Validation of the MycoAlign system for Mycobacterium spp. identification

Diagnostic Microbiology and Infectious Disease

Volumn 59, Issue 1, 2007, Pages 105-108

  Olsen, Randall J ^a,b   Cernoch, Patricia A ^b   Austin, Celest M ^a   Graviss, Edward A ^a   Farkas, Daniel H ^b,c   Land, Geoffrey A ^b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b HOUSTON METHODIST HOSPITAL   (United States)

^c GeneNews Limited   (Canada)

Author keywords

16S; hsp65; Internal transcribed region; ITS 1; Molecular diagnostics; MycoAlign; Mycobacteria; PCR; Sequencing

Indexed keywords

BACTERIAL DNA; INTERNAL TRANSCRIBED SPACER;

ACID FAST BACTERIUM; ARTICLE; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATION; HUMAN; LABORATORY DIAGNOSIS; MYCOBACTERIUM; NONHUMAN; PRIORITY JOURNAL; PROBABILITY; SCORING SYSTEM; SPECIES IDENTIFICATION; VALIDATION PROCESS;

BACTERIAL PROTEINS; CHAPERONINS; GENOTYPE; HUMANS; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM; REAGENT KITS, DIAGNOSTIC; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S;

EID: 34548634191     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2007.04.012     Document Type: Article

References (12)

1. Clarridge III J.E. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases. Clin. Microbiol. Rev. 17 (2004) 840-862 [table of contents]
2. Cornfield D.B., Beavis K.G., Greene J.A., Bojak M., and Bondi J. Mycobacterial growth and bacterial contamination in the mycobacteria growth indicator tube and BACTEC 460 culture systems. J. Clin. Microbiol. 35 (1997) 2068-2071
3. Dobner P., Feldmann K., Rifai M., Loscher T., and Rinder H. Rapid identification of mycobacterial species by PCR amplification of hypervariable 16S rRNA gene promoter region. J. Clin. Microbiol. 34 (1996) 866-869
4. Kirschner P., Springer B., Vogel U., Meier A., Wrede A., Kiekenbeck M., Bange F.C., and Bottger E.C. Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J. Clin. Microbiol. 31 (1993) 2882-2889
5. Mohamed A.M., Kuyper D.J., Iwen P.C., Ali H.H., Bastola D.R., and Hinrichs S.H. Computational approach involving use of the internal transcribed spacer 1 region for identification of Mycobacterium species. J. Clin. Microbiol. 43 (2005) 3811-3817
6. Palomino J.C. Conventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field. Eur. Respir. J. 26 (2005) 339-350
7. Patel J.B., Leonard D.G., Pan X., Musser J.M., Berman R.E., and Nachamkin I. Sequence-based identification of Mycobacterium species using the MicroSeq 500 16S rDNA bacterial identification system. J. Clin. Microbiol. 38 (2000) 246-251
8. Pfyffer G., Brown-Elliot B., and Wallace R. Mycobacterium: general characteristics, isolation, and staining procedures. In: Murray P., Baron E., Jorgensen J., Pfaller M., and Yolken R. (Eds). Manual of Clinical Microbiology (2003), ASM Press, Washington, DC 532-559
9. Roth A., Fischer M., Hamid M.E., Michalke S., Ludwig W., and Mauch H. Differentiation of phylogenetically related slowly growing mycobacteria based on 16S-23S rRNA gene internal transcribed spacer sequences. J. Clin. Microbiol. 36 (1998) 139-147
10. Roth A., Reischl U., Streubel A., Naumann L., Kroppenstedt R.M., Habicht M., Fischer M., and Mauch H. Novel diagnostic algorithm for identification of mycobacteria using genus-specific amplification of the 16S-23S rRNA gene spacer and restriction endonucleases. J. Clin. Microbiol. 38 (2000) 1094-1104
11. Soini H., and Musser J.M. Molecular diagnosis of mycobacteria. Clin. Chem. 47 (2001) 809-814
12. Vincent V., Brown-Elliott B., Jost K., and Wallace R. Mycobacterium: phenotypic and genotypic identification. In: Murray P., Baron E., Jorgensen J., Pfaller M., and Yolken R. (Eds). Manual of Clinical Microbiology (2003), ASM Press, Washington, DC 560-584