3′ End cDNA amplification using classic RACE

Nature Protocols

Volumn 1, Issue 6, 2007, Pages 2742-2745

  Scotto Lavino, Elizabeth ^a   Du, Guangwei ^a   Frohman, Michael A ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; POLYADENYLIC ACID;

ARTICLE; CHEMISTRY; DNA FLANKING REGION; METHODOLOGY; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; SYNTHESIS;

3' FLANKING REGION; DNA, COMPLEMENTARY; POLY A; POLYMERASE CHAIN REACTION; RESEARCH DESIGN; REVERSE TRANSCRIPTION;

EID: 34548828779     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2006.481     Document Type: Article

References (6)

1. Frohman, M.A., Dush, M.K. & Martin, G.R. Rapid production of full-length cDNAs from rare transcripts by amplification using a single gene-specific oligonucleotide primer. Proc. Natl Acad. Sci. USA 85, 8998-9002 (1988).
2. Scotto-Lavino, E., Du, G. & Frohman, M.A. 5′ End cDNA amplification using classic RACE. Nat. Protocols (2006). DOI: 10.1038/nprot.2006.480
3. Scotto-Lavino, E., Du, G. & Frohman, M.A. 5′ End cDNA amplification using new RACE. Nat. Protocols (2007). DOI: 10.1038/nprot.2006.479
4. Wickens, M. & Stephenson, P. Role of the conserved AAUAAA sequence: Four AAUAAA point mutants prevent mRNA 3′ end formation. Science 226, 1045-1050 (1984).
5. Coleclough, C. Use of primer-restriction end adapters in cDNA cloning. Methods Enzymol. 154, 64-83 (1987).
6. Zhang, Y. In Generation of cDNA Libraries (ed. Ying, S.-Y.) 13-24 (Humana Press, Totowa, NJ, 2003).