Molecular cloning and expression of a cDNA encoding a Coccidioides posadasii Cu,Zn superoxide dismutase identified by proteomic analysis of the coccidioidal T27K Vaccine

Annals of the New York Academy of Sciences

Volumn 1111, Issue , 2007, Pages 181-197

  Lunetta, Jennine M ^a   Simmons, Keira A ^a   Johnson, Suzanne M ^a   Pappagianis, Demosthenes ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Coccidioides; Fungus; Mass spectrometry; Metalloenzyme; Rapid amplification of cDNA ends; Reverse transcription polymerase chain reaction; SOD1; Superoxide dismutase; Two dimensional gel electrophoresis; Vaccine

Indexed keywords

COMPLEMENTARY DNA; COPPER ZINC SUPEROXIDE DISMUTASE; FUNGAL ENZYME; FUNGAL PROTEIN; FUNGUS VACCINE; MYOCILIN; PEPTIDE; RECOMBINANT PROTEIN;

COCCIDIOIDES; COCCIDIOIDES POSADASII; CONFERENCE PAPER; DNA SEQUENCE; GENE; GENE EXPRESSION; MASS SPECTROMETRY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEOMICS;

COCCIDIOIDES; COCCIDIOIDES POSADASII; FUNGI; MUS;

EID: 35348868584     PISSN: 00778923     EISSN: 17496632     Source Type: Book Series    
DOI: 10.1196/annals.1406.025     Document Type: Conference Paper

References (22)

1. PAPPAGIANIS, D. 2001. Seeking a vaccine against Coccidioides immitis and serologic studies: expectations and realities. Fungal Genet. Biol. 32: 1-9.
2. KIRKLAND, T. & G.T. COLE. 2002. Coccidioidomycosis: pathogenesis, immune response and vaccine development. In Fungal Pathogenesis: Principles and Clinical Applications. R. A. Calderone & R. L. Cihlar, Eds.: 365-400. Marcel Dekker. New York.
3. COLE, G.T., J.-M. XUE, C.N. OKEKE, et al. 2004. A vaccine against coccidioidomycosis is justified and attainable. Med. Mycol. 42: 189-216.
4. MAGEE, D.M. & R.A. COX. 2004. Vaccine development for coccidioidomycosis. In The Mycota XII Human Fungal Pathogens. J. E. Domer & G. S. Kobayashi, Eds.: 243-257. Springer-Verlag. Heidelberg, Germany.
5. ZIMMERMANN, C.R., S.M. JOHNSON, G.W. MARTENS, et al. 1998. Protection against lethal murine coccidioidomycosis by a soluble vaccine from spherules. Infect. Immun. 66: 2342-2345.
6. FISHER, M.C., G.L. KOENIG, T.J. WHITE & J.W. TAYLOR. 2002. Molecular and phenotypic description of Coccidioides posadasii sp. nov., previously recognized as the non-California population of Coccidioides immitis. Mycologia 94: 73-84.
7. LAEMMLI, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
8. SHEVCHENKO, A., M. WILM, O. VORM & M. MANN. 1996. Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels. Anal. Chem. 68: 850-858.
9. + RNA from Coccidioides immitis spherule/endospore and hyphal phases. In Molecular Biology of Pathogenic Fungi. B. Marcesa & G. S. Kobayashi, Eds.: 257-263. Telos Press. New York.
10. TOWBIN, H., T. STAEHELIN & J. GORDEN. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedures and some applications. Proc. Natl. Acad. Sci. USA 76: 4350-4354.
11. BEAUCHAMP, C. & I. FRIDOVICH. 1971. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal. Biochem. 44: 276-287.
12. BORDO, D., K. DJINOVIC & M. BOLOGNESI. 1994. Conserved patterns in the Cu,Zn superoxide dismutase family. J. Mol. Biol. 238: 366-386.
13. HAMILTON, A. J. & M.D. HOLDOM. 1999. Antioxidant systems in the pathogenic fungi of man and their role in virulence. Med. Mycol. 37: 375-389.
14. COX, G.M., T.S. HARRISON., H.C. MCD ADE, et al. 2003. Superoxide dismutase influences the virulence of Cryptococcus neoformans by affecting growth within macrophages. Infect. Immun. 71: 173-180.
15. HWANG, C.-S., G.-E. RHIE, J.-H. OH, et al. 2002. Copper- and zinc-containing superoxide dismutase (Cu/ZnSOD) is required for the protection of Candida albicans against oxidative stresses and the expression of its full virulence. Microbiology 148: 3705-3713.
16. MARTCHENKO, M., A.-M. ALARCO, D. HARCUS & M. WHITEWAY. 2004. Superoxide dismutases in Candida albicans: transcriptional regulation and functional characterization of the hyphal-induced SOD5 gene. Mol. Biol. Cell. 15: 456-467.
17. PAN, S. & G.T. COLE. 1995. Molecular and biochemical characterization of a Coccidioides immitis-specific antigen. Infect. Immun. 63: 3994-4002.
18. ZIMMERMANN, C.R., S.M. JOHNSON, G.W. MARTENS, et al. 1996. Cloning and expression of the complement fixation antigen-chitinase of Coccidioides immitis. Infect. Immun. 64: 4967-4975.
19. THOMAS, P.W., E.E. WYCKOFF, E.J. PISHKO, et al. 1997. The hsp60 gene of the human pathogenic fungus Coccidioides immitis encodes a T-cell reactive protein. Gene 199: 83-91.
20. DELGADO, N. & G.T. COLE. 2003. A recombinant β-1,3-glucanosyltransferase homolog of Coccidioides posadasii protects mice against coccidiodomycosis. Infect. Immun. 71: 3010-3019.
21. IVEY, F.D., D.M. MAGEE, M.D. WOITASKE, et al. 2003. Identification of a protective antigen of Coccidioides immitis by expression library immunization.Vaccine 21: 4359-4367.
22. ORSBORN, K.I., L.F. SHUBITZ, T. PENG, et al. Protein expression profiling of Coccidioides posadasii by two-dimensional differential in-gel electrophoresis and evaluation of a newly recognized peroxisomal matrix protein as a recombinant vaccine candidate. Infect. Immun. 74: 1865-1872.