Determination of the minimum population size of pumas (Puma concolor) through fecal DNA analysis in two protected cerrado areas in the Brazilian Southeast

Biotropica

Volumn 39, Issue 5, 2007, Pages 647-654

  Miotto, Renata Alonso ^a   Rodrigues, Fernando Pacheco ^a   Ciocheti, Giordano ^b   Galetti Jr , Pedro Manoel ^a  

^a FEDERAL UNIVERSITY OF SÃO CARLOS   (Brazil)

^b UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Conservation; Cytochrome b; Individual identification; Metapopulation; Microsatellites; Noninvasive analysis; Species diagnosis

Indexed keywords

CONSERVATION MANAGEMENT; DNA; ENDANGERED SPECIES; FECES; FELID; GENETIC ANALYSIS; HABITAT LOSS; POPULATION SIZE; PROTECTED AREA; SPECIES CONSERVATION;

BRAZIL; SAO PAULO [BRAZIL]; SOUTH AMERICA;

LEOPARDUS PARDALIS; PUMA CONCOLOR;

EID: 34548229406     PISSN: 00063606     EISSN: 17447429     Source Type: Journal    
DOI: 10.1111/j.1744-7429.2007.00315.x     Document Type: Article

References (46)

1. Albaugh, G. P., V. Iyengar, and A. Lohani. 1992. Isolation of exfoliated colonic epithelial cells, a novel, non-invasive approach to the study of cellular markers. Int. J. Cancer. 52 : 347 350.
2. Anderson, C. R., Jr., F. G. Lindzey, and D. B. McDonald. 2004. Genetic structure of cougar populations across the Wyoming Basin: Metapopulation or megapopulation. J. Mammal. 85 : 1207 1214.
3. Avise, J. C. 2004. Molecular markers, natural history and evolution, 2nd edition. Sinauer Associates, Inc Publishers, Sunderland, Massachusetts.
4. Batalha, M. A., and W. Mantovani. 2000. Reproductive phenological patterns of cerrado plant species at the Pé-de-Gigante Reserve (Santa Rita do Passa Quatro, SP, Brazil): A comparison between the herbaceous and woody floras. Braz. J. Biol. 6 : 129 145.
5. Beier, P. 1993. Determining minimum habitat areas and habitat corridors for cougars. Conserv. Biol. 7 : 94 107.
6. Bellemain, E., J. E. Swenson, D. Tallmon, S. Brunberg, and P. Taberlet. 2005. Estimating population size of elusive animals with DNA from hunter-collected feces: Four methods for brown bears. Conserv. Biol. 19 : 150 161.
7. Camara, G., R. C. M. Souza, U. M. Freitas, and J. Garrido. 1996. SPRING: Integrating remote sensing and GIS by object-oriented data modeling. Comput. Graph. 20 : 395 403.
8. Chame, M. 2003. Terrestrial mammal feces: A morphometric summary and description. Mem. Inst. Oswaldo Cruz 98 : 71 94.
9. Ernest, H. B., M. C. T. Penedo, B. P. May, M. S. Syvanen, and W. M. Boyce. 2000. Molecular tracking of mountain lions in the Yosemite Valley region in California: Genetic analysis using microsatellites and faecal DNA. Mol. Ecol. 9 : 433 441.
10. Farrel, L. E., J. Roman, and M. E. Sunquist. 2000. Dietary separation of sympatric carnivores identified by molecular analysis of scats. Mol. Ecol. 9 : 1583 1590.
11. Fonseca, G. A. B., R. A. Mittermeier, R. B. Cavalcanti, and C. G. Mittermeier. 2000. Brazilian Cerrado. In R. A. Mittermeier, N. Myers, P. R. Gil, and C.G. Mittermeier (Eds Hotspots, Earth's biologically richest and most endangered terrestrial ecoregions, pp. 148 159. Conservation International, Chicago, Illinois.
12. Frantzen, M. A., J. B. Silk, J. W. H. Ferguson, R. K. Wayne, and M. H. Kohn. 1998. Empirical evaluation of preservation methods for fecal DNA. Mol. Ecol. 7 : 1423 1428.
13. Gagneux, P., C. Boesch, and D. S. Woodruff. 1997. Microsatellite scoring errors associated with noninvasive genotyping base don nuclear DNA amplified from shed hair. Mol. Ecol. 6 : 861 868.
14. Hall, T. A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41 : 95 98.
15. IUCN 2006. IUCN Red List of Threatened Species. IUCN, New York, New York.
16. Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. J. Mol. Evol. 16 : 111 120.
17. Kohn, M., and R. K. Wayne. 1997. Facts from feces revisited. Trends ecol. Evol. 12 : 223 227.
18. Kohn, M., F. Knauer, A. Stoffella, W. Schröder, and S. Pääbo. 1995. Conservation genetics of the European brown bear - A study using excremental PCR of nuclear and mitochondrial sequences. Mol. Ecol. 4 : 95 103.
19. Kohn, M., E. C. York, D. A. Kamradt, G. Haught, R. M. Sauvajot, and R. K. Wayne. 1999. Estimating population size by genotyping feces. Proc. R. Soc. Lond. B 266 : 657 663.
20. Korman, V. 2003. Proposal for the interconnection of the Vassununga State Park fragments, state of São Paulo, Brazil. Dissertation, Esalq - USP, Piracicaba, Brasil.
21. Kumar, S., K. M. Tamura, and M. Nei. 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief. Bioinform. 5 : 150 163.
22. Meffe, G. K., and C. R. Carroll. 1997. Principles of conservation biology, 2nd edition. Sinauer Associates, Sunderland, Massachusetts.
23. Menotti-Raymond, M. A., and S. J. O'Brien. 1995. Evolutionary conservation of ten microsatellite loci in four species of Felidae. J. Hered. 86 : 319 322.
24. Menotti-Raymond, M. A., V. A. David, L. A. Lyons, A. A. Schäffer, J. F. Tomlin, M. K. Hutton, and S. J. O'Brien. 1999. A genetic linkage map of microsatellites in the domestic cat (Felis catus). Genomics 57 : 9 23.
25. Mills, L. S., J. J. Citta, K. P. Lair, M. K. Schwartz, and D. A. Tallmon 2000. Estimating animal abundance using noninvasive DNA sampling: Promise and pitfalls. Ecol. Appl. 10 : 283 294.
26. Morin, P. A., and D. S. Woodruff. 1996. Noninvasive genotyping for vertebrate conservation. In T. B. Smith, and R. K. Wayne (Eds Molecular genetics approaches in conservation, pp. 298 313. Oxford University, New York, New York.
27. Morin, P. A., K. E. Chambers, C. Boesch, and L. Vigilant. 2001. Quantitative polymerase chain reaction analysis of DNA from noninvasive samples for accurate microsatellite genotyping of wild chimpanzees (Pan troglodytes verus). Mol. Ecol. 10 : 1835 1844.
28. Paetkau, D., L. P. Waits, P. L. Clarkson, L. Craibghead, E. Vyse, R. Ward, and C. Strobrck. 1998. Variation in genetic diversity across the range of North American brown bears. Conserv. Biol. 12 : 418 429.
29. Palomares, F., J. A. Godoi, A. Piriz, S. J. O'Brien, and W. E. Johnson. 2002. Fecal genetic analysis to determinate the presence and distribution of elusive carnivores: Design and feasibility for the Iberian lynx. Mol. Ecol. 11 : 2171 2182.
30. Perez-Sweeney, B. M., F. P. Rodrigues, and D. J. Melnick. 2003. Metodologias moleculares utilizadas em genética da conservaç ão. In L. Cullen Jr., R. Rudran, and C. Valladares-Padua (Eds Métodos de estudo em biologia da conservação e manejo da vida silvestre, pp. 343 380. Editora UFPR, Fundação O Boticário de Proteção à Natureza, Curitiba, Paraná, Brasil.
31. Prada, C. S. 2004. Atropelamento de vertebrados silvestres em uma região fragmentada do nordeste do estado de São Paulo: quantificação do impacto e análise de fatores envolvidos. Dissertação de Mestrado, Universidade Federal de São Carlos, São Carlos, Brasil.
32. Prugh, L. R., C. E. Ritland, M. A. Arthur, and C. J. Krebs. 2005. Monitoring coyote population dynamics by genotyping faeces. Mol. Ecol. 14 : 1585 1596.
33. Ratter, J. A., J. F. Ribeiro, and S. Bridgewater. 1997. The Brazilian cerrado vegetation and threats to its biodiversity. Ann. Bot. Lond. 80 : 223 230.
34. Ruth, T. K., K. A. Logan, L. Sweanor, M. G. Hornocker, and L. J. Temple. 1998. Evaluating cougar translocation in New Mexico. J. Wildl. Mgmt. 62 : 1264 1275.
35. Saitou, N., and M. Nei. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4 : 406 425.
36. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: A laboratory manual, 2nd edition. Cold Spring Harbor Press, New York, New York.
37. Schuelke, M. 2000. An economic method for the fluorescent labeling of PCR fragments. Nat. Biotechnol. 18 : 233 234.
38. Shaffer, M. 1987. Minimum viable populations: Coping with uncertainty. In M. E. Soulé (Ed Viable populations for conservation, pp. 69 86. Cambridge University Press, New York, New York.
39. Sweanor, L. L., K. A. Logan, and M. G. Hornocker. 2000. Cougar dispersal patterns, metapopulation dynamics, and conservation. Conserv. Biol. 14 : 798 808.
40. Taberlet, P., S. Griffin, B. Goossens, S. Questiau, V. Manceau, N. Escaravage, L. P. Waits, and J. Bouvet. 1996. Reliable genotyping of samples with very low DNA quantities using PCR. Nucleic Acids Res. 24 : 3189 3194.
41. Taberlet, P., L. P. Waits, and G. Luikart. 1999. Noninvasive genetic sampling: Look before you leap. Trends ecol. Evol. 14 : 323 327.
42. Talamoni, S. A., J. C. Motta Junior, and M. M. Dias. 2000. Fauna de mamíferos da Estação Ecológica de Jataí e da Estação Experimental de Luiz Antônio. In J. E. Santos and J. S. R. Pires (Eds Estudos integrados em ecossistemas, Estação Ecológica de Jataí, vol 1. Editora Rima, São Carlos, São Paulo, Brasil.
43. Thompson, J. D., T. J. Gibson, F. Plewniak, F. Jeanmougin, and D. G. Higgins. 1997. The Clustal X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 24 : 4876 4882.
44. Waits, L. P., G. Luikart, and P. Tarbelet. 2001. Estimating the probability of identity among genotypes in natural populations: Cautions and guidelines. Mol. Ecol. 10 : 249 256.
45. Wasser, S. K., G. M. Houston, G. G. Cadd, and R. Fain. 1997. Techniques for application of fecal DNA methods to field studies of Ursids. Mol. Ecol. 6 : 1091 1097.
46. Wayne, R. K., and P. A. Morin. 2004. Conservation genetics in the new molecular age. Front. Eco. Evrmt. 2 : 89 97.