Calibration-curve-free quantitative PCR: A quantitative method for specific nucleic acid sequences without using calibration curves

Analytical Biochemistry

Volumn 369, Issue 1, 2007, Pages 105-111

  Tani, Hidenori ^a,b   Kanagawa, Takahiro ^b,c   Morita, Nao ^a,b   Kurata, Shinya ^d   Nakamura, Kazunori ^b,e   Tsuneda, Satoshi ^a   Noda, Naohiro ^b  

^a WASEDA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c Kyoto University of Advanced Science   (Japan)

^d J Bio 21 Corporation   (Japan)

^e UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Competitive PCR; Fluorescence quenching; Genetically modified organisms; Quenching probe; Real time PCR; RRS

Indexed keywords

CALIBRATION; FLUORESCENCE; FLUORESCENCE QUENCHING; GENES; NUCLEIC ACID SEQUENCES; PROBES; QUENCHING;

COMPETITIVE PCR; COMPETITIVE POLYMERASE CHAIN REACTION; FLUORESCENCE INTENSITIES; GENETICALLY MODIFIED ORGANISMS; GENETICALLY MODIFIED SOYBEANS; INTERNAL STANDARDS; QUANTITATIVE METHOD; REAL-TIME PCR;

POLYMERASE CHAIN REACTION;

FLUORESCENT DYE; GUANINE; RECOMBINANT DNA;

ARTICLE; GENE AMPLIFICATION; GENETICALLY MODIFIED CROP; HYBRIDIZATION; NUCLEOTIDE SEQUENCE; PHOTOCHEMICAL QUENCHING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SOYBEAN;

GLYCINE MAX;

EID: 34548189670     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.06.047     Document Type: Article

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