An efficient purification and fractionation of genomic DNA from soil by modified troughing method

Letters in Applied Microbiology

Volumn 45, Issue 4, 2007, Pages 387-391

  Harnpicharnchai, P ^a,b   Thongaram, T ^a   Sriprang, R ^a   Champreda, V ^a   Tanapongpipat, S ^a   Eurwilaichitr, L ^a  

^a NATIONAL SCIENCE AND TECHNOLOGY DEVELOPMENT AGENCY   (Thailand)

^b 111 Thailand Science Park   (Thailand)

Author keywords

DNA purification; DNA size fractionation; Genomic DNA from soil; Humic acids; Metagenomic library

Indexed keywords

CLONING; ELECTROPHORESIS; GENES; PURIFICATION; SOILS;

DNA PURIFICATION; DNA SIZE FRACTIONATION; GENOMIC DNA; GENOMIC DNA FROM SOIL; HUMIC ACID; METAGENOMIC LIBRARIES; MICROBIOTAS; NATURAL ENVIRONMENTS; SIZE FRACTIONATION; SIZE RANGES;

DNA;

GENOMIC DNA; MACROGOL 8000;

DNA; FRACTIONATION; GRAIN SIZE AND SHAPE; PURIFICATION; SOILS;

DNA; ELECTROKINESIS; FRACTIONATION; GENOMICS; TROUGH;

ARTICLE; BIOTA; DNA EXTRACTION; FRACTIONATION; GEL ELECTROPHORESIS; GENE LIBRARY; INTESTINE; ISOPTERA; MICROBIAL ACTIVITY; NONHUMAN; PURIFICATION; SOIL;

ISOPTERA;

EID: 34848920068     PISSN: 02668254     EISSN: 1472765X     Source Type: Journal    
DOI: 10.1111/j.1472-765X.2007.02200.x     Document Type: Article

References (12)

1. Handelsman, J. (2004) Metagenomics: application of genomics to uncultured microorganisms. Microbiol Mol Biol Rev 68, 669 685.
2. Handelsman, J., Rondon, M.R., Brady, S.F., Clardy, J. Goodman, R.M. (1998) Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol 5, R245 R249.
3. Harry, M., Gambier, B., Bourezgui, Y. Garnier-Sillam, E. (1999) Evaluation of purification procedures for DNA extracted from organic rich samples: interference with humic substances. Analusis 27, 439 442.
4. Hugenholtz, P., Goebel, B.M. Pace, N.R. (1998) Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity. J Bacterial 180, 4765 4774.
5. Kauffmann, I.M., Schmitt, J. Schmid, R.D. (2004) DNA isolation from soil samples for cloning in different hosts. Appl Microbiol Biotechnol 64, 665 670.
6. Kim, T.K. Fuerst, J.A. (2006) Diversity of polyketide synthase genes from bacteria associated with the marine sponge Pseudoceratina clavata: culture-dependent and culture-independent approaches. Environ Microbiol 8, 1460 1470.
7. Miller, D.N., Bryant, J.E., Madsen, E.L. Ghiorse, W.C. (1999) Evaluation and optimization of DNA extraction and purification procedures for soil and sediment samples. Appl Environ Microbiol 65, 4715 4724.
8. Strong, S.J., Ohta, Y., Litman, G.W. Ameniya, C.T. (1997) Marked improvement of PAC and BAC cloning is achieved using electroelution of pulsed-field gel-separated partial digests of genomic DNA. Nucleic Acid Res 25, 3959 3961.
9. Yeates, C., Gillings, M.R., Davison, A.D., Altavilla, N. Veal, D.A. (1997) PCR amplification of crude microbial DNA extracted from soil. Lett Appl Microbiol 25, 303 307.
10. Yun, J., Kang, S., Park, S., Yoon, H., Kim, M.J., Heu, S. Ryu, S. (2004) Characterization of a novel amylolytic enzyme encoded by a gene from a soil-derived metagenomic library. Appl Environ Microbiol 70, 7229 7235.
11. Zhen, L. Swank, R.T. (1993) A simple and high yield method for recovering DNA from agarose gels. BioTechniques 14, 894 898.
12. Zhou, J., Bruns, M.A. Tiedje, J.M. (1996) DNA recovery from soils of diverse composition. Appl Environ Microbiol 62, 316 322.