Assays for in vitro monitoring of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cell migration

Nature Protocols

Volumn 1, Issue 6, 2007, Pages 2933-2939

  Goncharova, Elena A ^a   Goncharov, Dmitry A ^a   Krymskaya, Vera P ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL MOTION; CYTOLOGY; HUMAN; ISOTOPE LABELING; LUNG; METHODOLOGY; PHYSIOLOGY; PULMONARY ARTERY; SMOOTH MUSCLE FIBER;

CELL MOVEMENT; HUMANS; ISOTOPE LABELING; LUNG; MYOCYTES, SMOOTH MUSCLE; PULMONARY ARTERY;

EID: 34548845557     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.434     Document Type: Article

References (9)

1. Boyden, S. Chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes. J. Exp. Med. 115, 453-466 (1962).
2. Zigmond, S.H. Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. J.Cell Biol. 75, 606-616 (1977).
3. Goncharova, E.A. et al. Cyclic AMP-mobilizing agents and glucocorticoids modulate human smooth muscle cell migration. Am. J. Respir. Cell Mol. Biol. 29, 19-27 (2003).
4. Goncharova, E.A. et al. Activation of p38 MAP-kinase and caldesmon phosphorylation are essential for urokinase-induced human smooth muscle cell migration. Biol. Chem. 383, 115-126 (2002).
5. Goncharova, E.A. et al. PI3K is required for proliferation and migration of human pulmonary vascular smooth muscle cells. Am. J. Physiol. 283, L354-L363 (2002).
6. Goncharova, E., Goncharov, D., Noonan, D. & Krymskaya, V.P. TSC2 modulates actin cytoskeleton and focal adhesion through TSC1-binding domain and the Rac1 GTPase. J. Cell Biol. 167, 1171-1182 (2004).
7. Goncharova, E.A., Goncharov, D.A., Lim, P.N., Noonan, D. & Krymskaya, V.P. Modulation of cell migration and invasiveness by tumor suppressor TSC2 in lymphangioleiomyomatosis. Am. J. Respir. Cell Mol. Biol. 34, 473-480 (2006).
8. Krymskaya, V.P. Tumour suppressors hamartin and tuberin: Intracellular signalling. Cell. Signal. 15, 729-739 (2003).
9. Inoki, K., Corradetti, M.N. & Guan, K.-L. Dysregulation of the TSC-mTOR pathway in human disease. Nat. Genet. 37, 19-24 (2005).