Cloning, site-directed mutagenesis and expression of cathepsin L-like cysteine protease from Uronema marinum (Ciliophora: Scuticociliatida)

Molecular and Biochemical Parasitology

Volumn 156, Issue 2, 2007, Pages 191-198

  Ahn, Sang Jung ^a   Seo, Jung Soo ^b   Kim, Moo Sang ^a   Jeon, Soo Jin ^a   Kim, Na Young ^a   Jang, Jae Ho ^a   Kim, Ki Hong ^a   Hong, Yong Ki ^a   Chung, Joon Ki ^a   Lee, Hyung Ho ^a  

^a Pukyong National University   (South Korea)

^b National Fisheries Research and Development Institute (NFRDI)   (South Korea)

Author keywords

Cathepsin L; Ciliate; Cysteine protease; Scuticociliate; Site directed mutagenesis; Uronema marinum

Indexed keywords

CATHEPSIN L; COMPLEMENTARY DNA; CYSTEINE PROTEINASE; LEUPEPTIN; N [N (3 CARBOXYOXIRANE 2 CARBONYL)LEUCYL]AGMATINE; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; DNA LIBRARY; DOT HYBRIDIZATION; ENZYME ACTIVE SITE; ENZYME INHIBITION; ENZYME PURIFICATION; EXPRESSION VECTOR; GENE; GENE ISOLATION; GENETIC CODE; HETEROLOGOUS EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTOZOON; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; URONEMA MARINUM;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; CYSTEINE ENDOPEPTIDASES; CYSTEINE PROTEINASE INHIBITORS; DNA, PROTOZOAN; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE LIBRARY; HYDROGEN-ION CONCENTRATION; LEUCINE; LEUPEPTINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OLIGOHYMENOPHOREA; PROTEIN FOLDING; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

CILIOPHORA; EUKARYOTA; SCUTICOCILIATIA; URONEMA MARINUM;

EID: 35348932095     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.molbiopara.2007.07.021     Document Type: Article

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