Preservation of RNA for functional analysis of separated alleles in yeast: Comparison of snap-frozen and RNALater® solid tissue storage methods

Clinical Chemistry and Laboratory Medicine

Volumn 45, Issue 10, 2007, Pages 1283-1287

  Dekairelle, Anne France ^a   Van Der Vorst, Sébastien ^a   Tombal, Bertrand ^b   Gala, Jean Luc ^a,c,d  

^a UNIVERSITÉ CATHOLIQUE DE LOUVAIN   (Belgium)

^b CLINIQUES UNIVERSITAIRES SAINT LUC   (Belgium)

^c Defence Laboratories Department   (Belgium)

^d Centre for Applied Molecular Technology   (Belgium)

Author keywords

Functional analysis of separated alleles in yeast (FASAY); RNA preservation; RNALater ; Snap frozen

Indexed keywords

LIQUID NITROGEN; PROTEIN P53; RNA;

ALLELE; ARTICLE; BLADDER CARCINOMA; CONTROLLED STUDY; HUMAN; HUMAN TISSUE; MISSENSE MUTATION; NONHUMAN; PRESERVATION; PRIORITY JOURNAL; RNA DEGRADATION; RNA PROCESSING; ROOM TEMPERATURE; TISSUE PRESERVATION; TRANSITIONAL CELL CARCINOMA; TUMOR BIOPSY; YEAST;

ALLELES; CARCINOMA, TRANSITIONAL CELL; CRYOPRESERVATION; CRYOPROTECTIVE AGENTS; GENE EXPRESSION PROFILING; HUMANS; NITROGEN; RNA; RNA STABILITY; RNA, MESSENGER; TIME FACTORS; TISSUE PRESERVATION; URINARY BLADDER NEOPLASMS; YEASTS;

EID: 35048890937     PISSN: 14346621     EISSN: 14374331     Source Type: Journal    
DOI: 10.1515/CCLM.2007.281     Document Type: Article

References (20)

1. Dekairelle AF, Tombal B, Cosyns JP, Gala JL. Assessment of the transcriptional activity of p53 improves the prediction of recurrence in superficial transitional cell carcinoma of the bladder. Clin Cancer Res 2005;11:4724-32.
2. Flaman JM, Frebourg T, Moreau V, Charbonnier F, Martin C, Chappuis P, et al. A simple p53 functional assay for screening cell lines, blood, and tumors. Proc Natl Acad Sci USA 1995;92:3963-7.
3. Grotzer MA, Patti R, Geoerger B, Eggert A, Chou TT, Phillips PC. Biological stability of RNA isolated from RNALater-treated brain tumor and neuroblastoma xenografts. Med Pediatr Oncol 2000;34:438-42.
4. Florell SR, Coffin CM, Holden JA, Zimmermann JW, Gerwels JW, Summers BK, et al. Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol. Mod Pathol 2001;14:116-28.
5. Ellis M, Davis N, Coop A, Liu M, Schumaker L, Lee RY, Srikanchana R, et al. Development and validation of a method for using breast core needle biopsies for gene expression microarray analyses. Clin Cancer Res 2002;8:1155-66.
6. Rodrigo MC, Martin DS, Redetzke RA, Eyster KM. A method for the extraction of high-quality RNA and protein from single small samples of arteries and veins preserved in RNALater. J Pharmacol Toxicol Methods 2002;47:87-92.
7. Mutter GL, Zahrieh D, Liu C, Neuberg D, Finkelstein D, Baker HE, et al. Comparison of frozen and RNALater solid tissue storage methods for use in RNA expression microarrays. BMC Genomics 2004;5:88.
8. Chowdary D, Lathrop J, Skelton J, Curtin K, Briggs T, Zhang Y, et al. Prognostic gene expression signatures can be measured in tissues collected in RNALater preservative. J Mol Diagn 2006;8:31-9.
9. Bland JM, Altman DG. Comparing methods of measurement: why plotting difference against standard method is misleading. Lancet 1995;346:1085-7.
10. Petersen PH, Stöckl D, Blaabjerg O, Pedersen B, Birkemose E, Thienpont L, et al. Graphical interpretation of analytical data from comparison of a field method with a reference method by use of difference plots. Clin Chem 1997;43:2039-46.
11. Olivier M, Eeles R, Hollstein M, Khan MA, Harris CC, Hainaut P. The IARC TP53 database: new online mutation analysis and recommendations to users. Hum Mutat 2002;19:607-14 (version R11, October 2006).
12. Fouquet C, Antoine M, Tisserand P, Favis R, Wislez M, Commo F, et al. Rapid and sensitive p53 alteration analysis in biopsies from lung cancer patients using a functional assay and a universal oligonucleotide array: a prospective study. Clin Cancer Res 2004;10:3479-89.
13. Sakuragi N, Watari H, Ebina Y, Yamamoto R, Steiner E, Koelbl H, et al. Functional analysis of p53 gene and the prognostic impact of dominant-negative p53 mutation in endometrial cancer. Int J Cancer 2005;116:514-9.
14. Antonov J, Goldstein DR, Oberli A, Baltzer A, Pirotta M, Fleischmann A, et al. Reliable gene expression measurements from degraded RNA by quantitative real-time PCR depend on short amplicons and a proper normalization. Lab Invest 2005;85:1040-50.
15. Scicchitano MS, Dalmas DA, Bertiaux MA, Anderson SM, Turner LR, Thomas RA, et al. Preliminary comparison of quantity, quality, and microarray performance of RNA extracted from formalin-fixed, paraffin-embedded, and unfixed frozen tissue samples. J Histochem Cytochem 2006;54:1229-37.
16. Yang W, Maqsodi B, Ma Y, Bui S, Crawford KL, McMaster GK, et al. Direct quantification of gene expression in homogenates of formalin-fixed, paraffin-embedded tissues. Biotechniques 2006;40:481-6.
17. Coudry RA, Meireles SI, Stoyanova R, Cooper HS, Carpino A, Wang X, et al. Successful application of microarray technology to microdissected formalin-fixed, paraffin-embedded tissue. J Mol Diagn 2007;9:70-9.
18. Dunmire V, Wu C, Symmans WF, Zhang W. Increased yield of total RNA from fine-needle aspirates for use in expression microarray analysis. Biotechniques 2002;33:890-6.
19. Alfredson H, Lorentzon M, Backman S, Backman A, Lerner UH. cDNA-arrays and real-time quantitative PCR techniques in the investigation of chronic Achilles tendonosis. J Orthop Res 2003;21:970-5.
20. Tisserand P, Fouquet C, Marck V, Mallard C, Fabre M, Vielh P, et al. ThinPrep-processed fine-needle samples of breast are effective material for RNA- and DNA-based molecular diagnosis: application to p53 mutation analysis. Cancer 2003;99:223-32.