Rotifer digestive enzymes: Direct detection using the ELF technique

Hydrobiologia

Volumn 593, Issue 1, 2007, Pages 159-165

  Štrojsová, Martina ^a   Vrba, Jaroslav ^b  

^a UNIVERSITY OF SOUTH BOHEMIA   (Czech Republic)

^b INSTITUTE OF HYDROBIOLOGY   (Czech Republic)

Author keywords

N acetylhexosaminidase; Enzyme localization; Lipase; Phosphatase; Rotifers

Indexed keywords

BACTERIA; BATCH CELL CULTURE; DIGESTIVE SYSTEM; FLUORESCENCE; LIPASES;

ENZYME LABELLED FLUORESCENCE (ELF); ENZYME LOCALIZATION; HYDROLYTIC ENZYMES; PHOSPHATASES; ROTIFER DIGESTIVE ENZYMES;

ENZYME ACTIVITY;

DIGESTION; ENZYME ACTIVITY; FLATWORM; HYDROLYSIS;

BRACHIONUS ANGULARIS; KERATELLA COCHLEARIS; LECANE CLOSTEROCERCA; LORICA; ROTIFERA;

EID: 34548437029     PISSN: 00188158     EISSN: 15735117     Source Type: Journal    
DOI: 10.1007/s10750-007-9053-x     Document Type: Conference Paper

References (25)

1. Arndt, H., 1993. Rotifers as predators on components of the microbial web (bacteria, heterotrophic flagellates, ciliates)-a review. Hydrobiologia 255/256: 231-246.
2. Dyhrman, S. T. & B. Palenik, 1999. Phosphate stress in cultures and field populations of the dinoflagellate Prorocentrum minimum detected by a single-cell alkaline phosphatase assay. Applied Environmental Microbiology 65: 3205-3212.
3. Gonzáles-Gil, S., B. Keafer, R. V. M. Jovine, A. Aquilera, S. Lu & D. M. Anderson, 1998. Detection and quantification of alkaline phosphatase in single cells of phosphorus-starved marine phytoplankton. Marine Ecology Progress Series 164: 21-35.
4. Hara, K., H. Pangkey, K. Osatomi, K. Yatsuda, A. Hagiwara, K. Tachibana & T. Ishihara, 1997. Some properties of β-1,3-glucan hydrolyzing enzymes from the rotifer Brachionus plicatilis. Hydrobiologia 358: 89-94.
5. Herold, R. C. & N. D. Meadow, 1970. Age-related changes in ultrastructure and histochemistry of rotiferan organs. Journal of Ultrastructure 33: 203-218.
6. Herzig, A., 1987. The analysis of planktonic rotifer populations: a plea for long-term investigations. Hydrobiologia 147: 163-180.
7. Kleinow, W., H. Wratil, K. Kühle & B. Esch, 1991. Electron microscope studies of the digestive tract of Brachionus plicatilis (Rotifera). Zoomorphology 111: 67-80.
8. Kleinow, W., 1993. Chemical studies on Brachionus plicatilis: hydrolytic enzymes, integument proteins and composition of trophi. Hydrobiologia 255/256: 1-12.
9. Kleinow, W. & A. Röhrig, 1995. Enzyme activity measurements on isolated organs of Brachionus plicatilis (Rotifera). Hydrobiologia 313/314: 171-174.
10. Kühle, K. & W. Kleinow, 1985. Measurements of hydrolytic enzymes in homogenates from Brachionus plicatilis (Rotifera). Comparative Biochemistry and Physiology 81: 437-442.
11. Kühle, K. & W. Kleinow, 1989. Localization of hydrolytic enzymes within cellular fractions from Brachionus plicatilis (Rotifera). Comparative Biochemistry and Physiology 93: 565-574.
12. Kühle, K. & W. Kleinow, 1990. Glycosidases in Brachionus plicatilis (Rotifera). Comparative Biochemistry and Physiology 95: 393-402.
13. Lindemann, N. & W. Kleinow, 2000. A study of rotifer feeding and digestive processes using erythrocytes as microparticulate markers. Hydrobiologia 435: 27-41.
14. Nedoma, J., A. Štrojsová, J. Vrba, J. Komá rková & K. Šimek, 2003. Extracellular phosphatase activity of natural plankton studied with ELF97 phosphate: fluorescence quantification and labelling kinetics. Environmental Microbiology 5: 462-472.
15. Porter, K. G. & Y. S. Feig, 1980. The use of DAPI for identifying and counting aquatic microflora. Limnology and Oceanography 25: 943-948.
16. Rengefors, K., K. C. Ruttenberg, C. L. Haupert, C. D. Taylor, B. L. Howes & D. M. Anderson, 2003. Experimental investigation of taxon-specific response of alkaline phosphatase activity in natural freshwater phytoplankton. Limnology and Oceanography 48: 1167-1175.
17. Salt, G. W., 1987. The components of feeding behavior in rotifers. Hydrobiologia 147: 271-281.
18. Starkweather, P. L., 1995. Near-coronal fluid flow patterns and food cell manipulation in Brachionus calyciflorous. Hydrobiologia 313/314: 193-198.
19. Štrojsová, A., J. Vrba, J. Nedoma, J. Komá rková & P. Znachor, 2003. Seasonal study of extracellular phosphatase expression in the phytoplankton of a eutrophic reservoir. European Journal of Phycology 38: 295-306.
20. Štrojsová, M. & J. Vrba, 2005. Direct detection of digestive enzymes in planktonic rotifers using enzyme labelled fluorescence (ELF). Marine and Freshwater Research 56: 189-195.
21. Walz, N., 1997. Rotifer life history strategies and evolution in freshwater plankton communities. In: Streit, B., T. Städler & C. M. Lively (eds), Evolutionary Ecology of Freshwater Animals, Birkhäuser Verlag, Basel, 119-149.
22. Wurdak, E. S., 1987. Ultrastructure and histochemistry of the stomach of Asplanchna sieboldi. Hydrobiologia 147: 361-371.
23. Yoshida, T., 2005. Toward the understanding of complex population dynamics: planktonic community as a model system. Ecological Research 20: 511-518.
24. Yu, J.-P. & S.-J. Cui, 1997. Ultrastructure of the rotifer Brachionus plicatilis. Hydrobiologia 358: 95-103.
25. Zehnder, A. A. & P. R. Gorham, 1960. Factor influencing the growth of Microcystis aeruginosa Kütz. emend. Elenk. Canadian Journal of Microbiology 6: 645-660.