Purification and characterization of the antitumor protein from Chinese tartary buckwheat (Fagopyrum tataricum Gaertn.) water-soluble extracts

Journal of Agricultural and Food Chemistry

Volumn 55, Issue 17, 2007, Pages 6958-6961

  Guo, Xiaona ^a   Zhu, Kexue ^a   Zhang, Hui ^a   Yao, Huiyuan ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Antitumor activity; Structure analysis; Tartary buckwheat; Water soluble extracts

Indexed keywords

AMINO ACID; ANTINEOPLASTIC AGENT; PLANT EXTRACT; VEGETABLE PROTEIN; WATER;

ARTICLE; CELL DIVISION; CHEMISTRY; DRUG EFFECT; FAGOPYRUM; HUMAN; ISOLATION AND PURIFICATION; SOLUBILITY; TUMOR CELL LINE;

AMINO ACIDS; ANTINEOPLASTIC AGENTS, PHYTOGENIC; CELL DIVISION; CELL LINE, TUMOR; FAGOPYRUM; HUMANS; PLANT EXTRACTS; PLANT PROTEINS; SOLUBILITY; WATER;

FAGOPYRUM; FAGOPYRUM TATARICUM;

EID: 34548361954     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf071032+     Document Type: Article

References (25)

1. Tahir, I.; Farooq, S. Grain composition in some buckwheat cultivars (Fagopyrum spp.) with particular reference to protein fractions. Qual. Plant. Plant Foods Hum. Nutr. 1985, 35, 153-158.
2. Bonafaccia, G.; Gambelli, L.; Fabjan, N.; Kreft, I. Trace elements in flour and bran from common and tartary buckwheat. Food Chem. 2003, 83, 1-5.
3. Kawakmi, A.; Kayahara, H.; Ujihara, A. Properties and elimination of bitter components derived from tartary buckwheat (Fagopyrum tataricum) flour. Nippon Shokuhin Kagaku Kogaku Kaishi 1995, 42, 892-898.
4. Bonafaccia, G.; Marocchini, M.; Kreft, I. Composition and technological properties of the flour and bran from common and tartary buckwheat. Food Chem. 2003, 80, 9-15.
5. Fabjan, N.; Rode, J.; Kosir, I. J.; Wang, Z. H.; Zhang, Z.; Kreft, I. Tartary buckwheat (Fagopyrum tataricum Gaertn.) as a source of dietary rutin and quercitrin. J. Agric. Food Chem. 2003, 51, 6452-6455.
6. Li, S. Q.; Zhang, H. Q. Advances in the development of functional foods from buckwheat. Crit. Rev. Food Sci. Nutr. 2001, 41, 451-464.
7. Pomeranz, Y.; Robbins, G. S. Amino acids composition of buckwheat protein. J. Agric. Food Chem. 1972, 20, 270-274.
8. Ikeda, K.; Kishida, M. Digestibility of proteins in buckwheat seed. Fagopyrum 1993, 13, 21-24.
9. Guo, X. N.; Yao, H. Y. Fractionation and characterization of tartary buckwheat flour proteins. Food Chem. 2006, 98, 90-94.
10. Guo, X. N.; Yao, H. Y.; Chen, Z. X. In vitro digestibility of Chinese tartary buckwheat protein fractions: the microstructure and molecular weight distribution of their hydrolysates. J. Food Biochem. 2006, 30, 508-520.
11. Kayashita, J.; Shimaoka, I.; Nakajoh, M.; Yamazaki, M. Hypocholesterolemic effect of buckwheat protein extract in rats fed cholesterol enriched diets. Nutr. Res. 1995, 15, 691-698.
12. Kayashita, J.; Shimaoka, I.; Nakajoh, M.; Yamazaki, M.; Kato, N. Consumption of buckwheat protein lowers plasma cholesterol and raises fecal neutral sterols in cholesterol-fed rats because of its low digestibility. J. Nutr. 1997, 127, 1395-1400.
13. Tomotake, H.; Shimaoka, I.; Kayashita, J.; Yokoyama, F.; Nakajoh, M.; Kato, N. Stronger suppression of plasma cholesterol and enhancement of the fecal excretion of steroids by a buckwheat protein product than by a soy protein isolate in rats fed on a cholesterol-free diet. Biosci. Biotechnol. Biochem. 2001, 65, 1412-1414.
14. Tomotake, H.; Yamamoto, N.; Yanaka, N.; Ohinata, H.; Yamazaki, R.; Kayashita, J.; Kato, N. High protein buckwheat flour suppresses hypercholesterolemia in rats and gallstone formation in mice by hypercholesterolemic diet and body fat in rats because of its low protein digestibility. Nutrition (N. Y.) 2006, 22, 166-173.
15. Kayashita, J.; Shimaoka, I.; Yamazaki, M.; Kato, N. Buckwheat protein extract ameliorates atropine-induced constipation in rats. Curr. Adv. Buckwheat Res. 1995, 2, 941-946.
16. Kayashita, J.; Shimaoka, I.; Nakajoh, M.; Kato, N. Feeding of buckwheat protein extract reduces hepatic triglyceride concentration, adipose tissue weight, and hepatic lipogenesis in rats. J. Nutr. Biochem. 1996, 7, 555-559.
17. Liu, Z.; Ishikawa, W.; Huang, X.; Tomotake, H.; Kayashita, J.; Watanabe, H.; Nakajoh, M.; Kato, N. A buckwheat protein product suppresses 1,2-dimethyhydrazine-induced colon carcinogenesis in rats by reducing cell proliferation. J. Nutr. 2001, 131, 1850-1853.
18. Kayashita, J.; Shimaoka, I.; Nakajoh, M.; Kishida, N.; Kato, N. Consumption of a buckwheat protein extract retards 7,12-dimetylbenz(α) anthracene-induced mammary carcinogenesis in rats. Biosci. Biotechnol. Biochem. 1999, 63, 1837-1839.
19. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
20. Yang, J. T.; Wu, C. C.; Martinez, H. M. Calculation of protein conformation from circular dichroism. Methods Enzymol. 1986, 130, 208-269.
21. Chen, Y. H.; Yang, J. T.; Martinez, H. M. Determination of the secondary structures of proteins by circular dichroism and optical rotary dispersion. Biochem. 1972, 11, 4120-4131.
22. Carmichael, J.; DeGraff, W.; Gazdar, A.; Minna, J.; Mitchell, J. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 1987, 47, 936-942.
23. Ma, Q.; Hoper, M.; Anderson, N.; Rowlands, B. J. Effect of supplemental L-arginine in a chemical induced model of colorectal cancer. World J. Surg. 1996, 20, 1087-1091.
24. Ma, Q.; Williamson, K. E.; O'Rourke, D.; Rowlands, B. J. The effects of L-arginine on crypt cell hyperproliferation in colorectal cancer. J. Surg. Res. 1999, 81, 181-188.
25. Burns, R. A.; Milner, J. A. Effect of arginine on the carcinogenicity of 7,12-dimetylbenz (a) anthracene and N-methy-N-nitrosourea. Carcinogenesis 1984, 5, 1539-1542.