Expression of human BK ion channels in Sf9 cells, their purification using metal affinity chromatography, and functional reconstitution into planar lipid bilayers

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 857, Issue 2, 2007, Pages 315-321

  Dalziel, Julie E ^a   Wong, Shu Shyan ^a   Phung, Thai ^a   Zhang, Yan Li ^a   Dunlop, James ^a  

^a AGRESEARCH GRASSLANDS   (New Zealand)

Author keywords

His tag; IMAC; Ion channel; Planar lipid bilayer; Purification; Sf9 insect cell

Indexed keywords

AFFINITY CHROMATOGRAPHY; CELLS; LIPIDS; MAMMALS; POTASSIUM; PURIFICATION;

ION CHANNELS; PLANAR LIPID BILAYERS; SF9 INSECT CELLS;

GENE EXPRESSION;

CALCIUM ACTIVATED POTASSIUM CHANNEL; HISTIDINE; PAXILLINE;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BACULOVIRUS; CELL MEMBRANE CONDUCTANCE; CHROMATOGRAPHY; ELECTROPHYSIOLOGY; IMMOBILISED METAL AFFINITY CHROMATOGRAPHY; INSECT CELL; LIPID BILAYER; NONHUMAN; NUCLEOTIDE SEQUENCE; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEOLIPOSOME; PURIFICATION;

ANIMALS; CHROMATOGRAPHY, AFFINITY; DROSOPHILA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HISTIDINE; HUMANS; LIPID BILAYERS; METALS; OLIGOPEPTIDES; POTASSIUM CHANNELS, CALCIUM-ACTIVATED;

HEXAPODA;

EID: 34548825401     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2007.07.033     Document Type: Article

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