High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli

FEBS Journal

Volumn 274, Issue 20, 2007, Pages 5382-5389

  Zocher, Georg ^a   Wiesand, Ulrich ^a   Schulz, Georg E ^a  

^a UNIVERSITY OF FREIBURG   (Germany)

Author keywords

Biosynthesis of L cysteine; Enzymatic assay; Homodimer asymmetry; Nonstandard L amino acids; X ray diffraction

Indexed keywords

BACTERIAL ENZYME; CARBOXYLIC ACID; CITRIC ACID; CYSTEINE SYNTHASE; ISOENZYME; NITROBENZOIC ACID DERIVATIVE; THIONITROBENZOIC ACID; UNCLASSIFIED DRUG;

ARTICLE; ASYMMETRIC CATALYSIS; CATALYSIS; CHEMICAL STRUCTURE; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; STRUCTURE ANALYSIS; X RAY DIFFRACTION;

BINDING SITES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; CYSTEINE SYNTHASE; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; ISOENZYMES; MODELS, MOLECULAR; PROTEIN CONFORMATION; PROTEIN SUBUNITS; PYRIDOXAL PHOSPHATE; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY;

ESCHERICHIA COLI;

EID: 34848818562     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.06063.x     Document Type: Article

References (29)

1. Cooper AJL (1983) Biochemistry of sulfur-containing amino acids. Annu Rev Biochem 52, 187 222.
2. Kredich NM (1996) Biosynthesis of cysteine. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology (Neidhard FC, ed.), pp. 514 527. ASM Press, Washington DC.
3. Borup B Ferry JG (2000) Cysteine biosynthesis in the Archaea: Methanosarcina thermophila utilizes O-acetylserine sulfhydrylase. FEMS Microbiol Lett 189, 205 210.
4. Mino K Ishikawa K (2003) Characterization of a novel thermostable O-acetylserine sulfhydrylase from Aeropyrum pernix K1. J Bacteriol 185, 2277 2284.
5. Wirtz M, Droux M Hell R (2004) O-acetylserine (thiol) lyase: an enigmatic enzyme of plant cysteine biosynthesis revisited in Arabidopsis thaliana. J Exp Bot 55, 1785 1798.
6. Claus MT, Zocher GE, Maier THP Schulz GE (2005) Structure of the O-acetylserine sulfhydrylase isoenzyme CysM from Escherichia coli. Biochemistry 44, 8620 8626.
7. Maier THP (2003) Semisynthetic production of unnatural 1-α-amino acids by metabolic engineering of the cysteine biosynthetic pathway. Nat Biotechnol 21, 422 427.
8. Watkins KJ (2001) Peptides: a boom in the making. Chem Eng News January 8, 11 15.
9. Kaldor SW, Kalish VJ, Davies IIJF, Shetty BV, Fritz JE, Appelt K, Burgess JA, Campanale KM, Chirgadze NY, Clawson DK et al. (1997) Viracept (Nelfinavir Mesylate, AG1343): a potent, orally bioavailable inhibitor of HIV-1 protease. J Med Chem 40, 3979 3985.
10. Burkhard P, Rao GSJ, Hohenester E, Schnackerz KD, Cook PF Jansonius JN (1998) Three-dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium. J Mol Biol 283, 121 133.
11. Burkhard P, Tai CH, Ristroph CM, Cook PF Jansonius JN (1999) Ligand binding induces a large conformational change in O-acetylserine sulfhydrylase from Salmonella typhimurium. J Mol Biol 291, 941 953.
12. Burkhard P, Tai CH, Jansonius JN Cook PF (2000) Identification of an allosteric anion-binding site on O-acetylserine sulfhydrylase: structure of the enzyme with chloride bound. J Mol Biol 303, 279 286.
13. Huang B, Vetting MW Roderick SL (2005) The active site of O-acetylserine sulfhydrylase is the anchor point for bienzyme complex formation with serine acetyltransferase. J Bacteriol 187, 3201 3205.
14. Schnell R, Oehlmann W, Singh M Schneider G (2007) Structural insights into catalysis and inhibition of O-acetylserine sulfhydrylase from Mycobacterium tuberculosis: crystal structures of the enzyme-α-aminoacrylate intermediate and an enzyme-inhibitor complex. J Biol Chem 282, 23 473 23 481.
15. Heine A, Canaves JM, von Delft F, Brinen LS, Dai X, Deacon AM, Elsliger MA, Eshaghi S, Floyd R, Godzik A et al. (2004) Crystal structure of O-acetylserine sulfhydrylase (TM0665) from Thermotoga maritima at 1.8 Å resolution. Proteins 56, 387 391.
16. Bonner ER, Cahoon RE, Knapke SM Jez JM (2005) Molecular basis of cysteine biosynthesis in plants. Structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana. J Biol Chem 280, 38 803 38 813.
17. Francois JA, Kumaran S Jez JM (2006) Structural basis for interaction of O-acetylserine sulfhydrylase and serine acetyltransferase in the Arabidopsis cysteine synthase complex. Plant Cell 18, 3647 3655.
18. Chattopadhyay A, Meier M, Ivaninskii S Burkhard P (2007) Structure, mechanism, and conformational dynamics of O-acetylserine sulfhydrylase from Salmonella typhimurium: comparison of A and B isozymes. Biochemistry 46, 8315 8330.
19. Tai CH, Nalabolu SR, Jacobson TM, Minter DE Cook PF (1993) Kinetic mechanisms of the A and B isozymes of O-acetylserine sulfhydrylase from Salmonella typhimurium LT-2 using the natural and alternative reactants. Biochemistry 32, 6433 6442.
20. Price NC Stevens L (1984) Fundamentals of Enzymology, p. 136. Oxford University Press, Oxford.
21. Brzovic PS, Choi WE, Borchardt D, Kaarsholm NC Dunn MF (1994) Structural asymmetry and half-site reactivity in the T to R allosteric transition of the insulin hexamer. Biochemistry 33, 13 057 13 069.
22. Kabsch W (1993) Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants. J Appl Crystallogr 26, 795 800.
23. Storoni LC, McCoy AJ Read RJ (2004) Likelihood-enhanced fast rotation functions. Acta Crystallogr D60, 432 438.
24. Perrakis A, Morris RM Lamzin VS (1999) Automated protein model building combined with iterative structure refinement. Nat Struct Biol 6, 458 463.
25. Emsley P Cowtan K (2004) COOT: model-building tools for molecular graphics. Acta Crystallogr D60, 2126 2132.
26. Murshudov GN, Vagin AA Dodson EJ (1997) Refinement of macromolecular structures by the maximum likelihood method. Acta Crystallogr D53, 240 255.
27. Painter J Merritt EA (2006) Optimal description of a protein structure in terms of multiple groups undergoing TLS motion. Acta Crystallogr D62, 439 450.
28. Lovell SC, Davis IW, Arendall WB III., de Bakker PIW, Word JM, Prisant MG, Richardson JS Richardson DC (2003) Structure validation by Cα geometry: π, ψ, and Cβ deviation. Proteins 50, 437 450.
29. Fenn TD, Ringe D Petsko GA (2003) POVScript+: a program for model and data visualization using persistence of vision ray-tracing. J Appl Crystallogr 36, 944 947.