Bi-functional activities of chimeric lysozymes constructed by domain swapping between bacteriophage T7 and K11 lysozymes

Journal of Biochemistry and Molecular Biology

Volumn 40, Issue 4, 2007, Pages 539-546

  Alcantara, Ethel H ^a   Dong, Hee Kim ^a   Do, Su Il ^b   Sang, Soo Lee ^a  

^a PaiChai University   (South Korea)

^b Ajou University   (South Korea)

Author keywords

Amidase; His tagging; K11 RNA polymerase; Phage K11 lysozyme; Transcription inhibition

Indexed keywords

EID: 34548042328     PISSN: 12258687     EISSN: 02191024     Source Type: Journal    
DOI: 10.5483/bmbrep.2007.40.4.539     Document Type: Article

References (20)

1. Berger, N., Heller, A. E., Stormann, K. D. and Pfaff, E. (2001) Characterization of chimeric enzymes between caprine arthritis-encephaltis virus, maedi-visna virus and human immunodeficiency virus type 1 intergrases expressed in E. coli. J. Gen. Virol. 82, 139-148.
2. Brown, J. E., Klement, J. F. and MacAllister, W. T. (1986) Sequences of three promoters for the bacteriophage SP6 RNA polymerase. Nucleic Acids Res. 14, 3521-3526.
3. Chamberlin, M., McGrath, J. and Waskell, L. (1970) New RNA polymerase from Escherichia coli infected with baceriophage T7. Nature 228, 227-231.
4. Cheng, X., Zhang, X., Pflugrath, J. W. and Studier, F. W. (1993) The structure of bacteriophage T7 lysozyme, a zinc amidase and an inhibitor of T7 RNA polymerase. Proc. Natl. Acad. Sci. 91, 4034-4038.
5. Clark, E. D. (1998) Refolding of recombinant proteins. Curr. Opinion Biotech. 9, 157-163.
6. Dietz, A., Weisser, H.-J., Kössel, H. and Hausmann, R. (1990) The gene for Klebsiella bacteriophage K11 RNA polymerase: Sequence and comparison with the homologous genes of phages T7, T3, and SP6. Mol. Gen. Genet. 221, 283-286.
7. Dunn, J. J. and Studier, F. W. (1983) Complete nucleotide sequence of bacteriophage T7 DNA and locations of T7 genetic elements. J. Mol. Biol. 166, 477-535.
8. Han, K. G., Lee, S. S. and Kang, C. (1999) Soluble expression of cloned phage K11 RNA polymerase gene in Escherichia coli at a low temperature. Protein Express. Purif. 16, 103-108.
9. Han, K. G., Kim, D. H., Junn, E., Lee, S. S. and Kang, C. (2002) Identification of bacteriophage K11 genomic promoters for K11 RNA polymerase. J. Biochem. Mol. Biol. 35, 637-641.
10. Huang, J., Villemain, J., Padilla, R. and Sousa, R. (1999) Mechanisms by which T7 lysozyme specifically regulates T7 RNA polymerase during different phases of transcription. J. Mol. Biol. 293, 457-475.
11. Ikeda, R. A. and Bailey, P. A. (1992) Inhibition of T7 RNA polymerase by T7 lysozyme in vitro. J. Biol. Chem. 267, 20153-20158.
12. Inouye, M., Arnheim, N. and Sternglanz, R. (1973) Bacteriophage T7 lysozyme is an N-acetylmuramyl-L-alanine amidase. J. Biol. Chem. 25, 7247-7252.
13. Jeruzalmi, D. and Steitz, T. A. (1998) Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme. EMBO 17, 4101-4113.
14. Junn, H. J., Youn, J., Suh, K. H. and Lee, S. S. (2005) Cloning and expression of Klebsiella phage K11 lysozyme gene. Protein Express. Purif. 42, 78-84.
15. Moffatt, B. A. and Studier, F. W. (1987) T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell 49, 221-227.
16. Rosa, M. D. and Andrews, N. C. (1981) Phage T3 DNA contains an extra copy of 23 base-pair phage T7 RNA polymerase promoter sequence. J. Mol. Biol. 147, 41-53.
17. Singh, A. and Hayashi, K. (1995) Construction of chimeric β-glucosidases with improved enzymatic properties. J. Biol. Chem. 270, 21928-21933.
18. Terpe, K. (2003) Overview of tag protein fusions: from molecules and biochemical fundamentals to commercial systems. Appli. Microbiol. Biotechnol. 60, 523-533.
19. Zhang, X. and Studier, F. W. (1995) Isolation of transcriptionally active mutants of T7 RNA polymerase that do not support phage growth. J. Mol. Biol. 250, 156-168.
20. Zhang, X. and Studier, F. W. (2004) Multiple roles of T7 RNA polymerase and T7 lysozyme during bacteriophage T7 infection. J. Mol. Biol. 340, 707-730.