Influence of coupling method on the luminescence properties, coupling efficiency, and binding affinity of antibodies labeled with europium(III) chelates

Bioconjugate Chemistry

Volumn 8, Issue 1, 1997, Pages 71-75

  Karşilayan, Huriye ^a   Hemmilä, Ilkka ^b   Takalo, Harri ^b   Toivonen, Airi ^b   Pettersson, Kim ^c   Lövgren, Timo ^c   Mukkala, Veli Matti ^b,c  

^a YILDIZ TECHNICAL UNIVERSITY   (Turkey)

^b Wallac Oy   (Finland)

^c UNIVERSITY OF TURKU   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ENERGY; CHELATION; EUROPIUM COMPOUNDS; LUMINESCENCE;

BINDING AFFINITIES; COUPLING EFFICIENCY; COUPLING METHODS; EUROPIUM CHELATE; LUMINESCENCE PROPERTIES; PARA POSITION; PHENYL RINGS; PHENYLETHYNYL; SYNTHESISED;

ANTIBODIES;

EID: 0031048186     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc960075l     Document Type: Article

References (21)

1. Hemmilä, I. (1990) Application of Fluorescence in Immunoassays, Wiley, New York.
2. Hemmilä, I., Ståhlberg, T., and Mottram, P. (Eds.) (1994) Bioanalytical Applications of Labeling Technologies, Wallac, Turku.
3. Soini, E., and Hemmilä, I. (1979) Fluoroimmunoassay: present status and key problems. Clin. Chem. 25, 353-361.
4. Hemmilä, I. (1988) Lanthanides as probes for time-resolved fluorometric immunoassays. Scand. J. Clin. Lab. Invest. 4, 389-400.
5. Mukkala, V.-M., Mikola, H., and Hemmilä, I. (1989) The synthesis and use of activated N-benzyl derivatives of diethylenetriaminetetraacetic acids: alternative reagents for labeling of antibodies with metal ions. Anal. Biochem. 176, 319-325.
6. Hemmilä, I., Dakubu, S., Mukkala, V.-M., Siitari, H., and Lövgren, T. (1984) Europium as a label in time-resolved immunofluorometric assays. Anal. Biochem. 137, 335-343.
7. Evangelista, R. A., Pollak, A., Allore, B., Templeton, E. F., Morton, R. C., and Diamandis, E. P. (1988) A new europium chelate for protein labeling and time-resolved fluorescence applications. Clin. Biochem. 21, 173-178.
8. Gudgin Dickson, E. F., Pollak, A., and Diamandis, E. P. (1995) Time-resolved detection of lanthanide luminescence for ultrasensitive bioanalytical assays. J. Photochem. Photobiol. B:Biol. 27, 3-19.
9. Mukkala, V.-M., Sund, C., Kwiatkowski, M., Pasanen, P., Högberg, M., Kankare, J., and Takalo, H. (1992) New heteroaromatic complexing agents and luminescence of their europium(III) and terbium(III) chelates. Helv. Chim. Acta 75, 1621-1632.
10. Takalo, H., Hänninen, E., and Kankare, J. (1993) Luminescence of Eu(III) chelates of substituted 4-phenylethynylpyridines as ligands. Helv. Chim. Acta 76, 877-883.
11. Mikola, H., Takalo, H., and Hemmilä, I. (1995) Syntheses and properties of luminescent chelate labels and labeled haptenic antigens for homogenic immunoassays. Bioconjugate Chem. 6, 235-241.
12. Mukkala, V.-M., Helenius, M., Hemmilä, I., Kankare, J., and Takalo, H. (1993) Development of luminescent europium(III) and terbium (III) chelates of 2,2′:6′,2″-terpyridine derivatives for protein labeling. Helv. Chim. Acta 76, 1-18.
13. Takalo, H., Mukkala, V.-M., Mikola, H., Liitti, P., and Hemmilä, I. (1994) Synthesis of europium(III) chelates suitable for labeling of bioactive molecules. Bioconjugate Chem. 5, 278-282.
14. Hemmilä, I., Mukkala, V.-M., and Takalo, H. (1996) Development of lanthanide chelate labels for diagnostic assays. J. Alloys Compd. (in press).
15. Latva, M., Takalo, H., Mukkala, V.-M., Matachescu, C., Rodriguez-Ubis, J. C., and Kankare, J. (1996) Correlation between luminescence efficiencies of europmm(III) and terbium(III) chelates and triplet state energy levels. J. Lumin. (submitted for publication).
16. Takalo, H., Mukkala, V.-M., Meriö, L., Rodriguez-Ubis, J. C., Sedano, R., Juanes, O., and Brunet, E. (1996) Development of luminescent terbium(III) chelates for protein labeling-effect of triplet state energy level. Helv. Chim. Acta (submitted for publication).
17. Mitrunen, K., Pettersson K., Piironen, T., Bjork, T., Lilja, H., and Lövgren, T. (1995) A novel dual-label one-step immunoassay for simultaneous measurement of free and total PSA concentrations and ratios in serum. Clin. Chem. 41, 1115-1120.
18. Pettersson, K. S. I., and Söderholm, J. M. (1991) Individual differences in lutropin immunoreactivity revealed by monoclonal antibodies. Clin. Chem. 37, 333-340.
19. Scatchard, G. (1949) The attractions of proteins for small molecules and ions. Ann. N. Y. Acad. Sci. 51, 660-672.
20. Pettersson, K. (1990) Immunofluoromeric assays of LH with special reference to sensitivity and specificity. Ph.D. Thesis, Åbo Akademi, Turku, Finland.
21. De W. Horrocks, W., Jr., and Sudnick, D. S. (1981) Lanthanide ion luminescence probes of the structure of biological macromolecules. Acc. Chem. Res. 14, 384-392.