Rapid molecular identification of the harmful freshwater dinoflagellate Peridinium in various life stages using genus-specific single-cell PCR

Journal of Applied Phycology

Volumn 19, Issue 5, 2007, Pages 467-470

  Ki, Jang Seu ^a,b   Han, Myung Soo ^a  

^a Hanyang University   (South Korea)

^b HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Hong Kong)

Author keywords

Dinoflagellate cyst; Planozygote; rDNA

Indexed keywords

DINOPHYCEAE; PERIDINIUM;

EID: 34548440744     PISSN: 09218971     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10811-007-9157-8     Document Type: Article

References (7)

1. Bolch CJS (2001) PCR protocols for genetic identification of dinoflagellates directly from single cysts and plankton. Phycologia 40:162-167
2. Coyne KJ, Cary SC (2005) Molecular approaches to the investigation of viable dinoflagellate cysts in natural sediments from estuarine environments. J Eukaryot Microbiol 52:90-94
3. Kawabata Z, Hirano Y (1995) Growth pattern and cellular nitrogen and phosphorus contents of the dinoflagellate Peridinium penardii (Lemm.) Lemm. causing a freshwater red tide in a reservoir. Hydrobiologia 312:115-120
4. Ki J-S, Han M-S (2005) Sequence-based diagnostics and phylogenetic approach of uncultured freshwater dinoflagellate Peridinium species based on single-cell sequencing of rDNA. J Appl Phycol 17:147-153
5. Ki J-S, Jang GY, Han M-S (2004) Integrated method for single-cell DNA extraction, PCR amplification, and sequencing of ribosomal DNA from harmful dinoflagellates Cochlodinium polykrikoides and Alexandrium catenella. Mar Biotechnol 6:587-593
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7. Rengefors K, Legrand C (2001) Toxicity in Peridinium aciculiferum-an adaptive strategy to outcompete other winter phytoplankton? Limnol Oceanogr 46:1990-1997