Site-directed mutagenesis studies of Tn5 transposase residues involved in synaptic complex formation

Journal of Bacteriology

Volumn 189, Issue 20, 2007, Pages 7436-7441

  Vaezeslami, Soheila ^a   Sterling, Rachel ^a   Reznikoff, William S ^a,b  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b MARINE BIOLOGICAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; ASPARAGINE; GENOMIC DNA; GLUTAMIC ACID; LYSINE; SERINE; TN5 TRANSPOSASE; TRANSPOSASE; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; DNA PROTEIN COMPLEX; DNA SEQUENCE; DNA TRANSPOSITION; GENE REARRANGEMENT; GENETIC ANALYSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA BINDING; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ANALYSIS; TRANSPOSON;

AMINO ACID SUBSTITUTION; DNA TRANSPOSABLE ELEMENTS; DNA, BACTERIAL; MODELS, BIOLOGICAL; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; NUCLEIC ACID CONFORMATION; PROTEIN BINDING; PROTEIN CONFORMATION; TRANSPOSASES;

SYNAPSIS;

EID: 35048883072     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.00524-07     Document Type: Article

References (31)

1. Adams, C. D., B. Schnurr, D. Skoko, J. F. Marko, and W. S. Reznikoff. 2006. Tn5 transposase loops DNA in the absence of Tn5 transposon end sequences. Mol. Microbiol. 62:1558-1568.
2. Ason, B., and W. S. Reznikoff. 2002. Mutational analysis of the base flipping event found in Tn5 transposition. J. Biol. Chem. 277:11284-11291.
3. Berg, D. E., and M. M. Howe. 1989. Mobile DNA. American Society for Microbiology, Washington, DC.
4. Bhasin, A., I. Y. Goryshin, and W. S. Reznikoff. 1999. Hairpin formation in Tn5 transposition. J. Biol. Chem. 274:37021-37029.
5. Bhasin, A., I. Y. Goryshin, M. Steiniger-White, D. York, and W. S. Reznikoff. 2000. Characterization of a Tn5 pre-cleavage synaptic complex. J. Mol. Biol. 302:49-63.
6. Bradford, M. M. 1976. Rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
7. Craig, N. L. 2002. Mobile DNA II. ASM Press, Washington, DC.
8. Curcio, M. J., and K. M. Derbyshire. 2003. The outs and ins of transposition: from mu to kangaroo. Nat. Rev. Mol. Cell Biol. 4:865-877.
9. Davies, D. R., I. Y. Goryshin, W. S. Reznikoff, and I. Rayment. 2000. Three-dimensional structure of the Tn5 synaptic complex transposition intermediate. Science 289:77-85.
10. Goryshin, I. Y., J. A Miller, Y. V. Kil, V. A. Lanzov, and W. S. Reznikoff. 1998. Tn5/IS50 target recognition. Proc. Natl. Acad. Sci. USA 95:10716-10721.
11. Goryshin, I. Y., and W. S. Reznikoff. 1998. Tn5 in vitro transposition. J. Biol. Chem. 273:7367-7374.
12. Johnson, R. C., and W. S. Reznikoff. 1983. DNA-sequences at the ends of transposon Tn5 required for transposition. Nature 304:280-282.
13. Johnson, R. C., and W. S. Reznikoff. 1984. Role of the IS50R proteins in the promotion and control of Tn5 transposition. J. Mol. Biol. 177:645-661.
14. Krebs, M. P., and W. S. Reznikoff. 1988. Use of a Tn5 derivative that creates LacZ translational fusions to obtain a transposition mutant. Gene 63:277-285.
15. Mizuuchi, K., and K. Adzuma. 1991. Inversion of the phosphate chirality at the target site of Mu-DNA strand transfer - evidence for a one-step transesterification mechanism. Cell 66:129-140.
16. Naumann, T. A., and W. S. Reznikoff. 2002. Tn5 transposase active site mutants. J. Biol. Chem. 277:17623-17629.
17. Naumann, T. A., and W. S. Reznikoff. 2002. Tn5 transposase with an altered specificity for transposon ends. J. Bacteriol. 184:233-240.
18. Naumann, T. A., and W. S. Reznikoff. 2000. Trans catalysis in Tn5 transposition. Proc. Natl. Acad. Sci. USA 97:8944-8949.
19. Peterson, G., and W. Reznikoff. 2003. Tn5 transposase active site mutations suggest position of donor backbone DNA in synaptic complex. J. Biol. Chem. 278:1904-1909.
20. Reznikoff, W. S. 1993. The Tn5 transposon. Annu. Rev. Microbiol. 47:945-963.
21. Reznikoff, W. S. 2003. Tn5 as a model for understanding DNA transposition. Mol. Microbiol. 47:1199-1206.
22. Reznikoff, W. S. 2006. Tn5 transposition: a molecular tool for studying protein structure-function. Biochem. Soc. Trans. 34:320-323.
23. Reznikoff, W. S., A. Bhasin, D. R. Davies, I. Y. Goryshin, L. A. Mahnke, T. Naumann, I. Rayment, M. Steiniger-White, and S. S. Twining. 1999. Tn5: a molecular window on transposition. Biochem. Biophys. Res. Commun. 266:729-734.
24. Steiniger, M., C. D. Adams, J. F. Marko, and W. S. Reznikoff. 2006. Defining characteristics of Tn5 transposase non-specific DNA binding. Nucleic Acids Res. 34:2820-2832.
25. Steiniger, M., J. Metzler, and W. S. Reznikoff. 2006. Mutation of Tn5 transposase beta-loop residues affects all steps of Tn5 transposition: the role of conformational changes in Tn5 transposition. Biochemistry 45:15552-15562.
26. Steiniger-White, M., A. Bhasin, S. Lovell, I. Rayment, and W. S. Reznikoff. 2002. Evidence for "unseen" transposase-DNA contacts. J. Mol. Biol. 322: 971-982.
27. Twining, S. S., I. Y. Goryshin, A. Bhasin, and W. S. Reznikoff. 2001. Functional characterization of arginine 30, lysine 40, and arginine 62 in Tn5 transposase. J. Biol. Chem. 276:23135-23143.
28. Weinreich, M. D., A. Gasch, and W. S. Reznikoff. 1994. Evidence that the cis preference of the Tn5 transposase is caused by nonproductive multimerization. Genes Dev. 8:2363-2374.
29. Weinreich, M. D., L. Mahnke-Braam, and W. S. Reznikoff. 1994. A functional-analysis of the Tn5 transposase - identification of domains required for DNA-binding and multimerization. J. Mol. Biol. 241:166-177.
30. Zhou, M., A. Bhasin, and W. S. Reznikoff. 1998. Molecular genetic analysis of transposase-end DNA sequence recognition: cooperativity of three adjacent base-pairs in specific interaction with a mutant Tn5 transposase. J. Mol. Biol. 276:913-925.
31. Zhou, M., and W. S. Reznikoff. 1997. Tn5 transposase mutants that alter DNA binding specificity. J. Mol. Biol. 271:362-373.