Alternative methods for genetic transformation of Pseudozyma antarctica, a basidiomycetous yeast-like fungus

Journal of Microbiological Methods

Volumn 70, Issue 3, 2007, Pages 519-527

  Marchand, G ^a   Fortier, E ^a   Neveu, B ^a   Bolduc, S ^a   Belzile, F ^a   Belanger R R ^a  

^a UNIVERSITÉ LAVAL   (Canada)

Author keywords

Agrobacterium tumefaciens; Electroporation; Genetic transformation; Pseudozyma antarctica

Indexed keywords

CALCIUM CHLORIDE; GREEN FLUORESCENT PROTEIN; HYDROLASE; HYGROMYCIN;

ANALYTIC METHOD; ARTICLE; BASIDIOMYCETES; CELL WALL; CONIDIUM; CONTROLLED STUDY; CULTURE MEDIUM; ELECTROPORATION; GENE INSERTION; GENETIC TRANSFORMATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTOPLAST; PSEUDOZYMA ANTARTICA; RHIZOBIUM RADIOBACTER;

BASIDIOMYCOTA; ELECTROPORATION; RHIZOBIUM RADIOBACTER; SPORES, FUNGAL; TRANSFORMATION, GENETIC;

AGROBACTERIUM TUMEFACIENS; FUNGI; PSEUDOZYMA ANTARCTICA;

EID: 34548120570     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2007.06.014     Document Type: Article

References (38)

1. Avis T.J., Cheng Y.L., Zhao Y.Y., Bolduc S., Neveu B., Anguenot R., Labbé C., Belzile F., and Bélanger R.R. The potential of Pseudozyma yeast-like epiphytes for the production of heterologous recombinant proteins. Appl. Microbiol. Biotechnol. 69 (2005) 304-311
2. Becker D.M., and Guarente L. High-efficiency transformation of yeast by electroporation. Methods Enzymol. 194 (1991) 182-187
3. Bélanger R.R., and Avis T.J. Ecological processes and interactions occurring in leaf surface fungi. In: Lindow S.E., Hecht-Poinar E.I., and Elliott V.J. (Eds). Phyllosphere Microbiology (2002), APS Press, St-Paul, MN 193-207
4. Boekhout T. Pseudozyma Bandoni emend Boekhout, a genus for yeast-like anamorphs of Ustilaginales. J. Gen. Appl. Microbiol. 41 (1995) 359-366
5. Brown L.E., Sprecher S.L., and Keller L.R. Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation. Mol. Cell. Biol. 11 (1991) 2328-2332
6. Brown J.S., Aufauvre-Brown A., and Holden D.W. Insertional mutagenesis of Aspergillus fumigatus. Mol. Gen. Genet. 259 (1998) 327-335
7. Bruns T. Evolutionary biology: a kingdom revised. Nature 443 (2006) 758-761
8. Bundock P., den Dulk-Ras A., Beijersbergen A., and Hooykaas P.J.J. Trans-kingdom T-DNA transfer from Agrobacterium tumefaciens to Saccharomyces cerevisiae. EMBO J. 14 (1995) 3206-3214
9. Chakraborty B.N., and Kapoor M. Transformation of filamentous fungi by electroporation. Nucleic Acids Res. 18 (1990) 6737
10. Chakraborty B.N., Patterson N.A., and Kapoor M. An electroporation-based system for high-efficiency transformation of germinated conidia of filamentous fungi. Can. J. Microbiol. 37 (1991) 858-863
11. Chang D.C. Structure and dynamics of electric field-induced membrane pores as revealed by rapid-freezing electron microscopy. In: Chang D.C., Chassy B.M., Saunders J.A., and Sowers A.E. (Eds). Guide to Electroporation and Electrofusion (1992), Academic Press Inc., San Diego, CA 9-27
12. Cheng, Y.L., 2005. (Université Laval.) Personal communication.
13. Cheng Y.L., and Bélanger R.R. Protoplast preparation and regeneration from spores of the biocontrol fungus Pseudozyma flocculosa. FEMS Microbiol. Lett. 190 (2000) 287-291
14. Cheng Y.L., Belzile F., Tanguay P., Bernier L., and Bélanger R.R. Establishment of a gene transfer system for Pseudozyma flocculosa, an antagonistic fungus of powdery mildew fungi. Mol. Genet. Genomics 266 (2001) 96-102
15. Covert S.F., Kapoor P., Lee M., Briley A., and Nairn C.J. Agrobacterium tumefaciens-mediated transformation of Fusarium circinatum. Mycol. Res. 105 (2001) 259-264
16. Dantas-Barbosa C., Araújo E.F., Moraes L.M.P., Vainstein M.H., and Azevedo M.O. Genetic transformation of germinated conidia of the thermophilic fungus Humicola grisea var. thermoidea to hygromycin B resistance. FEMS Microbiol. Lett. 169 (1998) 185-190
17. Davidson M.K., Young N.P., Glick G.G., and Wahls W.P. Meiotic chromosome segregation mutants identified by insertional mutagenesis of fission yeast Schizosaccharomyces pombe; tandem-repeat, single-site integrations. Nucleic Acids Res. 32 (2004) 4400-4410
18. de Groot M.J.A., Bundock P., Hooykaas P.J.J., and Beijersbergen A.G. Agrobacterium tumefaciens-mediated transformation of filamentous fungi. Nat. Biotechnol. 6 (1998) 839-842
19. Gácser A., Salomon S., and Schäfer W. Direct transformation of a clinical isolate of Candida parapsilosis using a dominant selection marker. FEMS Microbiol. Lett. 245 (2005) 117-121
20. Gelvin S.B. Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool. Microbiol. Mol. Biol. Rev. 67 (2003) 16-37
21. Godio R.P., Fouces R., Gudina E.J., and Martin J.F. Agrobacterium tumefaciens-mediated transformation of the antitumor clavaric acid-producing basidiomycete Hypholoma sublateritium. Curr. Genet. 46 (2004) 287-294
22. Hashimoto H., Morikawa H., Yamada Y., and Kimura A. A novel method for transformation of intact yeast-cells by electroinjection of plasmid DNA. Appl. Microbiol. Biotechnol. 21 (1985) 336-339
23. Karube I., Tamiya E., and Matsuoka H. Transformation of Saccharomyces cerevisiae spheroplasts by high electric pulse. FEBS Lett. 182 (1985) 90-94
24. Kononov M.E., Bassuner B., and Gelvin S.B. Integration of T-DNA binary vector "backbone" sequences into the tobacco genome: evidence for multiple complex patterns of integration. Plant J. 11 (1997) 945-957
25. Kuo C.Y., Chou S.Y., and Huang C.T. Cloning of glyceraldehyde-3-phosphate dehydrogenase gene and use of the gpd promoter for transformation in Flammulina velutipes. Appl. Microbiol. Biotechnol. 65 (2004) 593-599
26. Martineau B., Voelker T.A., and Sanders R.A. On defining T-DNA. Plant Cell 6 (1994) 1032-1033
27. Michielse C.B., Hooykaas P.J.J., van den Hondel C.A.M.J.J., and Ram A.F.J. Agrobacterium-mediated transformation as a tool for functional genomics in fungi. Curr. Genet. 48 (2005) 1-17
28. Richey M.G., Marek E.T., Schardl C.L., and Smith D.A. Transformation of filamentous fungi with plasmid DNA by electroporation. Phytopathology 79 (1989) 844-847
29. Robinson M., and Sharon A. Transformation of the bioherbicide Colletotrichum gloeosporioides f. sp. aeschynomene by electroporation of germinated conidia. Curr. Genet. 36 (1999) 98-104
30. Saito T., Tanaka N., and Shinozawa T. A transformation system for an ectomycorrhizal basidiomycete, Lyophyllum shimeji. Biosci. Biotechnol. Biochem. 65 (2001) 1928-1931
31. Sambrook J., Fritsh E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual (1989), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
32. Sánchez O., and Aguirre J. Efficient transformation of Aspergillus nidulans by electroporation of germinated conidia. Fungal Genet. Newslett. 43 (1996) 48-51
33. Suga M., and Hatakeyama T. High-efficiency electroporation by freezing intact yeast cells with addition of calcium. Curr. Genet. 43 (2003) 206-211
34. Vertesy, L., Kurz, M., Wink, J., Noelken, G., 2002. Ustilipides, method for the production and the use thereof. US Patent 6,472,158.
35. Weaver J.C. Electroporation theory: concepts and mechanisms. In: Nickoloff J. (Ed). Electroporation Protocols for Microorganisms (1995), Humana Press, Totowa, NJ 1-26
36. Weidner G., d'Enfert C., Koch A., Pieternella C.M., and Brakhage A.A. Development of a homologous transformation system for the human pathogenic fungus Aspergillus fumigatus based on the pyrG gene encoding orotidine 5'-monophosphate decarboxylase. Curr. Genet. 33 (1998) 378-385
37. Wenck A., Czako M., Kanevski I., and Marton L. Frequent collinear long transfer of DNA inclusive of the whole binary vector during Agrobacterium-mediated transformation. Plant Mol. Biol. 34 (1997) 913-922
38. Yehuda H., Droby S., Wisniewski M., and Goldway M. A transformation system for the biocontrol yeast, Candida oleophila, based on hygromycin B resistance. Curr. Genet. 40 (2001) 282-287