Direct purification and immobilization of recombinant hyaluronan lyase from unclarified feedstock using immobilized metal affinity magnetite for oligo-hyaluronan preparation

Biochemical Engineering Journal

Volumn 37, Issue 1, 2007, Pages 108-115

  Yang, Pei Fen ^a   Lee, Cheng Kang ^a  

^a NATIONAL TAIWAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Taiwan)

Author keywords

Hyaluronan lyase; Hyaluronic acid; Immobilization; Magnetite; Metal chelator; Oligosaccharides

Indexed keywords

CONCENTRATION (PROCESS); ENZYME IMMOBILIZATION; MOLAR MASS; MOLECULAR WEIGHT; OLIGOSACCHARIDES;

HYALURONAN LYASE; HYALURONIC ACID; IMMOBILIZED METAL AFFINITY MAGNETITE (IMAM); METAL CHELATOR;

MAGNETITE;

HYALURONIC ACID; LYASE; MAGNETITE; RECOMBINANT ENZYME;

ARTICLE; BATCH REACTOR; CELL LYSATE; CHELATION; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ESCHERICHIA COLI; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL;

EID: 35148888715     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2007.04.001     Document Type: Article

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