Quantification of genetically modified soybean by quenching probe polymerase chain reaction

Journal of Agricultural and Food Chemistry

Volumn 53, Issue 7, 2005, Pages 2535-2540

  Tani, Hidenori ^a   Noda, Naohiro ^b   Yamada, Kazutaka ^c   Kurata, Shinya ^c   Tsuneda, Satoshi ^a   Hirata, Akira ^a   Kanagawa, Takahiro ^b  

^a WASEDA UNIVERSITY   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^c Kankyo Engineering Co Ltd   (Japan)

Author keywords

Fluorescence quenching; GMOs; QProbe PCR; Quenching probe; Real time PCR; Roundup Ready soybean

Indexed keywords

ACCURACY; ARTICLE; COST EFFECTIVENESS ANALYSIS; GENE PROBE; GENETICALLY MODIFIED CROP; INTERMETHOD COMPARISON; NONHUMAN; POLYMERASE CHAIN REACTION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; RECOMBINANT GENE; SENSITIVITY AND SPECIFICITY; SOYBEAN;

DNA PROBES; DNA, PLANT; DNA, RECOMBINANT; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOYBEANS;

GLYCINE MAX;

EID: 16344367551     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf048031r     Document Type: Article

References (33)

1. Regulation (EC) No. 258/97 of the European Parliament and the Council of 27 January 1997 concerning novel foods and novel food ingredients. Off. J. Eur. Commun. 1997, L 43, 1-7.
2. Regulation (EC) 18/2001 of the European Parliament and of environment of genetically modified organisms and repealing Council Directive 90/220/EEC-Commission Declaration. Off. J. Eur. Commun. 2001, L 106, 1-39.
3. Regulation (EC) No. 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. Off. J. Eur. Commun. 2003, L 268, 1-23.
4. Regulation (EC) No. 1830/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labeling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending directive 2001/18/EC, Off. J. Eur. Commun. 2003, L 268, 24-28.
5. Notification No. 1775 (June 10, 2000) Food and Marketing Bureau, Ministry of Agriculture, Forestry and Fisheries of Japan, Tokyo, Japan.
6. Hardegger, M.; Brodmann, P.; Herrmann, A. Quantitative detection of the 35S promoter and the NOS terminator using quantitative competitive PCR. Eur. Food Res. Technol. 1999, 209, 83-87.
7. Hübner, P.; Studer, E.; Lüthy, J. Quantitative competitive PCR for the detection of genetically modified organisms in food. Food Control 1999, 10, 353-358.
8. Hübner, P.; Studer, E.; Lüthy, J. Quantitation of genetically modified organisms in food. Nat. Biotechnol. 1999, 17, 1137-1138.
9. Hupfer, C.; Hotzel, H.; Sachse, K.; Moreano, F.; Engel, K. H. PCR-based quantification of genetically modified Bt maize: single-competitive versus dual-competitive approach. Eur. Food Res. Technol. 2000, 212, 95-99.
10. García-Cañas, V.; Cifuentes, A.; González, R. Quantitation of transgenic Bt event-176 maize using double quantitative competitive polymerase chain reaction and capillary gel electrophoresis laser-induced fluorescence. Anal. Chem. 2004, 76, 2306-2313.
11. Vaïtilingom, M.; Pijnenburg, H.; Gendre, F.; Brignon, P. Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods. J. Agric. Food Chem. 1999, 47, 5261-5266.
12. Wurz, A.; Bluth, A.; Zelts, P.; Pfeifer, C.; Willmund, R. Quantitative analysis of genetically modified organisms (GMO) in processed food by PCR-based methods. Food Control 1999, 10, 385-389.
13. Berdal, K. G.; Holst-Jensen, A. Roundup Ready soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses. Eur. Food Res. Technol. 2001, 213, 432-438.
14. Taverniers, I.; Windeis, P.; Bockstaele, E. V.; Loose, M. D. Use of cloned DNA fragments for event-specific quantification of genetically modified organisms in pure and mixed food products. Eur. Food Res. Technol. 2001, 213, 417-424.
15. Terry, C. F.; Harris, N. Event-specific detection of Roundup Ready Soya using two different real time PCR detection chemistries. Eur. Food Res. Technol. 2001, 213, 425-431.
16. Alary, R.; Serin, A.; Maury, D.; Jouira, H. B.; Sirven, J. P.; Gautier, M. F.; Joudrier, P. Comparison of simplex and duplex real-time PCR for the quantification of GMO in maize and soybean. Food Control 2002, 13, 235-244.
17. Kuribara, H.; Shindo, Y.; Matsuoka, T.; Takubo, K.; Futo, S.; Aoki, N.; Hirao, T.; Akiyama, H.; Goda, Y.; Toyoda, M.; Hino, A. Novel reference molecules for quantitation of genetically modified maize and soybean. J. AOAC Int. 2002, 85, 1077-1089.
18. Permingeat, H. R.; Reggiardo, M. I.; Vallejos, R. H. Detection and quantification of transgenes in grains by multiplex and real-time PCR. J. Agric. Food Chem. 2002, 50, 4431-4436.
19. Shindo, Y.; Kuribara, H.; Matsuoka, T.; Futo, S.; Sawada, C.; Shono, J.; Akiyama, H.; Goda, Y.; Toyoda, M.; Hino, A. Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. J. AOAC Int. 2002, 85, 1119-1126.
20. Pardigol, A.; Guillet, S.; Popping, B. A simple procedure for quantification of genetically modified organisms using hybrid amplicon standards. Eur. Food Res. Technol. 2003, 216, 412-420.
21. Taverniers, I.; Bockstaele, E. V.; Loose, M. D. Cloned plasmid DNA fragments as calibrators for controlling GMOs: different real-time duplex quantitative PCR methods. Anal. Bioanal. Chem. 2004, 378, 1198-1207.
22. Höhne, M.; Santisi, C. R.; Meyer, R. Real-time multiplex PCR: An accurate method for the detection and quantification of 35S-CaMV promoter in genetically modified maize-containing food. Eur. Food Res. Technol. 2002, 215, 59-64.
23. Roønning, S. B.; Vaïtilingom, M.; Berdal, K. G.; Holst-Jensen, A. Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays). Eur. Food Res. Technol. 2003, 216, 347-354.
24. Holck, A.; Va, M.; Didierjean, L.; Rudi, K. 5′-nuclease PCR for quantitative event-specific detection of the genetically modified Mon810 MaisGard maize. Eur. Food Res. Technol. 2002, 214, 449-453.
25. Huang, H. Y.; Pan, T. M. Detection of genetically modified maize MON810 and NK603 by multiplex and real-time polymerase chain reaction methods. J. Agric. Food Chem. 2004, 52, 3264-3268.
26. Ministry of Health, Labour and Welfare, Japan. Testing for Foods Produced by Recombinant DNA Techniques, http://www.mhlw.go.jp/topics/idenshi/kensa/pdf/ tuuchi2.pdf.
27. Kurata, S.; Kanagawa, T.; Yamada, K.; Torimura, M.; Yokomaku, T.; Kamagata, Y.; Kurane, R. Fluorescent quenching-based quantitative detection of specific DNA/RNA using a BODIPY FL-labeled probe or primer. Nucleic Acids Res. 2001, 29, e34.
28. Crockett, A. O.; Wittwer, C. T. Fluorescein-labeled oligonucleotides for real-time PCR: using the inherent quenching of deoxyguanosine nucleotides. Anal. Biochem. 2001, 290, 89-97.
29. Torimura, M.; Kurata, S.; Yamada, K.; Yokomaku, T.; Kamagata, Y.; Kanagawa, T.; Kurane, R. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal. Sci. 2001, 17, 155-160.
30. Tyagi, S.; Kramer, F. R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 1996, 14, 303-308.
31. Wittwer, C. T.; Herrmann, M. G.; Moss, A. A.; Rasmussen, R. P. Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques 1997, 22, 130-138.
32. Nazarenko, I. A.; Bhatnagar, S. K.; Hohman, R. J. A closed tube format for amplification and detection of DNA based on energy transfer. Nucleic Acids Res. 1997, 25, 2516-2521.
33. Whitcombe, D.; Theaker, J.; Guy, S. P.; Brown, T.; Little, S. Detection of PCR products using self-probing amplicons and fluorescence. Nat. Biotechnol. 1999, 17, 804-807.