The flawed scientific basis of the Altered Nuclear Transfer-Oocyte Assisted Reprogramming (ANT-OAR) proposal

Stem Cell Reviews

Volumn 3, Issue 1, 2007, Pages 60-65

  Byrnes, W Malcolm ^a  

^a HOWARD UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Altered nuclear transfer; Altered nuclear transfer oocyte assisted reprogramming; ANT; ANT OAR; Bioethics; Embryonic stem cells; Nanog

Indexed keywords

CDX2 PROTEIN, HUMAN; DNA BINDING PROTEIN; HOMEODOMAIN PROTEIN; NANOG PROTEIN, HUMAN; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; CELL NUCLEUS TRANSPLANTATION; CYTOLOGY; EMBRYO RESEARCH; ETHICS; EVALUATION; GENE EXPRESSION REGULATION; GENETICS; HUMAN; KNOWLEDGE BASE; METHODOLOGY; NUCLEAR REPROGRAMMING; OOCYTE; PHYSIOLOGY; PLURIPOTENT STEM CELL;

ANIMALS; DNA-BINDING PROTEINS; EMBRYO RESEARCH; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HOMEODOMAIN PROTEINS; HUMANS; KNOWLEDGE BASES; NUCLEAR REPROGRAMMING; NUCLEAR TRANSFER TECHNIQUES; OOCYTES; PLURIPOTENT STEM CELLS; RESEARCH DESIGN;

EID: 34748869672     PISSN: 15508943     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12015-007-0014-6     Document Type: Article

References (30)

1. Arkes, H., Austriaco, N., Berg, T., Brugger, E. C., Cameron, N. M., Capizzi, J., et al. (2005). Production of pluripotent stem cells by oocyte assisted reprogramming: Joint statement with signatories. National Catholic Bioethics Quarterly, 5, 579-583. Also available at: www.eppc.org/ publications/pubID.2374/pub_details.asp. Last accessed 1-02-07.
2. Hurlbut, W. (2004). Session 6: Seeking morally unproblematic sources of human stem cells. Transcript, President's Council on Bioethics, December 4, 2004. Available at: www.bioethics.gov/transcripts/dec04/session6.html. Last accessed 9-17-06.
3. Meissner, A., & Jaenisch, R. (2006). Generation of nuclear transfer-derived pluripotent ES cells from cloned Cdx2-deficient blastocysts. Nature, 439, 21221-21225.
4. Chawengsaksophak, K., de Graaf, W., Rossant, J., Deschamps, J., & Beck, F. (2004). Cdx2 is essential for axial elongation in mouse development. Proceedings of the National Academy of Sciences, 101, 7641-7645.
5. Strumpf, D., Mao, C.-A., Yamanaka, Y., Ralston, A., Chawengsaksophak, K., Beck, F., et al, (2005). Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Development, 32, 2093-2102.
6. Mitsui, K., Tokuzawa, Y., Itoh, H., Segawa, K., Murakami, M., Takahashi, K., et al. (2003). The homeoprotein Nanog is required for maintenance of pluripotency in mouse epiblast and ES cells. Cell, 113, 631-642.
7. Chambers, I., Colby, D., Robertson, M., Nichols, J., Lee, S., Tweedie, S., et al. (2003). Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells. Cell, 113, 643-656.
8. See articles on the Communio website at: www.communio-icr.com/ant.htm, especially: Walker, A. Reasonable doubts. A reply to E. Christian Brugger. Communio 2005; 32:770-783. Last accessed 1-02-07.
9. Austriaco, N. (2006). The moral case for ANT-derived pluripotent stem cell lines. National Catholic Bioethics Quarterly, 6, 517-537.
10. Sun, Y., Li, H., Yang, H., Rao, M., & Zhan, M. (2006). Mechanisms controlling embryonic stem cell, self-renewal and differentiation. Critical Reviews in Eukaryotic Gene Expression, 16, 211-231.
11. Noggle, S., James, D., & Brivanlou, A. (2005). A molecular basis for human embryonic stem cell pluripotency. Stem Cell Rev, 1, 111-118.
12. Boyer, L., Mathur, D., & Jaenisch, R. (2006). Molecular control of pluripotency. Current Opinion in Genetics & Development, 16, 455-462.
13. Gan, Q., Yoshida, T., McDonald, O., & Ownes, G. (2007). Concise Review: Epigenetic mechanisms contribute to pluripotency and cell lineage determination of embryonic stem cells. Stem Cells, 25, 2-9.
14. Loh, Y.-.H., Wu, Q., Chew, J.-L., Vega, V. B., Zhang, W., Chen, X., et al. (2006). The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells. Nature Genetics, 38, 431-440.
15. Boyer, L., Lee, T., Cole, M., Johnstone, S. E., Levine, S. S., Zucker, J. P., et al. (2005). Core transcriptional regulatory circuitry in human embryonic stem cells. Cell, 122, 947-956.
16. Rodda, D., Chew, J.-L., Lim, L.-H., Loh, Y H., Wang, B., Ng, H. H., et al. (2005). Transcriptional regulation of Nanog by OCT4 and SOX2. Journal of Biological Chemistry, 280, 24731-24737.
17. Pan, G., Li, J., Zhou, Y., Zheng, H., & Pei, D. (2006). A negative feedback, loop of transcription factors that controls stem cell pluripotency and self-renewal. FASEB Journal, 20, E1-E9.
18. Silva, J., Chambers, I., Pollard, S., & Smith, A. (2006). Nanog promotes transfer of pluripotency after cell fusion. Nature, 441, 997-1001.
19. Ivanova, N., Dobrin, R., Lu, R., Kotenko, I., Levorse, J., DeCoste, C., et al. (2006). Dissecting self-renewal in stem cells with RNA interference. Nature, 442, 533-538.
20. Takahashi, K., & Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 126, 1-14.
21. Check, E. (2007). Dolly: A hard, act to follow. Nature, 445, 802.
22. Hamatani, T., Carter, M., Sharov, A., & Ko, M. (2004). Dynamics of global, gene expression changes during mouse preimplantation development. Developmental Cell, 6, 117-131.
23. Deb, K., Sivaguru, M., Yong, H., & Roberts, R. (2006). Cdx2 gene expression and trophectoderm lineage specification in mouse embryos. Science, 311, 992-996.
24. Zernicka-Goetz, M. (2006). The first cell-fate decisions in the mouse embryo: Destiny is a matter of both chance and choice. Current Opinion in Genetics & Development, 16, 406-412.
25. Piotrowska, K., Wianny, F. Pedersen, R., & Zernicka-Goetz, M. (2001). Blastomeres arising from the first cleavage division have distinguishable fates in normal mouse development. Development, 19, 3739-3748.
26. Motosugi, N., Bauer, T., Polanski, Z., Solter, D., & Hiiragi, T. (2005). Polarity of the mouse embryo is established at blastocyst and is not prepatterned. Genes & Development, 19, 1081-1092.
27. Kennedy, D. (2006). Editorial expression of concern. Science, 314, 592.
28. Vogel, G. (2006). Fraud investigation clouds paper on early cell fate. Science, 314, 1367-1368.
29. Wei, C., Miura, T., Robson, P., Lim, S. K., Xu, X. Q., Lee, M. Y., et al. (2005). Transcriptome profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state. Stem Cells, 23, 166-185.
30. Solter, D. (2005). Politically correct human embryonic stem cells? New England Journal of Medicine, 353, 2321-2323.