A transformation system for an ectomycorrhizal basidiomycete, Lyophyllum shimeji

Bioscience, Biotechnology and Biochemistry

Volumn 65, Issue 8, 2001, Pages 1928-1931

  Saito, Takeshi ^a,b   Tanaka, Norio ^b   Shinozawa, Takao ^a  

^a GUNMA UNIVERSITY   (Japan)

^b Mushroom Research Institute of Japan   (Japan)

Author keywords

Ectomycorrhizal basidiomycete; Hygromycin B; Lyophyllum shimeji; Transformation

Indexed keywords

BASIDIOMYCOTA; ESCHERICHIA COLI; LYOPHYLLUM SHIMEJI;

FUNGAL DNA; HYGROMYCIN B KINASE; HYGROMYCIN-B KINASE; PHOSPHOTRANSFERASE;

ARTICLE; BASIDIOMYCETES; CULTURE MEDIUM; ESCHERICHIA COLI; FUNGAL GENE; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; METABOLISM; MICROBIOLOGY; PHYSIOLOGY; PLASMID; SOUTHERN BLOTTING; SYMBIOSIS; TREE;

BASIDIOMYCOTA; BLOTTING, SOUTHERN; CULTURE MEDIA; DNA, FUNGAL; ESCHERICHIA COLI; GENES, FUNGAL; GENETIC VECTORS; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PLASMIDS; SYMBIOSIS; TRANSFORMATION, GENETIC; TREES;

EID: 0035430481     PISSN: 09168451     EISSN: None     Source Type: Journal    
DOI: 10.1271/bbb.65.1928     Document Type: Article

References (17)

1. Harley, J. L. and Smith, S. E., Mycorrhizal symbiosis. Academic Press, London, pp. 483 (1983).
2. Smith, S. and Read, D. J., Mycorrhizal symbiosis. Academic Press, London, pp. 640 (1996).
3. Ohta, A., Some cultural characteristics of mycelia of a mycorrhizal fungus, Lyophyllum shimeji. Mycoscience, 35, 83-87 (1994).
4. Ogawa, M., Matsutake no seibutugaku (in Japanese). Tsukiji Shokan, Tokyo, pp. 326 (1978).
5. Kawai, M., Artificial ectomycorrhiza formation on roots of air-layered Pinus densiflora saplings by inoculation with Lyophyllum shimeji. Mycologia, 89, 228-232 (1997).
6. Barrett, V., Dixon, R. K., and Lemke, P. A., Genetic transformation of a mycorrhizal fungus. Appl. Microbiol. Biotechnol., 33, 313-316 (1990).
7. Marmeisse, R., Gay, G., Debaud, J.-C., and Casselton, L. A., Genetic transformation of the symbiotic basidiomycete fungus Hebeloma cylindrosporum. Curr. Genet., 22, 41-45 (1992).
8. Bills, S., Podila, G. K., and Hiremath, S., Genetic engineering of an ectomycorrhizal fungus Laccaria bicolor for use as a biological control agent. Mycologia, 91, 237-242 (1999).
9. Saito, T. and Tanaka, N., Cloning and sequence analysis of the glyceraldehyde-3-phosphate dehydrogenase gene from the ectomycorrhizal basidiomycete Lyophyllum shimeji. Mycoscience, 40, 517-523 (1999).
10. Jurand, A. and Goel, S. C., The use of methyl green-pyronin staining after glutaraldehyde fixation and paraffin or araldite embedding. Tissue Cell, 8, 389-94 (1976).
11. Freudenberg, S., Fasold, K.-I., Mueller, S. R., Siedenberg, D., Kretzmer, G., Schuegerl, K., and Guiseppi, N. M., Fluorescence microscopic investigation of Aspergillus awamori growing on synthetic and complex media and producing xylanase. J. Biotechnol., 46, 265-273 (1996).
12. Murray, M. G. and Thompson, W. F., Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res., 8, 4321-4325 (1980).
13. Southern, E. M., Detection of specific sequence among DNA fragments separated by gel electrophoresis. J. Mol. Biol., 98, 503-517 (1975).
14. Schuren, F. H. J. and Wessels, J. G. H., High-efficient transformation of the homobasidiomycete Schizophyllum commune to phleomycin resistance. Curr. Genet., 26, 179-183 (1994).
15. Van de Rhee, M. D., Graça, P. M. A., Huizing, H. J., and Mooibroek, H., Transformation of the cultivated mushroom, Agaricus bisporus, to hygromycin B resistance. Mol. Gen. Genet., 250, 252-258 (1996).
16. Yanai, K., Yonekura, K., Usami, H., Hirayama, M., Kajiwara, S., Yamazaki, T., Shishido, K., and Adachi, T., The integrative transformation of Pleurotus ostreatus using bialaphos resistance as a dominant selective marker. Biosci. Biotechnol. Biochem., 60, 472-475 (1996).
17. Sambrook, J., Fritsch, E. F., and Maniatis, T., "Molecular cloning: a laboratory mannual", 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y. (1989).