Glutamate recognition and hydride transfer by Escherichia coli glutamyl-tRNA reductase

FEBS Journal

Volumn 274, Issue 17, 2007, Pages 4609-4614

  Lüer, Corinna ^a   Schauer, Stefan ^b   Virus, Simone ^a   Schubert, Wolf Dieter ^c   Heinz, Dirk W ^c   Moser, Jürgen ^a   Jahn, Dieter ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b ETH ZURICH   (Switzerland)

^c HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

Escherichia coli; Glutamyl tRNA; Glutamyl tRNA reductase; Substrate recognition; Tetrapyrrole biosynthesis

Indexed keywords

AMINO ACID; ARGININE; CYSTEINE; ENZYME; ENZYME VARIANT; ESTER; GLUTAMIC ACID; GLUTAMYL TRANSFER RIBONUCLEIC ACID REDUCTASE; HISTIDINE; LEUCINE; LYSINE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; THIOESTER; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESCHERICHIA COLI; IN VITRO STUDY; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN STABILITY; PROTEIN STRUCTURE;

ALDEHYDE OXIDOREDUCTASES; BASE SEQUENCE; CATALYSIS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA PRIMERS; ESCHERICHIA COLI; GLUTAMIC ACID; HYDROGEN; KINETICS; MUTAGENESIS, SITE-DIRECTED;

ESCHERICHIA COLI;

EID: 34548138934     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.05989.x     Document Type: Article

References (22)

1. Shemin D Russell CS (1953) δ-aminolevulinic acid, its role in the biosynthesis of porphyrins and purines. J Am Chem Soc 75, 4873 4875.
2. Neuberger A Scott JJ (1953) Aminolaevulinic acid and porphyrin biosynthesis. Nature 172, 1093 1094.
3. Avissar YJ, Ormerod JG Beale SI (1989) Distribution of δ-aminolevulinic acid biosynthetic pathways among phototrophic bacterial groups. Arch Microbiol 151, 513 519.
4. Kikuchi G, Kumar A, Talmage P Shemin D (1958) The enzymatic synthesis of δ-aminolevulinic acid. J Biol Chem 233, 1214 1219.
5. 14C-δ- aminolevulinic acid from labeled precursors in greening plant tissues. Plant Physiol 53, 297 303.
6. Schön A, Krupp G, Gough S, Berry-Lowe S, Kannangara CG Söll D (1986) The RNA required in the first step of chlorophyll biosynthesis is a chloroplast glutamate tRNA. Nature 322, 281 284.
7. Jahn D, Verkamp E Söll D (1992) Glutamyl-transfer RNA: a precursor of heme and chlorophyll biosynthesis. Trends Biochem Sci 17, 215 218.
8. Al-Karadaghi S, Kristensen O Liljas A (2000) A decade of progress in understanding the structural basis of protein synthesis. Prog Biophys Mol Biol 73, 167 193.
9. Smith MA, Kannangara CG, Grimm B von Wettstein D (1991) Characterization of glutamate-1-semialdehyde aminotransferase of Synechococcus. Steady-state kinetic analysis. Eur J Biochem 202, 749 757.
10. Ilag LL Jahn D (1992) Activity and spectroscopic properties of the Escherichia coli glutamate 1-semialdehyde aminotransferase and the putative active site mutant K265R. Biochemistry 31, 7143 7151.
11. Glu reductase in Escherichia coli as a fusion protein with glutathione S-transferase. Proc Natl Acad Sci USA 93, 9287 9291.
12. Moser J, Lorenz S, Hubschwerlen C, Rompf A Jahn D (1999) Methanopyrus kandleri glutamyl-tRNA reductase. J Biol Chem 274, 30679 30685.
13. Moser J, Schubert WD, Beier V, Bringemeier I, Jahn D Heinz DW (2001) V-shaped structure of glutamyl-tRNA reductase, the first enzyme of tRNA-dependent tetrapyrrole biosynthesis. EMBO J 20, 6583 6590.
14. Schauer S, Chaturvedi S, Randau L, Moser J, Kitabatake M, Lorenz S, Verkamp E, Schubert WD, Nakayashiki T, Murai M et al. (2002) Escherichia coli glutamyl-tRNA reductase. Trapping the thioester intermediate. J Biol Chem 277, 48657 48663.
15. Srivastava A, Lake V, Nogaj LA, Mayer SM, Willows RD Beale SI (2005) The Chlamydomonas reinhardtii gtr gene encoding the tetrapyrrole biosynthetic enzyme glutamyl-tRNA reductase: structure of the gene and properties of the expressed enzyme. Plant Mol Biol 58, 643 658.
16. Randau L, Schauer S, Ambrogelly A, Salazar JC, Moser J, Sekine S, Yokoyama S, Söll D Jahn D (2004) tRNA recognition by glutamyl-tRNA reductase. J Biol Chem 279, 34931 34937.
17. Lüer C, Schauer S, Möbius K, Schulze J, Schubert WD, Heinz DW, Jahn D Moser J (2005) Complex formation between glutamyl-tRNA reductase and glutamate-1-semialdehyde 2,1-aminomutase in Escherichia coli during the initial reactions of porphyrin biosynthesis. J Biol Chem 280, 18568 18572.
18. Nogaj LA, Srivastava A, van Lis R Beale SI (2005) Cellular levels of glutamyl-tRNA reductase and glutamate-1-semialdehyde aminotransferase do not control chlorophyll synthesis in Chlamydomonas reinhardtii. Plant Physiol 139, 389 396.
19. Srivastava A Beale SI (2005) Glutamyl-tRNA reductase of Chlorobium vibrioforme is a dissociable homodimer that contains one tightly bound heme per subunit. J Bacteriol 187, 4444 4450.
20. Schauer S, Lüer C Moser J (2003) Large scale production of biologically active Escherichia coli glutamyl-tRNA reductase from inclusion bodies. Protein Expr Purif 31, 271 275.
21. Lin SX, Brisson A, Liu J, Roy PH Lapointe J (1992) Higher specific activity of the Escherichia coli glutamyl-tRNA synthetase purified to homogeneity by a six-hour procedure. Protein Expr Purif 3, 71 74.
22. Eisenthal R Danson MJ (2002) Enzyme Assays: A Practical Approach, 2nd edn, pp. 18 19. Oxford University Press, Oxford.