero A., Flores E. Carbon supply and 2-oxoglutarate effects on expression of nitrate reductase and nitrogen-regulated genes in Synechococcus sp. strain PCC 7942. FEMS Microbiol. Lett. 221:2003;155-159
21. Tanigawa R., Shirokane M., Maeda Si S., Omata T., Tanaka K., Takahashi H. Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro. Proc. Natl. Acad. Sci. U. S. A. 99:2002;4251-4255
22. Stanier R.Y., Cohen-Bazire G. Phototrophic prokaryotes: the cyanobacteria. Annu. Rev. Microbiol. 31:1977;225-274
23. Jiang F., Mannervik B., Bergman B. Evidence for redox regulation of the transcription factor NtcA, acting both as an activator and a repressor, in the cyanobacterium Anabaena PCC 7120. Biochem. J. 327:1997;513-517
24. Alfonso M., Perewoska I., Kirilovsky D. Redox control of ntcA gene expression in Synechocystis sp. PCC 6803. Nitrogen availability and electron transport regulate the levels of the NtcA protein. Plant Physiol. 125:2001;969-981
25. Wisén S., Jiang F., Bergman B., Mannervik B. Expression and purification of the transcription factor NtcA from the cyanobacterium Anabaena PCC 7120. Protein Expr. Purif. 17:1999;351-357
26. Riddles P.W., Schnitzerling H.J., Davey P.A. Application of trans and cis isomers of p-nitrophenyl-(1R, S)-3-(2,2-dichlorovinyl)-2,2- dimethylcyclopropanecarboxylate to the assay of pyrethroid-hydrolyzing esterases. Anal. Biochem. 132:1983;105-109
27. Passner J.M., Schultz S.C., Steitz T.A. Modeling the cAMP-induced allosteric transition using the crystal structure of CAP-cAMP at 2.1 Å resolution. J. Mol. Biol. 304:2000;847-859
28. Adair G.S. The oxygen dissociation curve of hemoglobin. J. Biol. Chem. 63:1925;529-545
29. Augustinsson K.B., Bartfai T., Mannervik B. A steady-state kinetic model of butyrylcholinesterase from horse plasma. Biochem. J. 141:1974;825-834
30. Aiba H., Fujimoto S., Ozaki N. Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein. Nucleic Acids Res. 10:1982;1345-1361
31. McKay D.B., Steitz T.A. Structure of catabolite gene activator protein at 2.9 Å resolution suggests binding to left-handed B-DNA. Nature. 290:1981;744-749
32. de Crombrugghe B., Busby S., Buc H. Cyclic AMP receptor protein: role in transcription activation. Science. 224:1984;831-838