Production of red-flowered plants by genetic engineering of multiple flavonoid biosynthetic genes

Plant Cell Reports

Volumn 26, Issue 11, 2007, Pages 1951-1959

  Nakatsuka, Takashi ^a   Abe, Yoshiko ^a   Kakizaki, Yuko ^a   Yamamura, Saburo ^a   Nishihara, Masahiro ^a  

^a IWATE BIOTECHNOLOGY RESEARCH CENTER   (Japan)

Author keywords

Flower color; Genetic engineering; Pelargonidin; RNAi; Tobacco

Indexed keywords

DIHYDROFLAVONOL 4-REDUCTASE (DFR); FLAVONOIDS; FLORICULTURAL PLANTS; PETUNIA HYBRIDA;

BIOSYNTHESIS; ENZYME ACTIVITY; GENE EXPRESSION; RNA; TOBACCO;

GENETIC ENGINEERING;

ALCOHOL DEHYDROGENASE; ANTHOCYANIN; AROMADEDRIN; CYTOCHROME P450; DIHYDROFLAVANOL 4 REDUCTASE; DIHYDROFLAVANOL 4-REDUCTASE; FLAVONOID; FLAVONOID 3' HYDROXYLASE; FLAVONOID 3'-HYDROXYLASE; FLAVONOL SYNTHASE; MIXED FUNCTION OXIDASE; OXIDOREDUCTASE; PELARGONIDIN; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ARTICLE; FLOWER; GENETICS; MUTATION; PETUNIA; PHYSIOLOGY; TOBACCO; TRANSGENIC PLANT;

ALCOHOL OXIDOREDUCTASES; ANTHOCYANINS; CYTOCHROME P-450 ENZYME SYSTEM; FLAVONOIDS; FLOWERS; MIXED FUNCTION OXYGENASES; MUTATION; OXIDOREDUCTASES; PETUNIA; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; TOBACCO;

GERBERA; NICOTIANA OBTUSIFOLIA; NICOTIANA TABACUM; PETUNIA; PETUNIA X HYBRIDA;

EID: 35248888020     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-007-0401-0     Document Type: Article

References (37)

1. Abbott A (2006) Youthful duo snags a swift Nobel for RNA control of genes. Nature 443:488
2. Aharoni A, De Vos CH, Wein M, Sun Z, Greco R, Kroon A, Mol JN, O'Connell AP (2001) The strawberry FaMYB1 transcription factor suppresses anthocyanin and flavonol accumulation in transgenic tobacco. Plant J 28:319-332
3. Beld M, Martin C, Huits H, Stuitje AR, Gerats AG (1989) Flavonoid synthesis in Petunia hybrida: partial characterization of dihydroflavonol-4- reductase genes. Plant Mol Biol 13:491-502
4. Brugliera F, Barri-Rewell G, Holton TA, Mason JG (1999) Isolation and characterization of a flavonoid 3'-hydroxylase cDNA clone corresponding to the Ht1 locus of Petunia hybrida. Plant J 19:441-451
5. Chuang CF, Meyerowitz EM (2000) Specific and heritable genetic interference by double-stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci USA 97:4985-4990
6. Couzin J (2006) Nobel Prize in Physiology or Medicine. Method to silence genes earns loud praise. Science 314:34
7. Davies KM, Schwinn KE, Deroles SC, Manson DG, Lewis DH, Bloor SJ, Bradley JM (2003) Enhancing anthocyanin production by altering competition for substrate between flavonol synthase and dihydroflavonol 4-reductase. Euphytica 131:259-268
8. Elomaa P, Helariutta Y, Griesbach RJ, Kotilainen M, Seppanen P, Teeri TH (1995) Transgene inactivation in Petunia hybrida is influenced by the properties of the foreign gene. Mol Gen Genet 248:649-656
9. Forkmann G, Martens S (2001) Metabolic engineering and applications of flavonoids. Curr Opin Biotech 12:155-160
10. Forkmann G, Ruhnau B (1987) Distinct substrate specificity of dihydroflavonol 4-reductase from flowers of Petunia hybrida. Z Naturforsch 42:1146-1148
11. Fukusaki E, Kawasaki K, Kajiyama S, An CI, Suzuki K, Tanaka Y, Kobayashi A (2004) Flower color modulations of Torenia hybrida by downregulation of chalcone synthase genes with RNA interference. J Biotechnol 111:229-240
12. Grotewold E (2006) The genetics and biochemistry of floral pigments. Annu Rev Plant Biol 57:761-780
13. Hannon GJ (2003) RNAi: a guide to gene silencing. Cold Spring Harbor Laboratory Press, New York
14. Helariutta Y, Elomaa P, Kotilainen M, Seppanen P, Teeri TH (1993) Cloning of cDNA coding for dihydroflavonol-4-reductase (DFR) and characterization of dfr expression in the corollas of Gerbera hybrida var. Regina (Compositae). Plant Mol Biol 22:183-193
15. Horsch RB, Fry JE, Hoffmann NL, Eicholtz D, Rogers SG, Fraley RT (1985) A simple method for transferring genes into plants. Science 227:1229-1231
16. Johnson ET, Yi H, Shin B, Oh BJ, Cheong H, Choi G (1999) Cymbidium hybrida dihydroflavonol 4-reductase does not efficiently reduce dihydrokaempferol to produce orange pelargonidin-type anthocyanins. Plant J 19:81-85
17. Johnson ET, Ryu S, Yi H, Shin B, Cheong H, Choi G (2001) Alteration of a single amino acid changes the substrate specificity of dihydroflavonol 4-reductase. Plant J 25:325-333
18. Meyer P, Heidmann I, Forkmann G, Saedler H (1987) A new petunia flower colour generated by transformation of a mutant with a maize gene. Nature 330:677-678
19. Miki D, Itoh R, Shimamoto K (2005) RNA silencing of single and multiple members in a gene family of rice. Plant Physiol 138:1903-1913
20. Mol J, Cornish E, Mason J, Koes R (1999) Novel coloured flowers. Curr Opin Biotechnol 10:198-201
21. Nakamura N, Fukuchi-Mizutani M, Miyazaki K, Suzuki K, Tanaka Y (2006) RNAi suppression of the anthocyanidin synthase gene in Torenia hybrida yields white flowers with higher frequency and better stability than antisense and sense suppression. Plant Biotechnol 23:13-18
22. Nakatsuka T, Nishihara M, Mishiba K, Yamamura S (2005) Temporal expression of flavonoid biosynthesis-related genes regulates flower pigmentation in gentian plants. Plant Sci 168:1309-1318
23. Nakatsuka T, Nishihara M, Mishiba K, Yamamura S (2006) Heterologous expression of two gentian cytochrome P450 genes can modulate the intensity of flower pigmentation in transgenic tobacco plants. Mole Breed 17:91-99
24. Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genes in trans. Plant Cell 2:279-289
25. Nielsen K, Deroles SC, Markham KR, Bradley MJ, Podivinsky E, Manson D (2002) Antisense flavonol synthase alters copigmentation and flower color in lisianthus. Mole Breed 9:217-229
26. Nishihara M, Nakatsuka T, Yamamura S (2005) Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene. FEBS Lett 579:6074-6078
27. Ohta S, Mita S, Hattori T, Nakamura K (1990) Construction and expression in tobacco of a ß-glucuronidase (GUS) reporter gene containing an intron within the coding sequence. Plant Cell Physiol 31:805-813
28. Schwarz-Sommer Z, Shepherd N, Tacke E, Gierl A, Rohde W, Leclercq L, Mattes M, Berndtgen R, Peterson PA, Saedler H (1987) Influence of transposable elements on the structure and function of the A1 gene of Zea mays. EMBO J 6:287-294
29. Seitz C, Vitten M, Steinbach P, Hartl S, Hirsche J, Rathje W, Treutter D, Forkmann G (2007) Redirection of anthocyanin synthesis in Osteospermum hybrida by a two-enzyme manipulation strategy. Phytochemistry 68:824-833
30. Suzuki K, Xue HM, Tanaka Y, Fukui Y, Fukuchi-Mizutani M, Murakami Y, Katsumoto Y, Tsuda S, Kusumi T (2000) Flower color modifications of Torenia hybrida by cosuppression of anthocyanin biosynthesis genes. Mole Breed 6:239-246
31. Tanaka Y, Fukui Y, Fukuchi-Mizutani M, Holton TA, Higgins E, Kusumi T (1995) Molecular cloning and characterization of Rosa hybrida dihydroflavonol 4-reductase gene. Plant Cell Physiol 36:1023-1031
32. Tanaka Y, Yonekura K, Fukuchi-Mizutani M, Fukui Y, Fujiwara H, Ashikari T, Kusumi T (1996) Molecular and biochemical characterization of three anthocyanin synthetic enzymes from Gentiana triflora. Plant Cell Physiol 37:711-716
33. Tanaka Y, Tsuda S, Kusumi T (1998) Metabolic engineering to modify flower color. Plant Cell Physiol 39:1119-1126
34. Tanaka Y, Katsumoto Y, Brugliera F, Mason J (2005) Genetic engineering in floriculture. Plant Cell Tiss Org Cult 80:1-24
35. Tsuda S, Fukui Y, Nakamura N, Katsumoto Y, Yonekura-Sakakibara K, Fukuchi-Mizutani M, Ohira K, Ueyama Y, Ohkawa H, Holton TA, Kusumi T, Tanaka Y (2004) Flower color modification of Petunia hybrida commercial varieties by metabolic engineering. Plant Biotechnol 21:377-386
36. van der Krol AR, Lenting PJ, Veenstra JG, van der Meer IM, Koes RE, Gerats AGM, Mol JNM, Stuitje AR (1988) An antisense chalcone synthase gene in transgenic plants inhibits flower pigmentation. Nature 333:866-869
37. Waterhouse PM, Graham MW, Wang MB (1998) Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc Natl Acad Sci USA 95:13959-13964