A real-time PCR assay for detection and quantification of Mycoplasma agalactiae DNA

Journal of Applied Microbiology

Volumn 103, Issue 4, 2007, Pages 918-923

  Lorusso, A ^a   Decaro, N ^a   Greco, G ^a   Corrente, M ^a   Fasanella, A ^b   Buonavoglia, D ^a  

^a UNIVERSITY OF BARI   (Italy)

^b ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLA PUGLIA E DELLA BASILICATA   (Italy)

Author keywords

Detection; Milk; Mycoplasma agalactiae; Quantification; Real time PCR; Sheep

Indexed keywords

DAIRY PRODUCTS; MAMMALS; POLYMERASE CHAIN REACTION;

DETECTION; DETECTION AND QUANTIFICATIONS; MILK; MILK SAMPLE; MYCOPLASMA AGALACTIAE; QUANTIFICATION; REAL TIME POLYMERASE CHAIN REACTIONS; SHEEP; SHEEP MILKS; SPECIFIC TOOL;

DNA;

BACTERIAL DNA; MEMBRANE PROTEIN;

BACTERIUM; DETECTION METHOD; DNA; MILK; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME; RUMINANT;

ARTICLE; BACTERIAL STRAIN; BACTERIUM DETECTION; BOVIDS; CONTROLLED STUDY; DNA DETERMINATION; MILK; MYCOPLASMA; MYCOPLASMA AGALACTIAE; NONHUMAN; NUCLEOTIDE SEQUENCE; PATHOGENESIS; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SENSITIVITY ANALYSIS;

MYCOPLASMA AGALACTIAE; MYCOPLASMATALES; OVIS ARIES;

EID: 34848818765     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2007.03324.x     Document Type: Article

References (26)

1. Assunção, P., Rosales, R.S., Rifatbegovic, M., Antunes, N.T., De la Fe, C., Ruiz de Galarreta, C.M. Poveda, J.B. (2006) Quantification of mycoplasmas in broth medium with sybr green-I and flow cytometry. Front Biosci 11, 492 497.
2. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A. Struhl, K. (1993) Current Protocols in Molecular Biology, vol. 1. New York : Wiley Interscience.
3. Bergonier, D., Berthelot, X. Poumarat, F. (1997) Contagious agalactia of small ruminants: current knowledge concerning epidemiology, diagnosis and control. Rev Sci Tech 16, 848 873.
4. Buonavoglia, D., Fasanella, A., Greco, G. Pratelli, A. (1999) A study on an experimental infection of sheep with Mycoplasma agalactiae. New Microbiol 22, 27 30.
5. Chavez Gonzalez, Y.R., Ros Bascunana, C., Bolske, G., Mattsson, J.G., Fernandez Molina, C. Johansson, K.E. (1995) In vitro amplification of the 16S rRNA genes from Mycoplasma bovis and Mycoplasma agalactiae by PCR. Vet Microbiol 47, 183 190.
6. DaMassa, A.J. (1995) Mycoplasma infections of goats and sheep. In Molecular and Diagnostic Procedures in Mycoplasmology, vol. II ed. Tully, J.G. Razin, S. pp. 265 273. San Diego : Academic Press.
7. DaMassa, A.J., Brooks, D.L., Holmberg, C.A. Moe, A.I. (1987) Caprine mycoplasmosis: an outbreak of mastitis and arthritis requiring the destruction of 700 goats. Vet Rec 102, 409 413.
8. DaMassa, A.J., Wakenell, S. Brooks, D.L. (1992) Mycoplasmas of goat and sheep. J Vet Diagn Invest 4, 101 113.
9. De la Fe, C., Assunção, P., Antunes, T., Rosales, R.S. Poveda, J.B. (2005) Microbiological survey for Mycoplasma spp. in a contagious endemic area. Vet J 170, 257 259.
10. Decaro, N., Elia, G., Martella, V., Desario, C., Campolo, M., Di Trani, L., Tarsitano, E., Tempesta, M. et al. (2005) A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 DNA in the feces of dogs. Vet Microbiol 105, 19 28.
11. Dedieu, L., Mady, V. Lefevre, P.C. (1995) Development of two PCR assays for the identification of mycoplasmas causing contagious agalactia. FEMS Microbiol Lett 129, 243 250.
12. Desario, C., Decaro, N., Campolo, M., Cavalli, A., Cirone, F., Elia, G., Martella, V., Lorusso, E. et al. (2005) Canine parvovirus infection: which diagnostic test for virus? J Virol Methods 121, 179 185.
13. Foddai, A., Idini, G., Fusco, M., Rosa, N., De la Fe, C., Zinellu, S., Corona, L. Tola, S. (2005) Rapid differential diagnosis of Mycoplasma agalactiae and Mycoplasma bovis based on a multiplex-PCR and a PCR-RFLP. Mol Cell Probes 19, 207 212.
14. Freundt, E.A. (1983) Culture media for classic mycoplasmas. In Methods in Mycoplasmology, vol. I ed. Razin, S. Tully, J.G. pp. 127 135. New York : Academic Press.
15. Gorton, T.S., Barnett, M.M., Gull, T., French, R.A., Lu, Z., Kutish, G.F., Adams, L.G. Geary, S.J. (2005) Development of real-time diagnostic assays specific for Mycoplasma mycoides subspecies mycoides small colony. Vet Microbiol 111, 51 58.
16. Greco, G., Corrente, M., Martella, V., Pratelli, A. Buonavoglia, D. (2001) A multiplex-PCR for the diagnosis of contagious agalactia of sheep and goats. Mol Cell Probes 15, 21 25.
17. Greco, G., Corrente, M., Buonavoglia, D., Aliberti, A. Fasanella, A. (2002) Inactivated vaccine induces protection against Mycoplasma agalactiae infection in sheep. New Microbiol 25, 17 20.
18. Heid, C.A., Stevens, J., Livak, K.J. Williams, P.M. (1996) Real time quantitative PCR. Genome Res 6, 986 994.
19. Leach, R., Costas, M. Michelmore, D.L. (1989) Relationship between Mycoplasma mycoides subsp. mycoides (large-colony strain) and M. mycoides subsp. capri, as indicated by numerical analysis of one-dimensional SDS-PAGE protein patterns. J Gen Microbiol 135, 2993 3000.
20. Mattsson, J.G., Guss, B. Johansson, K.E. (1994) The phylogeny of Mycoplasma bovis as determined by sequence analysis of the 16S rRNA gene. FEMS Microbiol Lett 115, 325 328.
21. Meynell, G.C. Meynell, E. (1970) Quantitative aspects of microbiological experiments. In Theory and Practice in Experimental Bacteriology, 2nd edn. pp. 173 196. London : Cambridge University Press.
22. Nicholas, R. (2002) Improvements in the diagnosis and control of diseases of small ruminants caused by mycoplasmas. Small Rumin Res 45, 145 149.
23. Pinnow, C.C., Butler, J.A., Sachse, K., Hotzel, H., Timms, L.L. Rosenbusch, R.F. (2001) Detection of Mycoplasma bovis in preservative-treated field milk samples. J Dairy Sci 84, 1640 1645.
24. Ross, R.F. (1993) Mycoplasma-animal pathogens. In Rapid Diagnosis of Mycoplasmas ed. Kahane, I. Adoni, A. pp 69 109. New York : Plenum Press.
25. Subramaniam, S., Bergonier, D., Poumarat, F., Capaul, S., Schalatter, Y., Nicolet, J. Frey, J. (1998) Species identification of Mycoplasma bovis and Mycoplasma agalactiae based on the uvrC genes by PCR. Mol Cell Probes 12, 161 169.
26. Tola, S., Idini, G., Manunta, D., Galleri, G., Angioi, P.P., Rocchigiani, A.M. Leori, G. (1996) Rapid and specific detection of Mycoplasma agalactiae by polymerase chain reaction. Vet Microbiol 51, 77 84.