A novel HPLC method reveals that precipitation of 2′-deoxyadenosine 5′-monophosphate with lithium perchlorate/acetone leads to base depurination

Analytical Biochemistry

Volumn 370, Issue 2, 2007, Pages 255-257

  Evers, David L ^a   Fowler, Carol B ^a   Cunningham, Robert E ^a   Mason, Jeffrey T ^a   O'Leary, Timothy J ^b  

^a ARMED FORCES INSTITUTE OF PATHOLOGY   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INORGANIC COMPOUNDS;

DEOXYADENOSINE; DEPURINATION; HPLC METHOD; LITHIUM PERCHLORATE; MONOPHOSPHATES;

LITHIUM COMPOUNDS;

ACETONE; DEOXYADENOSINE PHOSPHATE; LITHIUM; LITHIUM PERCHLORATE; NUCLEIC ACID BASE; UNCLASSIFIED DRUG;

ARTICLE; DEPURINATION; DILUTION; ELUTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROLYSIS; PRECIPITATION; PRIORITY JOURNAL; REACTION ANALYSIS;

EID: 34748907854     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.06.002     Document Type: Article

References (12)

1. Srinivasan M., Sedmak D., and Jewell S. Effect of fixatives and tissue processing on the content and integrity of nucleic acids. Am. J. Pathol. 161 (2002) 1961-1971
2. Rait V.K., Zhang Q., Fabris D., Mason J.T., and O'Leary T.J. Conversions of formaldehyde-modified 2′-deoxyadenosine 5′-monophosphate in conditions modeling formalin-fixed tissue dehydration. J. Histochem. Cytochem. 54 (2006) 301-310
3. Masuda N., Ohnishi T., Kawamoto S., Monden M., and Obuko K. Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples. Nucleic Acids Res. 27 (1999) 4436-4443
4. Perrett D. HPLC of Small Molecules: A Practical Approach. In: Lim C.K. (Ed). Nucleotides, nucleosides, and bases (1986), IRL Press, Oxford, UK 221-259
5. Uesugi T., Sano K., Uesawa Y., Ikegama Y., and Mori K. Ion-pair reversed-phase high-performance liquid chromatography of adenine nucleotides and nucleosides using triethylamine as a counterion. J. Chromatogr. B 703 (1997) 63-74
6. Kawamoto Y., Shinozuka K., Kunitomo M., and Haginaka J. Determination of ATP and its metabolites released from rat caudal artery by isocratic ion-pair reversed-phase liquid chromatography. Anal. Biochem. 262 (1998) 33-38
7. Danilyuk N.K., Yastrebov S.I., Artamonova T.P., and Popov S.G. A simplified version of the Maxim-Gilbert method for the primary structure determination of oligonucleotides and DNA fragments. Bioorg. Khim. 12 (1986) 1185-1188
8. Mironova N.L., Pyshnyi D.V., Shtadler D.V., Fedorova A.A., Vlassov V.V., and Zenkova M.A. RNase T1 mimicking artificial ribonuclease. Nucleic Acids Res. 35 (2007) 2356-2367
9. Efimov V.A., Chakhmakhcheva O.G., Archdeacon J., Fernandez J.M., Fedorkin O.N., Dorokhov Y.L., and Atabekov J.G. Detection of the 5′-cap structure of messenger RNAs with the use of the cap-jumping approach. Nucleic Acids Res. 29 (2001) 4751-4759
10. T. Kuroita, H. Kamimura, B. Kawakami, Y. Kawamura, Method for isolating ribonucleic acid, U.S. patent 5990302 (1999).
11. 4 -diethyl ether catalyst. Turk. J. Chem. 26 (2002) 251-254
12. Frost C.G., and Hartley J.P. New applications of indium catalysts in organic synthesis. Mini-Rev. Org. Chem. 1 (2004) 1-7