Cross-linked forms of the isolated N-terminal domain of the lethal factor are potent inhibitors of anthrax toxin

Infection and Immunity

Volumn 75, Issue 10, 2007, Pages 5052-5058

  Juris, Stephen J ^a,c   Melnyk, Roman A ^a   Bolcome III, Robert E ^b   Chan, Joanne ^b   Collier, R John ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

^c CENTRAL MICHIGAN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRAX TOXIN; BACTERIAL ANTIGEN; CYSTEINE; DIMER; EDEMA FACTOR; MONOMER; POLYPEPTIDE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BACILLUS ANTHRACIS; CARBOXY TERMINAL SEQUENCE; CELL SURFACE; COMPLEX FORMATION; CONTROLLED STUDY; CROSS LINKING; CYTOSOL; EMBRYO; ENDOSOME; ION CONDUCTANCE; LETHAL GENE; MAMMAL CELL; MEMBRANE TRANSPORT; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DOMAIN; PROTEIN TRANSPORT; ZEBRA FISH;

ANIMALS; ANTHRAX; ANTIGENS, BACTERIAL; ANTITOXINS; BACTERIAL TOXINS; CHO CELLS; CRICETINAE; CRICETULUS; FISH DISEASES; PROTEIN STRUCTURE, TERTIARY; ZEBRAFISH;

EID: 34848903583     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.00490-07     Document Type: Article

References (28)

1. Benson, E. L., P. D. Huynh, A. Finkelstein, and R. J. Collier. 1998. Identification of residues lining the anthrax protective antigen channel. Biochemistry 37:3941-3948.
2. Bernard, A., and M. Payton. 1995. Production of recombinant proteins, p. 5.3.1-5.3.18. In J. E. Coligan, B. M. Dunn, H. L. Plough, D. W. Speicher, and P. T. Wingfield (ed.), Current protocols in protein science. John Wiley and Sons, Inc., New York, NY.
3. Bradley, K. A., J. Mogridge, M. Mourez, R. J. Collier, and J. A. Young. 2001. Identification of the cellular receptor for anthrax toxin. Nature 414:225-229.
4. Chan, J., and F. C. Serluca. 2004. Chemical approaches to angiogenesis. Methods Cell Biol. 76:475-487.
5. Cunningham, K., D. B. Lacy, J. Mogridge, and R. J. Collier. 2002. Mapping the lethal factor and edema factor binding sites on oligomeric anthrax protective antigen. Proc. Natl. Acad. Sci. USA 99:7049-7053.
6. Duesbery, N. S., C. P. Webb, S. H. Leppla, V. M. Gordon, K. R. Klimpel, et al. 1998. Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor. Science 280:734-737.
7. Krantz, B. A., A. D. Trivedi, K. Cunningham, K. A. Christensen, and R. J. Collier. 2004. Acid-induced unfolding of the amino-terminal domains of the lethal and edema factors of anthrax toxin. J. Mol. Biol. 344:739-756.
8. Krantz, B. A., R. A. Melnyk, S. Zhang, S. J. Juris, D. B. Lacy, et al. 2005. A phenylalanine clamp catalyzes protein translocation through the anthrax toxin pore. Science 309:777-781.
9. Lacy, D. B., M. Mourez, A. Fouassier, and R. J. Collier. 2002. Mapping the anthrax protective antigen binding site on the lethal and edema factors. J. Biol. Chem. 277:3006-3010.
10. Lacy, D. B., H. C. Lin, R. A. Melnyk, O. Schueler-Furman, L. Reither, et al. 2005. A model of anthrax toxin lethal factor bound to protective antigen. Proc. Natl. Acad. Sci. USA 102:16409-16414.
11. Lawson, N. D., and B. M. Weinstein. 2002. In vivo imaging of embryonic vascular development using transgenic Zebrafish. Dev. Biol. 248:307-318.
12. Leppla, S. H. 1982. Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells. Proc. Natl. Acad. Sci. USA 79:3162-3166.
13. Melnyk, R. A., and R. J. Collier. 2006. A loop network within the anthrax toxin pore positions the phenylalanine clamp in an active conformation. Proc. Natl. Acad. Sci. USA 103:9802-9807.
14. Melnyk, R. A., K. M. Hewitt, D. B. Lacy, H. C. Lin, C. R. Gessner, et al. 2006. Structural determinants for the binding of anthrax lethal factor to oligomeric protective antigen. J. Biol. Chem. 281:1630-1635.
15. Miller, C. J., J. L. Elliott, and R. J. Collier. 1999. Anthrax protective antigen: prepore-to-pore conversion. Biochemistry 38:10432-10441.
16. Milne, J. C., S. R. Blanke, P. C. Hanna, and R. J. Collier. 1995. Protective antigen-binding domain of anthrax lethal factor mediates translocation of a heterologous protein fused to its amino- or carboxy-terminus. Mol. Microbiol. 15:661-666.
17. Milne, J. C., D. Furlong, P. C. Hanna, J. S. Wall, and R. J. Collier. 1994. Anthrax protective antigen forms oligomers during intoxication of mammalian cells. J. Biol. Chem. 269:20607-20612.
18. Mogridge, J., K. Cunningham, D. B. Lacy, M. Mourez, and R. J. Collier. 2002. The lethal and edema factors of anthrax toxin bind only to oligomeric forms of the protective antigen. Proc. Natl. Acad. Sci. USA 99:7045-7048.
19. Molloy, S. S., P. A. Bresnahan, S. H. Leppla, K. R. Klimpel, and G. Thomas. 1992. Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen. J. Biol. Chem. 267:16396-16402.
20. Pellizzari, R., C. Guidi-Rontani, G. Vitale, M. Mock, and C. Montecucco. 1999. Anthrax lethal factor cleaves MKK3 in macrophages and inhibits the LPS/IFNgamma-induced release of NO and TNFalpha. FEBS Lett. 462:199-204.
21. Petosa, C., R. J. Collier, K. R. Klimpel, S. H. Leppla, and R. C. Liddington. 1997. Crystal structure of the anthrax toxin protective antigen. Nature 385:833-838.
22. Qa'dan, M., K. A. Christensen, L. Zhang, T. M. Roberts, and R. J. Collier. 2005. Membrane insertion by anthrax protective antigen in cultured cells. Mol. Cell Biol. 25:5492-5498.
23. Scobie, H. M., G. J. Rainey, K. A. Bradley, and J. A. Young. 2003. Human capillary morphogenesis protein 2 functions as an anthrax toxin receptor. Proc. Natl. Acad. Sci. USA 100:5170-5174.
24. Vitale, G., R. Pellizzari, C. Recchi, G. Napolitani, M. Mock, et al. 1998. Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine/threonine phosphorylation of MAPKs in cultured macrophages. Biochem. Biophys. Res. Commun. 248:706-711.
25. Weinstein, B. M., D. L. Stemple, W. Driever, and M. C. Fishman. 1995. Gridlock, a localized heritable vascular patterning defect in the Zebrafish. Nat. Med. 1:1143-1147.
26. Wesche, J., J. L. Elliott, P. O. Falnes, S. Olsnes, and R. J. Collier. 1998. Characterization of membrane translocation by anthrax protective antigen. Biochemistry 37:15737-15746.
27. Wigelsworth, D. J., B. A. Krantz, K. A. Christensen, D. B. Lacy, S. J. Juris, et al. 2004. Binding stoichiometry and kinetics of the interaction of a human anthrax toxin receptor, CMG2, with protective antigen. J. Biol. Chem. 279:23349-23356.
28. Zhang, S., E. Udho, Z. Wu, R. J. Collier, and A. Finkelstein. 2004. Protein translocation through anthrax toxin channels formed in planar lipid bilayers. Biophys. J. 87:3842-3849.