A flow cytometry technique to study intracellular signals NF-κB and STAT3 in peripheral blood mononuclear cells

BMC Molecular Biology

Volumn 8, Issue , 2007, Pages

  Lafarge, Sandrine ^a,b   Hamzeh Cognasse, Hind ^b   Chavarin, Patricia ^a   Genin, Christian ^b   Garraud, Olivier ^a,b   Cognasse, Fabrice ^a,b  

^a ETABLISSEMENT FRANÇAIS DU SANG   (France)

^b UNIVERSITÉ DE LYON   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CD40 LIGAND; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERLEUKIN 10; INTERLEUKIN 1BETA; STAT3 PROTEIN;

ARTICLE; B LYMPHOCYTE; CELL COMMUNICATION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MACROPHAGE; MONOCYTE; PERIPHERAL BLOOD MONONUCLEAR CELL; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; T LYMPHOCYTE; WESTERN BLOTTING; CELL CULTURE; CYTOLOGY; GENETICS; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; METABOLISM; METHODOLOGY; MONONUCLEAR CELL; PHYSIOLOGY;

B-LYMPHOCYTES; CD40 LIGAND; CELLS, CULTURED; FLOW CYTOMETRY; HUMANS; INTERLEUKIN-10; INTERLEUKIN-1BETA; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE ACTIVATION; MACROPHAGES; NF-KAPPA B; SIGNAL TRANSDUCTION; STAT3 TRANSCRIPTION FACTOR; T-LYMPHOCYTES;

EID: 34548084513     PISSN: None     EISSN: 14712199     Source Type: Journal    
DOI: 10.1186/1471-2199-8-64     Document Type: Article

References (37)

1. Shuai K Liu B Regulation of JAK-STAT signalling in the immune system Nat Rev Immunol 2003 3 900-911 10.1038/nri1226 14668806
2. Bonizzi G Karin M The two NF-kappaB activation pathways and their role in innate and adaptive immunity Trends Immunol 2004 25 280-288 10.1016/ j.it.2004.03.008 15145317
3. Bottex-Gauthier C Pollet S Favier A Vidal DR The Rel/NF-kappa-B transcription factors: Complex role in cell regulation Pathol Biol (Paris) 2002 50 204-211 11980335
4. Cognasse F Sabido O Beniguel L Genin C Garraud O A flow cytometry technique to study nuclear factor-kappa B (NFkappaB) translocation during human B cell activation Immunol Lett 2003 90 49-52 10.1016/ S0165-2478(03)00173-1 14611907
5. Hoffmann A Baltimore D Circuitry of nuclear factor kappaB signaling Immunol Rev 2006 210 171-186 10.1111/j.0105-2896.2006.00375.x 16623771
6. Pomerantz JL Baltimore D Two pathways to NF-kappaB Mol Cell 2002 10 693-695 10.1016/S1097-2765(02)00697-4 12419209
7. Romieu-Mourez R Landesman-Bollag E Seldin DC Traish AM Mercurio F Sonenshein GE Roles of IKK kinases and protein kinase CK2 in activation of nuclear factor-kappaB in breast cancer Cancer Res 2001 61 3810-3818 11325857
8. Robe PA Bentires-Alj M Bonif M Rogister B Deprez M Haddada H Khac MT Jolois O Erkmen K Merville MP Black PM Bours V In vitro and in vivo activity of the nuclear factor-kappaB inhibitor sulfasalazine in human glioblastomas Clin Cancer Res 2004 10 5595-5603 10.1158/ 1078-0432.CCR-03-0392 15328202
9. Gasparian AV Yao YJ Kowalczyk D Lyakh LA Karseladze A Slaga TJ Budunova IV The role of IKK in constitutive activation of NF-kappaB transcription factor in prostate carcinoma cells J Cell Sci 2002 115 141-151 11801732
10. Biswas DK Shi Q Baily S Strickland I Ghosh S Pardee AB Iglehart JD NF-kappa B activation in human breast cancer specimens and its role in cell proliferation and apoptosis Proc Natl Acad Sci U S A 2004 101 10137-10142 454178 15220474 10.1073/pnas.0403621101
11. Viatour P Merville MP Bours V Chariot A Phosphorylation of NF-kappaB and IkappaB proteins: Implications in cancer and inflammation Trends Biochem Sci 2005 30 43-52 10.1016/j.tibs.2004.11.009 15653325
12. Schindler CW Series introduction. JAK-STAT signaling in human disease J Clin Invest 2002 109 1133-1137 150971 11994400 10.1172/JCI200215644
13. O'Shea J J Targeting the Jak/STAT pathway for immunosuppression Ann Rheum Dis 2004 63 Suppl 2 ii67-ii71 10.1136/ard.2004.028290 15479876
14. Ivashkiv LB Jak-STAT signaling pathways in cells of the immune system Rev Immunogenet 2000 2 220-230 11258419
15. Tsuyama N Danjoh I Otsuyama K Obata M Tahara H Ohta T Ishikawa H IL-6-induced Bcl6 variant 2 supports IL-6-dependent myeloma cell proliferation and survival through STAT3 Biochem Biophys Res Commun 2005 337 201-208 10.1016/j.bbrc.2005.09.036 16188230
16. Hannah AL Kinases as drug discovery targets in hematologic malignancies Curr Mol Med 2005 5 625-642 10.2174/156652405774641106 16305489
17. Gibbs CP Kukekov VG Reith JD Tchigrinova O Suslov ON Scott EW Ghivizzani SC Ignatova TN Steindler DA Stem-like cells in bone sarcomas: implications for tumorigenesis Neoplasia 2005 7 967-976 1502023 16331882 10.1593/neo.05394
18. Alas S Bonavida B Inhibition of constitutive STAT3 activity sensitizes resistant non-Hodgkin's lymphoma and multiple myeloma to chemotherapeutic drug-mediated apoptosis Clin Cancer Res 2003 9 316-326 12538484
19. Brenne AT Ro TB Waage A Sundan A Borset M Hjorth-Hansen H Interleukin-21 is a growth and survival factor for human myeloma cells Blood 2002 99 3756-3762 10.1182/blood.V99.10.3756 11986233
20. Dimberg LY Dimberg AI Ivarsson K Stromberg T Osterborg A Nilsson K Oberg F Jernberg Wiklund H Ectopic and IFN-induced expression of Fas overcomes resistance to Fas-mediated apoptosis in multiple myeloma cells Blood 2005 106 1346-1354 10.1182/blood-2004-04-1322 15860671
21. Miettinen M Lehtonen A Julkunen I Matikainen S Lactobacilli and Streptococci activate NF-kappa B and STAT signaling pathways in human macrophages J Immunol 2000 164 3733-3740 10725732
22. Zhang L Hong K Zhang J Pagano JS Multiple signal transducers and activators of transcription are induced by EBV LMP-1 Virology 2004 323 141-152 10.1016/j.virol.2004.03.007 15165826
23. Fumimori T Honda S Migita K Hamada M Yoshimuta T Honda J Fukuda T Suzuki R Gotoh M Eguchi K Aizawa H Erythromycin suppresses the expression of cyclooxygenase-2 in rheumatoid synovial cells J Rheumatol 2004 31 436-441 14994385
24. Strengell M Matikainen S Siren J Lehtonen A Foster D Julkunen I Sareneva T IL-21 in synergy with IL-15 or IL-18 enhances IFN-gamma production in human NK and T cells J Immunol 2003 170 5464-5469 12759422
25. Kato H Inoue T Asanoma K Matsuda T Yoshikawa Y Wake N Activation of STAT3/5 signal pathways in complete mole and repression in choriocarcinoma cell lines J Reprod Med 2006 51 41-48 16482776
26. Godoy-Tundidor S Cavarretta IT Fuchs D Fiechtl M Steiner H Friedbichler K Bartsch G Hobisch A Culig Z Interleukin-6 and oncostatin M stimulation of proliferation of prostate cancer 22Rv1 cells through the signaling pathways of p38 mitogen-activated protein kinase and phosphatidylinositol 3-kinase Prostate 2005 64 209-216 10.1002/ pros.20235 15712220
27. Blaecke A Delneste Y Herbault N Jeannin P Bonnefoy JY Beck A Aubry JP Measurement of nuclear factor-kappa B translocation on lipopolysaccharide-activated human dendritic cells by confocal microscopy and flow cytometry Cytometry 2002 48 71-79 10.1002/cyto.10115 12116367
28. Cognasse F Chavarin P Acquart S Sabido O Beniguel L Genin C Richard Y Garraud O Differential downstream effects of CD40 ligation mediated by membrane or soluble CD40L and agonistic Ab: A study on purified human B cells Int J Immunopathol Pharmacol 2005 18 65-74 15698512
29. Nelson DE Ihekwaba AE Elliott M Johnson JR Gibney CA Foreman BE Nelson G See V Horton CA Spiller DG Edwards SW McDowell HP Unitt JF Sullivan E Grimley R Benson N Broomhead D Kell DB White MR Oscillations in NF-kappaB signaling control the dynamics of gene expression Science 2004 306 704-708 10.1126/science.1099962 15499023
30. Perez OD Nolan GP Phospho-proteomic immune analysis by flow cytometry: from mechanism to translational medicine at the single-cell level Immunol Rev 2006 210 208-228 10.1111/j.0105-2896.2006.00364.x 16623773
31. Maes ML Davidson LB McDonagh PF Ritter LS Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry J Immunol Methods 2007 319 79-86 1896319 17187818 10.1016/j.jim.2006.10.016
32. Husain Z Holodick N Day C Szymanski I Alper CA Increased Apoptosis of CD20(+) IgA (+) B cells is the basis for IgA deficiency: The molecular mechanism for correction in vitro by IL-10 and CD40L J Clin Immunol 2006 26 113-125 10.1007/s10875-006-9001-y 16758339
33. Li X Fang Y Zhao X Jiang X Duong T Kain SR Characterization of NFkappaB activation by detection of green fluorescent protein-tagged IkappaB degradation in living cells J Biol Chem 1999 274 21244-21250 10.1074/ jbc.274.30.21244 10409681
34. Karin M Nuclear factor-kappaB in cancer development and progression Nature 2006 441 431-436 10.1038/nature04870 16724054
35. Vakkila J DeMarco RA Lotze MT Imaging analysis of STAT1 and NF-kappaB translocation in dendritic cells at the single cell level J Immunol Methods 2004 294 123-134 10.1016/j.jim.2004.09.007 15604022
36. Brunet A Signal transduction from the membrane to the nucleus: variations on common themes Bull Cancer 1998 85 527-537 9752280
37. Fanslow WC Anderson DM Grabstein KH Clark EA Cosman D Armitage RJ Soluble forms of CD40 inhibit biologic responses of human B cells J Immunol 1992 149 655-660 1378075