Cloning and characterization of a haloarchaeal heat shock protein 70 functionally expressed in Escherichia coli

FEMS Microbiology Letters

Volumn 275, Issue 1, 2007, Pages 168-174

  Zhang, Hao ^a   Lin, Lu ^a   Zeng, Chi ^a   Shen, Ping ^a   Huang, Yu Ping ^a  

^a WUHAN UNIVERSITY   (China)

Author keywords

ATPase activity; Haloarchaea; hsp70; Hsp70

Indexed keywords

ADENOSINE TRIPHOSPHATASE; HEAT SHOCK PROTEIN 40; HEAT SHOCK PROTEIN 70; POTASSIUM CHLORIDE; SODIUM CHLORIDE;

ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENE IDENTIFICATION; GENE ISOLATION; GENE LOCUS; GENE SEQUENCE; GENETIC TRANSCRIPTION; HALOARCULA; HALOPHILE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS;

ADAPTATION, PHYSIOLOGICAL; ADENOSINE TRIPHOSPHATASES; ARCHAEAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENES, ARCHAEAL; GENETIC COMPLEMENTATION TEST; HALOBACTERIACEAE; HEAT; HSP70 HEAT-SHOCK PROTEINS; MOLECULAR SEQUENCE DATA; OSMOSIS; RECOMBINANT PROTEINS; TRANSCRIPTION INITIATION SITE;

ESCHERICHIA COLI; HALOBACTERIA; HALOPHILIC ARCHAEON; NATRINEMA;

EID: 34548563855     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2007.00881.x     Document Type: Article

References (28)

1. Alfano C McMacken R (1989) Heat shock protein-mediated disassembly of nucleoprotein structures is required for the initiation of bacteriophage lambda DNA replication. J Biol Chem 264 : 10709 10718.
2. Bradford MM (1976) A rapid and sensitive method for quantition of proteins utilizing the principles of protein-dye binding. Anal Biochem 72 : 248 254.
3. Buchberger A, Valencia A, McMacken R, Sander C Bukau B (1994) The chaperone function of DnaK requires the coupling of ATPase activity with substrate binding through residue E171. EMBO J 13 : 1687 1695.
4. Bukau B Horwich AL (1998) The Hsp70 and Hsp60 chaperone machines. Cell 92 : 351 366.
5. Connaris H, Chaudhuri JB, Danson MJ Hough DW (1999) Expression, reactivation, and purification of enzymes from Haloferax volcanii in Escherichia coli. Biotechnol Bioeng 64 : 38 45.
6. Ellis RJ van der Vies SM (1991) Molecular chaperones. Annu Rev Biochem 60 : 321 347.
7. Hickey AJ, Conway de Macario E Macario AJ (2002) Transcription in the archaea: basal factors, regulation, and stress-gene expression. Crit Rev Biochem Mol Biol 37 : 537 599.
8. James P, Pfund C Craig EA (1997) Functional specificity among Hsp70 molecular chaperones. Science 275 : 387 389.
9. Lanzetta PA, Alvarez LJ, Reinach PS Candia OA (1979) An improved assay for nanomole amounts of inorganic phosphate. Anal Biochem 100 : 95 97.
10. Liberek K, Marszalek J, Ang D, Georgopoulos C Zylicz M (1991) Escherichia coli DnaJ and GrpE heat shock proteins jointly stimulate ATPase activity of DnaK. Proc Natl Acad Sci USA 88 : 2874 2878.
11. Macario AJ, Malz M Conway de Macario E (2004) Evolution of assisted protein folding: the distribution of the main chaperoning systems within the phylogenetic domain archaea. Front Biosci 9 : 1318 1332.
12. Macario AJ, Brocchieri L, Shenoy AR Conway de Macario E (2006) Evolution of a protein-folding machine: genomic and evolutionary analyses reveal three lineages of the archaeal hsp70(dnaK) gene. J Mol Evol 63 : 74 86.
13. Madern D, Ebel C Zaccai G (2000) Halophilic adaptation of enzymes. Extremophiles 4 : 91 98.
14. Mayer MP Bukau B (2005) Hsp70 chaperones: cellular functions and molecular mechanism. Cell Mol Life Sci 62 : 670 684.
15. Mogk A, Bukau B, Lutz R Schumann W (1999) Construction and analysis of hybrid Escherichia coli-Bacillus subtilis dnaK genes. J Bacteriol 181 : 1971 1974.
16. Paek KH Walker GC (1987) Escherichia coli dnaK-null mutants are inviable at high temperature. J Bacteriol 169 : 283 290.
17. QIAGEN (2002) A Handbook for High-Level Expression and Purification of 6xHis-Tagged Proteins. QIAGEN GmbH, Hilden.
18. Reeve JN (2003) Archaeal chromatin and transcription. Mol Microbiol 48 : 587 598.
19. Sambrook J Russell DW (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor Laboratory Press, New York.
20. Shi W, Tang XF, Huang Y, Gan F, Tang B Shen P (2006) An extracellular halophilic protease SptA from a halophilic archaeon Natrinema sp. J7: gene cloning, expression and characterization. Extremophiles 10 : 599 606.
21. Suppini JP, Amor M, Alix JH Ladjimi MM (2004) Complementation of an Escherichia coli DnaK defect by Hsc70-DnaK chimeric proteins. J Bacteriol 186 : 6248 6253.
22. Tokunaga H, Hara S, Arakawa T, Ishibashi M, Gupta RS Tokunaga M (1999) Identification and partial purification of DnaK homologue from extremely halophilic archaebacteria, Halobacterium cutirubrum. J Protein Chem 18 : 837 844.
23. Torarinsson E, Klenk HP Garrett RA (2005) Divergent transcriptional and translational signals in Archaea. Environ Microbiol 7 : 47 54.
24. Wang JY, Sarker AH, Cooper PK Volkert MR (2004) The single-strand DNA binding activity of human PC4 prevents mutagenesis and killing by oxidative DNA damage. Mol Cell Biol 24 : 6084 6093.
25. Wickner S, Skowyra D, Hoskins J McKenney K (1992) DnaJ, DnaK, and GrpE heat shock proteins are required in oriP1 DNA replication solely at the RepA monomerization step. Proc Natl Acad Sci USA 89 : 10345 10349.
26. Ye X, Ou J, Ni L, Shi W Shen P (2003) Characterization of a novel plasmid from extremely halophilic Archaea: nucleotide sequence and function analysis. FEMS Microbiol Lett 221 : 53 57.
27. Yoshimune K, Galkin A, Kulakova L, Yoshimura T Esaki N (2005) Cold-active DnaK of an Antarctic psychrotroph Shewanella sp. Ac10 supporting the growth of dnaK-null mutant of Escherichia coli at cold temperatures. Extremophiles 9 : 145 150.
28. Zmijewski MA, Macario AJ Lipinska B (2004) Functional similarities and differences of an archaeal Hsp70(DnaK) stress protein compared with its homologue from the bacterium Escherichia coli. J Mol Biol 336 : 539 549.