Rapid maturation of glycoprotein hormone free α-subunit (GPHα) and GPHαα homodimers

Molecular Endocrinology

Volumn 21, Issue 10, 2007, Pages 2551-2564

  Krause, Jean Michel ^a   Berger, Peter ^b   Roig, Jordi ^a   Singh, Vinod ^a,c   Merz, Wolfgang E ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

^c NORTH EASTERN HILL UNIVERSITY   (India)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCAN DERIVATIVE; HOMODIMER; MONOMER;

ALPHA CHAIN; ARTICLE; CELL METABOLISM; CONTROLLED STUDY; GLYCOSYLATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN METABOLISM; PROTEIN PROCESSING; PROTEOMICS;

CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; DIMERIZATION; GLYCOPROTEIN HORMONES, ALPHA SUBUNIT; GLYCOSYLATION; HELA CELLS; HUMANS; PROTEIN CONFORMATION; PROTEIN ISOFORMS;

EID: 34948877981     PISSN: 08888809     EISSN: None     Source Type: Journal    
DOI: 10.1210/me.2007-0051     Document Type: Article

References (70)

1. Hayashi H, Hayashi T, Hanaoka Y 1992 Amphibian lutropin and follitropin from the bullfrog Rana catesbeiana. Complete amino acid sequence of the α subunit. Eur J Biochem 203:185-191
2. Curry Jr TE, Dean DD, Sanders SL, Pedigo NG, Jones PB 1989 The role of ovarian proteases and their inhibitors in ovulation. Steroids 54:501-521
3. Braunstein GD, Vaitukaitis JL, Carbone PP, Ross GT 1973 Ectopic production of human chorionic gonadotrophin by neoplasms. Ann Intern Med 78:39-45
4. Saller B, Clara R, Spottl G, Siddle K, Mann K 1990 Testicular cancer secretes intact human choriogonadotropin (hCG) and its free β-subunit: evidence that hCG (+hCG-β) assays are the most reliable in diagnosis and follow-up. Clin Chem 36:234-239
5. Beastall GH, Cook B, Rustin GJ, Jennings J 1991 A review of the role of established tumour markers. Ann Clin Biochem 28:5-18
6. Madersbacher S, Klieber R, Mann K, Marth C, Tabarelli M, Wick G, Berger P 1992 Free α-subunit, free β-subunit of human chorionic gonadotropin (hCG), and intact hCG in sera of healthy individuals and testicular cancer patients. Clin Chem 38:370-376
7. Dirnhofer S, Freund M, Rogatsch H, Krabichler S, Berger P 2000 Selective expression of trophoblastic hormones by lung carcinoma: neurendocrine tumors exclusively produce human chorionic gonadotropin α-subunit (hCGα). Hum Pathol 31:966-972
8. Darnell RB, Boime I 1985 Differential expression of the human gonadotropin α gene in ectopic and eutopic cells. Mol Cell Biol 5:3157-3167
9. Cosgrove DE, Campain JA, Cox GS 1989 Chorionic gonadotropin synthesis by human tumor cell lines: examination of subunit accumulation, steady-state levels of mRNA, and gene structure. Biochim Biophys Acta 1007:44-54
10. Berger P, Gruschwitz M, Spoettl G, Dirnhofer S, Madersbacher S, Gerth R, Merz WE, Plas E, Sampson N 2007 Human chorionic gonadotropin (hCG) in the male reproductive tract. Mol Cell Endorinol 260-262:190-196
11. Peghini PL, Annibale B, Azzoni C, Milione M, Corleto VD, Gibril F, Venzon DJ, Delle-Fave G, Bordi C, Jensen RT 2002 Effect of chronic hypergastrinemia on human enterochromaffin-like cells: insights from patients with sporadic gastrinomas. Gastroenterology 123:68-85
12. Blithe DL, Richards RG, Skarulis MC 1991 Free α molecules from pregnancy stimulate secretion of prolactin from human decidual cells: a novel function for free α in pregnancy. Endocrinology 129:2257-2259
13. Nemansky M, Moy E, Lyons CD, Yu I, Blithe DL 1998 Human endometrial stromal cells generate uncombined α-subunit from human chorionic gonadotropin, which can synergize with progesterone to induce decidualization. J Clin Endocrinol Metab 83:575-581
14. Van-Bael A, Denef C 1996 Evidence for a trophic action of the glycoprotein hormone α-subunit in rat pituitary. J Neuroendocrinol 8:99-102
15. Begeot M, Hemming FJ, Dubois PM, Combarnous Y, Dubois MP, Aubert ML 1984 Induction of pituitary lactotrope differentiation by luteinizing hormone α subunit. Science 226:566-568
16. Rumpold H, Mascher K, Untergasser G, Plas E, Hermann M, Berger P v Trans-differentiation of prostatic stromal cells leads to decreased glycoprotein hormone α production. J Clin Endocrinol Metab 87:5297-5303
17. Cole LA, Perini F, Birken S, Ruddon RW 1984 An oligosaccharide of the O-linked type distinguishes the free from the combined form of hCG α subunit. Biochem Biophys Res Commun 122:1260-1267
18. Kobata A, Takeuchi M Structure, pathology and function of the N-linked sugar chains of human chorionic gonadotropin 1999. Biochim Biophys Acta 1455:315-326
19. Beebe JS, Krzesicki RF, Norton SE, Perini F, Peters BP, Ruddon RW 1989 Identification and characterization of subpopulations of the free α-subunit that vary in their ability to combine with chorionic gonadotropin-β. Endocrinology 124:1613-1624
20. Thotakura NR, Blithe DL 1995 Glycoprotein hormones: glycobiology of gonadotrophins, thyrotrophin and free α subunit. Glycobiology 5:3-10
21. Matzuk MM, Boime I 1988 The role of the asparagine-linked oligosaccharides of the α subunit in the secretion and assembly of human chorionic gonadotrophin. J Cell Biol 106:1049-1059
22. Merz WE 1988 Evidence for impaired subunit interaction in chemically deglycosylated human choriogonadotropin. Biochem Biophys Res Commun 156:1271-1278
23. Matzuk MM, Keene JL, Boime I 1989 Site specificity of the chorionic gonadotropin N-linked oligosaccharides in signal transduction. J Biol Chem 264:2409-2414
24. Erbel PJ, Karimi-Nejad Y, van-Kuik JA, Boelens R, Kamerling JP, Vliegenthart JF 2000 Effects of the N-linked glycans on the 3D structure of the free α-subunit of human chorionic gonadotropin. Biochemistry 39:6012-6021
25. Erbel PJ, Haseley SR, Kamerling JP, Vliegenthart JF 2002 Studies on the relevance of the glycan at Asn-52 of the α-subunit of human chorionic gonadotropin in the αβ dimmer. Biochem J 364:485-495
26. Endo Y, Yamashita K, Tachibana Y, Tojo S, Kobata A 1979 Structures of the asparagine-linked sugar chains of human chorionic gonadotropin. J Biochem Tokyo 85:669-679
27. Kessler MJ, Reddy MS, Shah RH, Bahl OP 1979 Structures of N-glycosidic carbohydrate units of human chorionic gonadotropin. J Biol Chem 254:7901-7908
28. Xing Y, Williams C, Campbell RK, Cook S, Knoppers M, Addona T, Altarocca V, Moyle WR 2001 Threading of a glycosylated protein loop through a protein hole: implications for combination of human chorionic gonadotropin subunits. Protein Sci 10:226-235
29. Lapthorn AJ, Harris DC, Littlejohn A, Lustbader JW, Canfield RE, Machin KJ, Morgan FJ, Isaacs NW 1994 Crystal structure of human chorionic gonadotropin. Nature 369:455-461
30. Wu H, Lustbader JW, Liu Y, Canfield RE, Hendrickson WA1994 Structure of human chorionic gonadotropin at 2.6 A resolution from MAD analysis of the selenomethionyl protein. Structure 2:545-558
31. De-Beer T, Van-Zuylen CW, Leeflang BR, Hard K, Boelens R, Kaptein R, Kamerling JP, Vliegenthart JF 1996 NMR studies of the free α subunit of human chorionic gonadotropin. Structural influences of N-glycosylation and the β subunit on the conformation of the α subunit. Eur J Biochem 241:229-242
32. Erbel PJ, Karimi-Nejad Y, De-Beer T, Boelens R, Kamerling JP, Vliegenthart JF 1999 Solution structure of the α-subunit of human chorionic gonadotropin. Eur J Biochem 260:490-498
33. Weller CT, Lustbader J, Seshadri K, Brown JM, Chadwick CA, Kolthoff CE, Ramnarain S, Pollak S, Canfield R, Homans SW 1996 Structural and conformational analysis of glycan moieties in situ on isotopically 13C, 15N-enriched recombinant human chorionic gonadotropin. Biochemistry 35:8815-8823
34. Kouwijzer ML, Berendsen HJ, Grootenhuis PD 1999 Computer simulations of the dynamics of human choriogonadotropin and its α subunit. Proteins 37:668-682
35. Van-Zuylen CW, De-Beer T, Rademaker GJ, Haverkamp J, Thomas-Oates JE, Hard K, Kamerling JP, Vliegenthart JF 1995 Site-specific and complete enzymic deglycosylation of the native human chorionic gonadotropin α-subunit. Eur J Biochem 231:754-760
36. van-Zuylen CW, de-Beer T, Leeflang BR, Boelens R, Kaptein R, Kamerling JP, Vliegenthart JF 1998 Mobilities of the inner three core residues and the Man(α 1-6) branch of the glycan at Asn78 of the α-subunit of human chorionic gonadotropin are restricted by the protein. Biochemistry 37:1933-1940
37. Roig J, Krause JM, Berger P, Merz WE 2007 Time-dependant folding of immunological epitopes of the human chorionic gonadotropin β-subunit. Mol Cell Endocrinol 260-262:12-22
38. Hebert DN, Garman SC, Molinari M 2005 The glycan code of the endoplasmic reticulum: asparagine-linked carbohydrates as protein maturation and quality-control tags. Trends Cell Biol 15:364-370
39. Daniels R, Kurowski B, Johnson AE, Hebert DN 2003 N-linked glycans direct the cotranslational folding pathway of influenza hemagglutinin. Mol Cell 11:79-90
40. Allen S, Naim HY, Bulleid NJ 1995 Intracellular folding of tissue-type plasminogen activator. Effects of disulfide bond formation on N-linked glycosylation and secretion. J Biol Chem 270:4797-4804
41. McDonald NQ, Hendrickson WA 1993 A structural superfamily of growth factors containing a cystine knot motif. Cell 73:421-424
42. Isaacs NW 1995 Cystine knots. Curr Opin Struct Biol 5:391-395
43. Vitt UA, Hsu SY, Hsueh AJ 2001 Evolution and classification of cystine knot-containing hormones and related extracellular signaling molecules. Mol Endocrinol 15:681-694
44. Furuhashi M, Ando H, Bielinska M, Pixley MR, Shikone T, Hsueh AJ, Boime I 1994 Mutagenesis of cysteine residues in the human gonadotropin α subunit. Roles of individual disulfide bonds in secretion, assembly, and biologic activity. J Biol Chem 269:25543-25548
45. Darling RJ, Ruddon RW, Perini F, Bedows E 2000 Cystine knot mutations affect the folding of the glycoprotein hormone α-subunit. Differential secretion and assembly of partially folded intermediates. J Biol Chem 275:15413-15421
46. Darling RJ, Wilken JA, Ruddon RW, Bedows E 2001 Intracellular folding pathway of the cystine knot-containing glycoprotein hormone α-subunit. Biochemistry 40:577-585
47. Mishra AK, Mahale SD, Iyer KS 2003 Disulfide bonds Cys(9)-Cys(57), Cys(34)-Cys(88) and Cys(38)-Cys(90) of the β-subunit of human chorionic gonadotropin are crucial for heterodimer formation with the α-subunit: experimental evidence for the conclusions from the crystal structure of hCG. Biochim Biophys Acta 1645:49-55
48. Darling RJ, Wilken JA, Miller-Lindholm AK, Urlacher TM, Ruddon RW, Sherman SA, Bedows E 2001 Functional contributions of noncysteine residues within the cystine knots of human chorionic gonadotropin subunits. J Biol Chem 276:10692-10699
49. Hilf G, Merz WE 1989 Influence of cyclic nucleotides on the processing of the carbohydrate part of the α-subunit of human choriogonadotropin by first trimester human placenta tissue. Biochem Biophys Res Commun 159:26-33
50. Ben-Dor S, Esterman N, Rubin E, Sharon N 2004 Biases and complex patterns in the residues flanking protein N-glycosylation sites. Glycobiology 14:95-101
51. Dubuisson J, Duvet S, Meunier JC, Op-De-Beeck A, Cacan R, Wychowski C, Cocquerel L 2000 Glycosylation of the hepatitis C virus envelope protein E1 is dependent on the presence of a downstream sequence on the viral polyprotein. J Biol Chem 275:30605-30609
52. Bolt G, Kristensen C, Steenstrup TD 2005 Posttranslational N-glycosylation takes place during the normal processing of human coagulation factor VII. Glycobiology 15:541-547
53. Singh V, Merz WE 1997 In vivo reduction/re-oxidation studies of hCG-αsubunits. Exp Clin Endocrinol Diabetes 105:79
54. Butnev VY, Singh V, Nguyen VT, Bousfield GR 2002 Truncated equine LH β and asparagine(56)-deglycosylated equine LH α combine to produce a potent FSH antagonist. J Endocrinol 172:545-555
55. Kolhekar AS, Quon AS, Berard CA, Mains RE, Eipper BA 1998 Post-translational N-glycosylation of a truncated form of a peptide processing enzyme. J Biol Chem 273:23012-23018
56. Blithe DL 1990 N-linked oligosaccharides on free α interfere with its ability to combine with human chorionic gonadotropin-β subunit. J Biol Chem 265:21951-21956
57. Kawano T, Endo T, Nishimura R, Mizuochi T, Mochizuki M, Kochibe N, Kobata A 1988 Structural differences found in the sugar chains of eutopic and ectopic free α-subunits of human glycoprotein hormone. Arch Biochem Biophys 267:787-796
58. Blithe DL, Iles RK 1995 The role of glycosylation in regulating the glycoprotein hormone free α-subunit and free β-subunit combination in the extraembryonic coelomic fluid of early pregnancy. Endocrinology 136:903-910
59. Bell SL, Xu G, Forstner JF 2001 Role of the cystine-knot motif at the C-terminus of rat mucin protein Muc2 in dimer formation and secretion. Biochem J 357:203-209
60. Peng KC, Puett D, Brewer JM 1997 Homodimer formation by the individual subunits of bovine lutropin as determined by sedimentation equilibrium. J Mol Endocrinol 18:259-265
61. Butler SA, Laidler P, Porter JR, Kicman AT, Chard T, Cowan DA, Iles RK 1999 The β-subunit of human chorionic gonadotrophin exists as a homodimer. J Mol Endocrinol 22:185-192
62. Lobel L, Pollak S, Wang S, Chaney M, Lustbader JW 1999 Expression and characterization of recombinant β-subunit hCG homodimer. Endocrine 10:261-270
63. Berger P, Schwarz S, Spottl G, Wick G, Mann K 1993 Variants of human chorionic gonadotropin from pregnant women and tumor patients recognized by monoclonal antibodies. J Clin Endocrinol Metab 77:347-351
64. Berger P, Bidart JM, Delves PS, Dirnhofer S, Hoermann R, Isaacs N, Jackson A, Klonisch T, Lapthorn A, Lund T, Mann K, Roitt I, Schwarz S, Wick G 1996 Immunochemical mapping of gonadotropins. Mol Cell Endocrinol 125:33-43
65. Butler SA, Iles RK 2004 The free monomeric β subunit of human chorionic gonadotrophin (hCG β) and the recently identified homodimeric β-β subunit (hCG β β) both have autocrine growth effects. Tumour Biol 25:18-23
66. Haas MJ, Cosgrove DE, Xiong W, Cox GS 1999 Sodium butyrate-mediated induction of the glycoprotein hormone α-subunit gene: requirement for continued protein synthesis, identification of a butyrate-responsive element, and inhibition of promoter activation by 2-deoxyglucose. J Cell Biochem 74:242-263
67. Singh V, Merz WE 2000 Disulfide bond formation is not required for human chorionic gonadotropin subunit association. Studies with dithiothreitol in JEG-3 cells. J Biol Chem 275:11765-11770
68. Berger P, Panmoung W, Khaschabi D, Mayregger B, Wick G 1988 Antigenic features of human follicle stimulating hormone delineated by monoclonal antibodies and construction of an immunoradiomometric assay. Endocrinology 123:2351-2359
69. Berger P, Klieber R, Panmoung W, Madersbacher S, Wolf H, Wick G 1990 Monoclonal antibodies against the free subunits of human chorionic gonadotrophin. J Endocrinol 125:301-309
70. Furuhashi M, Suganuma N 2003 Effect of additional N-glycosylation signal in the N-terminal region of intracellular function of the gonadotropin α-subunit. Endocr J 50: 245-253