Effect of pre-filtration on selective isolation of tat protein by affinity membrane separation: Analysis of flux, separation efficiency, and processing time

Separation Science and Technology

Volumn 42, Issue 11, 2007, Pages 2451-2471

  Datta, Saurav ^a   Bhattacharyya, D ^a   Ray, P D ^b   Nath, A ^c   Toborek, M ^d  

^a UNIVERSITY OF KENTUCKY   (United States)

^b UNIVERSITY OF KENTUCKY   (United States)

^c JOHNS HOPKINS UNIVERSITY   (United States)

^d UNIVERSITY OF KENTUCKY   (United States)

Author keywords

Affinity separation; Avidin; Biotin; Flux; Processing time; Separation efficiency; Tat

Indexed keywords

BACTERIA; IMPURITIES; MATHEMATICAL MODELS; MICROFILTRATION; MOLECULAR WEIGHT; PURIFICATION;

AFFINITY SEPARATION; AVIDIN; BIOTIN; PROCESSING TIME; SEPARATION EFFICIENCY; TAT;

PROTEINS;

BACTERIA; IMPURITIES; MATHEMATICAL MODELS; MICROFILTRATION; MOLECULAR WEIGHT; PROTEINS; PURIFICATION;

MEMBRANE; MOLECULAR WEIGHT; PROTEIN; PURIFICATION; SEPARATION;

EID: 35248862587     PISSN: 01496395     EISSN: 15205754     Source Type: Journal    
DOI: 10.1080/01496390701477121     Document Type: Conference Paper

References (32)

1. Brandt, S., Goffe, R.A., Kessler, S.B., O'Connor, J.L., and Zale, S.E. (1988) Membrane-based affinity technology for commercial purifications. Bio/Technology, 6: 779-782.
2. Briefs, K.G. and Kula, M.R. (1992) Fast protein chromatography on analytical and preparative scale using modified microporous membranes. Chem. Engg. Sci., 47: 141-149.
3. Thommes, J. and Kula, M.-R. (1995) Membrane chromatography- an integrative concept in the downstream processing of proteins. Biotechnol. Prog., 11: 357-367.
4. Suen, S.Y. and Etzel, M.R. (1992) A mathematical analysis of affinity membrane bioseparations. Chem. Engg. Sci., 47: 1355-1364.
5. Charcosset, C. (1998) Purification of proteins by membrane chromatography. J. Chem. Technol. Biotechnol., 71: 95-110.
6. Klein, E. (2000) Affinity membranes: a 1.0-year review. J. Membr. Sci., 179:1-27.
7. Klein, E. (1991) Affinity Membranes; Wiley: New York.
8. Zou, H., Luo, Q., and Zhou, D. (2001) Affinity membrane chromatography for the analysis and purification of proteins. J. Biochem. Biophys. Methods, 49: 199-240.
9. Hollman, A.M., Christian, D.A., Ray, P.D., Galey, D., Turchan, J., Nath, A., and Bhattacharyya, D. (2005) Selective isolation and purification of Tat protein via affinity membrane separation. Biotechnol. Prog., 21: 451-459.
10. Datta, S., Ray, P.D., Nath, A., and Bhattacharyya, D. (2006) Recognition based separation of HIV-Tat protein using avidin-biotin interaction in modified microfiltration membranes. J. Membr. Sci., 280: 298-310.
11. Urmenyi, A.M., Poot, A.A., Wessling, M., and Mulder, M.H.V. (2005) Affinity membranes for hormone removal from aqueous solutions. J. Membr. Sci., 259: 91-102.
12. Ruckenstein, E. and Guo, W. (2004) Cellulose and glass fiber affinity membranes for the chromatographic separation of biomolecules. Biotechnol. Prog., 20: 13-25.
13. Castilho, L.R., Deckwer, W.D., and Anspach, F.B. (2000) Influence of matrix activation and polymer coating on the purification of human IgG with protein A affinity membranes. J. Membr. Sci., 172: 269-277.
14. Bowen, W.R. and Gan, Q. (1992) Properties of microfiltration membranes: The effects of adsorption and shear on the recovery of an enzyme. Biotech. Bioeng., 40:491-497.
15. Bowen, W.R. and Gan, Q. (1991) Properties of microfiltration membranes: Flux loss during constant pressure permeation of bovine serum albumin. Biotech. Bioeng., 38: 688-696.
16. Suki, A., Fane, A.G., and Fell, C.J.D. (1984) Flux decline in protein ultrafiltration. J. Membr. Sci., 21: 269-283.
17. Bowen, W.R., Calvo, J.I., and Hernandez, A. (1995) Steps of membrane blocking in flux decline during protein microfiltration. J. Membr. Sci., 101: 153-165.
18. Velasco, C., Ouammou, M., Calvo, J.I., and Hernandez, A. (2003) Protein fouling in microfiltration: Deposition mechanism as a function of pressure for different pH. J. Colloid Interface Sci., 266: 148-152.
19. Marshal, A.D., Munro, P.A., and Tragardh, G. (1993) The effect of protein fouling in microfiltration and ultrafiltration on permeate flux, protein retention and selectivity: A literature review. Desalination, 91: 65-108.
20. Matthiasson, E. (1983) The role of macromolecular adsorption in fouling of ultrafiltration membranes. J. Membr. Sci., 16: 23-36.
21. Ho, C.C. and Zydney, A.L. (1999) Effect of membrane morphology on the initial rate of protein fouling during microfiltration. J. Membr. Sci., 155: 261-275.
22. Ho, C.C. and Zydney, A.L. (2000) A combined pore blockage and cake filtration model fro protein fouling during microfiltration. J. Colloid Interface Sci., 232: 389-399.
23. Nath, A. and Chauhan, A. (2005) Neurology of AIDS; Gendelman, H.E., Grant, I., Everall, I.P., Lipton, S. and Swindells (eds.); Oxford University Press: USA.
24. Ma, M. and Nath, A. (1997) Molecular determinants for cellular uptake of Tat protein of human immunodeficiency virus type 1 in brain cells. J. Virol., 71: 2495-2499.
25. Stauber, R.H. and Pavlakis, G.N. (1998) Intracellular trafficking and interactions of the HIV-1 Tat protein. Virology, 252: 126-136.
26. Kuan-Teh, Jeang, Xiao, H., and Elizabeth, A.R. (1999) Multifaceted activities of the HIV-1 transactivator of transcription, tat. J. Biol. Chem., 274: 28837-28840.
27. Ruben, S., Perkins, A., Purcell, R., Joung, K., Sia, R., Burghoff, R., Haseltine, W.A., and Rosen, C.A. (1989) Structural and functional characterization of human immunodeficiency virus tat protein. J. Virol., 63: 1-8.
28. Conant, K., Ma, M., Nath, A., and Major, E.O. (1996) Extracellular human immunodeficiency virus type 1 Tat protein is associated with an increase in both NF-kB binding and protein kinase C activity in primary human astrocytes. J. Virol., 70: 1384-1389.
29. Bradford, M.M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal, Biochem., 72: 248-254.
30. Green, N.M. (1965) A spectrophotometric assay for avidin and biotin based on binding of dyes by avidin. Biochem. J., 94: 23c-24c.
31. Components of avidin-biotin technology, Avidin-Biotin Chemistry: A Handbook, Pierce Biotechnology.
32. Mulder, M. (1991) Basic Principles of Membrane Technology; Kluwer Academic Publishers: Netherlands.