Improved production of p-hydroxycinnamic acid from tyrosine using a novel thermostable phenylalanine/tyrosine ammonia lyase enzyme

Enzyme and Microbial Technology

Volumn 42, Issue 1, 2007, Pages 58-64

  Xue, Zhixiong ^a   McCluskey, Michael ^a   Cantera, Keith ^a   Ben Bassat, Arie ^a   Sariaslani, F Sima ^a   Huang, Lixuan ^a  

^a DUPONT COMPANY   (United States)

Author keywords

Arabinose inducible promoter (araB); pHCA); Phenylalanine ammonia lyase (PAL); Protein expression; Thermostable enzyme; Trans p hydroxycinnamic acid (p coumarate; Tyrosine ammonia lyase (TAL)

Indexed keywords

ARABINOSE-INDUCIBLE PROMOTER (ARAB); PHENYLALANINE AMMONIA LYASE (PAL); PROTEIN EXPRESSION; TYROSINE AMMONIA LYASE (TAL);

GENE EXPRESSION; INDUSTRIAL CHEMICALS; PROTEINS; YEAST;

ENZYME ACTIVITY;

ARABINOSE; LYASE; PARA COUMARIC ACID; PHENYLALANINE AMMONIA LYASE; TYROSINE; TYROSINE AMMONIA LYASE; UNCLASSIFIED DRUG;

ARTICLE; BIOTRANSFORMATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME INDUCTION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SYNTHESIS; MOLECULAR CLONING; NONHUMAN; PHANEROCHAETE; PROTEIN EXPRESSION; REACTION ANALYSIS; RHODOTORULA GLUTINIS; SOLUBILITY; THERMOSTABILITY;

FUNGI; PHANEROCHAETE CHRYSOSPORIUM; RHODOTORULA GLUTINIS;

EID: 35148882050     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2007.07.025     Document Type: Article

References (20)

1. Frost J.W., and Draths K.M. Biocatalytic synthesis of aromatics from d-glucose: renewable microbial sources of aromatic compounds. Annu. Rev. Microbiol. 49 (1995) 557-579
2. Frost J.W., and Lievense J. Prospects for biocatalytic synthesis of aromatics in the 21st century. New J. Chem. 18 (1994) 341-348
3. Draths, KM, Frost JW. In: Anastas, PT, Williamson, TC, editors, Green chemistry. 1998, p. 150-165.
4. Gibson J.M., Thomas P.S., Thomas J.D., Barker J.l., Chandran S.S., Harrup M.K., et al. Benzene-free synthesis of phenol. Angew. Chem., Int. Ed. 40 (2001) 1945-1948
5. Dong-Eun C., Heung-Chae J., Joon-Shick R., and Jae-Gu P. Homofermentative production of d- or l-lactate in metabolically engineered Escherichia coli RR1. Appl. Environ. Microbiol. 65 (1999) 1384-1389
6. Vallino J.J., and Stephanopoulos G. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol. Bioeng. 1 (1993) 633-646
7. Altaras N.E., and Cameron D.C. Metabolic engineering of a 1.2-Propanediol pathway in Escherichia coli. Appl. Environ. Microbiol. 65 (1999) 1180-1185
8. Nakamura C.E., and Whited G.M. Metabolic engineering for the microbial production of 1,3-propanediol. Curr. Op. Biotechnol. 14 (2003) 454-549
9. Mee-Jung H., Sun Y.S., and Yup L.S. Proteome analysis of metabolically engineered Escherichia coli producing poly(3-hydroxybutyrate). J. Bacteriol. 183 (2001) 301-308
10. Priefert H., Rabenhorst J., and Steinbuchel A. Biotechnological production of vanillin. Appl. Microbiol. Biotechnol. 56 (2001) 296-314
11. Koukol J., and Conn E.E. The metabolism of aromatic compounds in higher plants. IV. Purification and properties of the phenylalanine deaminase of Hordeum vulgare. J. Biol. Chem. 236 (1961) 2692-2698
12. Parkhurst J., Rodney H., and Daniel S. Yeast phenylalanine ammonia-lyase. Properties of the enzyme from Sporobolomyces pararoseus and its catalytic site. Arch. Biochem. Biophys. 152 (1972) 597-605
13. Vannelli T., Qi W.W., Sweigard J., Gatenby A.A., and Sariaslani F.S. Production of p-hydroxycinnamic acid from glucose in Saccharomyces cerevisiae and Escherichia coli by expression of heterologous genes from plants and fungi. Metab. Eng. 9 (2007) 142-151
14. Vannelli T., Xue Z., Breinig S., Qi W.W., and Sariaslani F.S. Functional expression in Escherichia coli of the tyrosine-inducible tyrosine ammonia-lyase enzyme from yeast Trichosporon cutaneum for production of p-hydroxycinnamic acid. Enzyme Microbiol. Technol. 41 (2007) 413-422
15. Abell C.W., and Shen R.S. Phenylalanine ammonia-lyase from yeast Rhodotorula glutinis. Methods Enzymol. 142 (1987) 242-248
16. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
17. Martinez D., Larrondo L.F., Putnam N., Gelpke M.D., Huang K., Chapman J., et al. Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78. Nature Biotechnol. 22 (2004) 695-700
18. Hitchcock D.I. The solubility of tyrosine in acid or in alkali. J. Gen. Physiol. 6 (1924) 747-757
19. Lamar R.T., Main L.M., Dietrich D.M., and Glaser J.A. In: Kral D.M. (Ed). Screening of fungi for soil remediation potential. Bioremediation of contaminated soils (1999), American Society of Agronomy 438-456
20. Hermes J.D., Weiss P.M., and Cleland W.W. Use of Nitrogen-15 and deuterium isotope effects to determine the chemical mechanism of phenylalanine ammonia lyase. Biochemistry 24 (1985) 2959-2967