A method for activity staining after native polyacrylamide gel electrophoresis using a coupled enzyme assay and fluorescence detection: Application to the analysis of several glycolytic enzymes

Analytical Biochemistry

Volumn 300, Issue 1, 2002, Pages 94-99

  Rivoal, Jean ^a   Smith, Christopher R ^b   Moraes, Trevor F ^b   Turpin, David H ^c   Plaxton, William C ^b  

^a UNIVERSITY OF MANITOBA   (Canada)

^b QUEEN S UNIVERSITY   (Canada)

^c UNIVERSITY OF VICTORIA   (Canada)

Author keywords

Activity stain; Coupled enzyme assay; Fluorescence detection; Isozyme; Native polyacrylamide gel electrophoresis; Phosphoenolpyruvate carboxylase; PPi dependent phosphofructokinase; Pyruvate kinase

Indexed keywords

ELECTROPHORESIS; FLUORESCENCE;

ENZYME ASSAYS; FLUORESCENCE DETECTION; ISOZYME; PHOSPHOENOLPYRUVATE CARBOXYLASE; PHOSPHOFRUCTOKINASE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PYRUVATE KINASE;

ENZYME ACTIVITY;

6 PHOSPHOFRUCTOKINASE; PHOSPHOENOLPYRUVATE CARBOXYLASE; PYRUVATE KINASE;

ARTICLE; ENZYME ACTIVITY; ENZYME ASSAY; FLUORESCENCE; GLYCOLYSIS; NONHUMAN; PLANT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

EID: 0036147575     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5445     Document Type: Article

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