Assessment of the use of high-performance capillary gel electrophoresis to differentiate isolates of Globodera spp.

Nematology

Volumn 8, Issue 1, 2006, Pages 139-146

  Da Cunha, Maria José M ^a,b   Conceição, Isabel Luci P M ^b   De Abrantes, Isabel Maria O ^b   De A Santos, M Susana N ^b  

^a INSTITUTO POLITÉCNICO DE COIMBRA   (Portugal)

^b UNIVERSITY OF COIMBRA   (Portugal)

Author keywords

Capillary gel electrophoresis; Globodera pallida; Globodera rostochiensis; Potato cyst nematodes; Protein analysis

Indexed keywords

ASSESSMENT METHOD; ELECTROKINESIS; NEMATODE; PATHOTYPE; PROTEIN; SOFTWARE;

EUCLIDIA; GLOBODERA; GLOBODERA PALLIDA; GLOBODERA ROSTOCHIENSIS; NEMATODA; SOLANUM TUBEROSUM;

EID: 34548843350     PISSN: 13885545     EISSN: 15685411     Source Type: Journal    
DOI: 10.1163/156854106776179980     Document Type: Article

References (33)

1. BAKKER, J. (1987). Protein variation in cyst nematodes. Ph.D. Thesis. Agricultural University of Wageningen, Wageningen, The Netherlands.
2. BENDEZU, I.F., EVANS, K., BURROWS, P.R., DE POMERAI, D. & CANTO SAENZ, M. (1998). Inter and intra-specific genomic variability of the potato cyst nematodes Globodera pallida and G. rostochiensis from Europe and South America using RAPD-PCR. Nematologica 44, 49-61.
3. BLOK, V.C., PHILLIPS, M.S. & HARROWER, B.E. (1997). Comparison of British populations of potato cyst nematodes with populations from continental Europe and South America using RAPDs. Genome 40, 286-293.
4. BURROWS, P.R., HALFORD, P.D. & EVANS, K. (1996). Estimation of genomic variation between British populations of the potato cyst nematode Globodera pallida using RAPD-PCR. Molecular Ecology 5, 697-701.
5. CANTO SAENZ, M. & DE SCURRAH, M.M. (1977). Races of the potato cyst nematode in the Andean region and a new system of classification. Nematologica 23, 340-349.
6. CONCEIÇÃO, I.L.P.M. DA, SANTOS, M.C.V. DOS, ABRANTES, I.M. DE O. & SANTOS, M.S.N. DE A. (2003). Using RAPD markers to analyse genetic diversity in Portuguese potato cyst nematodes populations. Nematology 5, 137-143.
7. CUNHA, M.J.M. DA (2001). Variabilidade genética de populações portuguesas de nemátodes-de- quisto da batateira, Globodera spp. Tese de Doutoramento. Universidade de Coimbra, Coimbra, Portugal, 256 pp.
8. CUNHA, M.J.M. DA, BOSSIS, M., ABRANTES, I.M. DE O., SANTOS, M.S.N. DE A. & MUGNIÉRY, D. (2000). Protein variability among Portuguese and other populations of Globodera rostochiensis revealed by two-dimensional gel electrophoresis with computed image analysis. Nematology 2, 461-471.
9. CUNHA, M.J.M. DA, CONCEIÇÃO, I.L.P.M. DA, ABRANTES, I.M. DE O., EVANS, K. & SANTOS, M.S.N. DE A. (2004). Characterisation of potato cyst nematode populations from Portugal. Nematology 6, 55-58.
10. EPPO (1985). Conclusions of the EPPO Workshop on Cyst Nematodes, Munster (FRG), 26-28 June, 1984. EPPO Bulletin 15, 121-122.
11. FENWICK, D.W. (1949). Methods for the recovery and counting of cysts of Heterodera schachtii from soil. Journal of Helminthology 18, 155-172.
12. a Reunião Bienal da Sociedade Portuguesa de Fitopatologia, Oeiras, pp. 190-195.
13. FLEMING, C.C. & MARKS, R.J. (1982). A method for quantitative estimation of Globodera rostochiensis and Globodera pallida in mixed species samples. Records of Agricultural Research of the Department of Agriculture of Northern Ireland 30, 67-70.
14. FLEMING, C.C. & MARKS, R.J. (1983). The identification of the potato cyst nematodes Globodera rostochiensis and G. pallida by isoelectric focusing of proteins on polyacrylamide gels. Annals of Applied Biology 103, 277-281.
15. FOLKERTSMA, R.T. (1997). Genetic diversity of the potato cyst nematode in The Netherlands. Ph.D. Thesis, Agricultural University of Wageningen, Wageningen, The Netherlands, 88 pp.
16. FOLKERTSMA, R.T., ROUPPE VAN DER VOORT, J.N.A.M., VAN GENT-PELZER, M.P.E., DE GROOT, K.E., VAN DER BOS, W.J., SCHOTS, A., BAKKER, J. & GOMMERS, F.J. (1994). Inter- and intraspecific variation between populations of Globodera rostochiensis and G. pallida revealed by Random Amplified Polymorphic DNA. Phytopathology 84, 807-811.
17. FOLKERTSMA, R.T., ROUPPE VAN DER VOORT, J.N.A.M., DE GROOT, K.E., VAN ZANDVOORT, P.M., SCHOTS, A., GOMMERS, F.J., HELDER, J. & BAKKER, J. (1996). Gene pool similarities of potato cyst nematode populations assessed by AFLP analysis. Molecular Plant-Microbe Interactions 9, 47-54.
18. FOX, P.C. & ATKINSON, H.J. (1984a). Glucose phosphate isomerase polymorphism in field populations of the potato cyst nematodes, Globodera rostochiensis and G. pallida. Annals of Applied Biology 104, 503-506.
19. FOX, P.C. & ATKINSON, H.J. (1984b). Isoelectric focusing of general protein and specific enzymes from pathotypes of Globodera rostochiensis and G. pallida, Parasitology 88, 131-139.
20. FULLAONDO, A., SALAZAR, A., BARRENA, E. & RITTER, E. (1997). Comparison of molecular patterns and virulence behaviour of potato cyst nematodes. Fundamental and Applied Nematology 20, 425-433.
21. GONZALEZ, J.A., PHILLIPS, M.S.& TRUDGILL, D.L. (1995). RFLP analysis in Canary Islands and North European populations of potato cyst nematodes (Globodera spp.) I. Analysis of high copy fragments. Nematologica 41, 468-479.
22. HINCH, J.M., ALBERDI, F., SMITH, S.C., WOODWARD, J.R. & EVANS, K. (1998). Discrimination of European and Australian Globodera rostochiensis and G. pallida pathotypes by high-performance capillary electrophoresis. Fundamental and Applied Nematology 21, 123-128.
23. KORT, J., ROSS, H., RUMPENHORST, H.J. & STONE, A.R. (1977). An international scheme for identifying and classifying pathotypes of potato cyst nematodes Globodera rostochiensis and G. pallida. Nematologica 23, 333-339.
24. MUGNIÉRY, D., PHILLIPS, M.S., RUMPENHORST, H.J., STONE, A.R., TREUR, A. & TRUDGILL, D.L. (1989). Assessment of partial resistance of potato to, and pathotype and virulence differences in, potato cyst nematodes. EPPO Bulletin 19, 7-25.
25. NIJBOER, H. & PARLEVLIET, J.E. (1990). Pathotype-specificity in potato cyst nematodes, a reconsideration. Euphytica 49, 39-47.
26. PHILIPS, M.S. & TRUDGILL, D.L. (1985). Pot and field assessment of partial resistance of potato clones to different populations and densities of Globodera pallida. Nematologica 31, 433-442.
27. PHILLIPS, M.S. & TRUDGILL, D.L. (1998). Variation of virulence, in terms of quantitative reproduction of Globodera pallida populations, from Europe and South America, in relation to resistance from Solanum vernei and S. tuberosum ssp. andigena CPC 2802. Nematologica 44, 409-423.
28. PHILLIPS, M.S., HARROWER, B.E., TRUDGILL, D.L., CATLEY, M.A. & WAUGH, R. (1992). Genetic variation in British populations of Globodera pallida as revealed by isozyme and DNA analyses. Nematologica 38, 304-319.
29. ROHLF, F.J. (1993). NTSYSpc. Numerical taxonomy and multivariate analysis system. Version 1.80. Setauket, LI, NY, USA, Applied Biostatics, Inc.
30. ROHLF, F.J. (1998). NTSYSpc. Numerical Taxonomy and Multivariate Analysis System. Version 2.0. User Guide. Setauket, LI, NY, USA, Applied Biostatics, Inc.
31. SANTOS, M.S.N. DE A., EVANS, K., ABREU, C.A., MARTINS, F.F. & ABRANTES, I.M. DE O. (1995). A review of potato cyst nematodes in Portugal. Nematologia Mediterranea 23, 35-42.
32. SOKAL, R.R. & ROHLF, F.J. (1962). The comparison of dendrograms by objective methods. Taxonomie 11, 33-40.
33. TRUDGILL, D.L. (1985). Potato cyst nematodes: a critical review of the current pathotyping scheme. EPPO Bulletin 15, 273-279.