Studies of receptor tyrosine kinase transmembrane domain interactions: The EmEx-FRET method

Journal of Membrane Biology

Volumn 215, Issue 2-3, 2007, Pages 93-103

  Merzlyakov, Mikhail ^a   Chen, Lirong ^a   Hristova, Kalina ^a  

^a Johns Hopkins University   (United States)

Author keywords

FRET; Receptor tyrosine kinase; Transmembrane domain

Indexed keywords

ALANINE; ARGININE; ASPARTIC ACID; FIBROBLAST GROWTH FACTOR RECEPTOR 3; GLUTAMINE; GLYCINE; PROTEIN TYROSINE KINASE;

ACCURACY; ARTICLE; BILAYER MEMBRANE; CANCER CELL CULTURE; CONCENTRATION RESPONSE; DEREGULATION; DIMERIZATION; ENERGY TRANSFER; FLUORESCENCE RESONANCE ENERGY TRANSFER; GENE MUTATION; MEMBRANE STRUCTURE; MOLECULAR DYNAMICS; MULTIPLE MYELOMA; PATHOGENESIS; PROTEIN DOMAIN; PROTEIN INTERACTION; SIGNAL TRANSDUCTION; THERMODYNAMICS;

AMINO ACID SUBSTITUTION; BINDING SITES; DIMERIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; MUTATION; PROTEIN STRUCTURE, TERTIARY; RECEPTOR PROTEIN-TYROSINE KINASES; RECEPTOR, FIBROBLAST GROWTH FACTOR, TYPE 3;

EMEX;

EID: 34548013094     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-007-9009-0     Document Type: Article

References (27)

1. Adams PD, Arkin IT, Engelman DM, Brunger AT (1995) Computational searching and mutagenesis suggest a structure for the pentameric transmembrane domain of phospholamban. Nat Struct Biol 2(2):154-162
2. Blume-Jensen P, Hunter T (2001) Oncogenic kinase signalling. Nature 411:355-365
3. Cho JY, Guo CS, Torello M, Lunstrum GP, Iwata T, Deng CX, Horton WA (2004) Defective lysosomal targeting of activated fibroblast growth factor receptor 3 in achondroplasia. Proc Natl Acad Sci USA 101:609-614
4. Deng C, Wynshaw-Boris W, Zhou F, Kuo A, Leder P (1996) Fibroblast growth factor receptor 3 is a negative regulator of bone growth. Cell 84:911-921
5. Fantl WJ, Johnson DE, Williams LT (1993) Signaling by receptor tyrosine kinases. Annu Rev Biochem 62:453-481
6. Fisher LE, Engelman DM, Sturgis JN (1999) Detergents modulate dimerization, but not helicity, of the glycophorin A transmembrane domain. J Mol Biol 293:639-651
7. Han X, Mihailescu M, Hristova K (2006) Neutron diffraction studies of fluid bilayers with transmembrane proteins: structural consequences of the achondroplasia mutation. Biophys J 91(10):3736-3747
8. Iwamoto T, You M, Li E, Spangler J, Tomich JM, Hristova K (2005) Synthesis and initial characterization of FGFR3 transmembrane domain: Consequences of sequence modifications. Biochim Biophys Acta 1668:240-247
9. Lakowicz JR (1999) Principles of Fluorescence Spectroscopy. Kluwer Academic/Plenum Publishers, New York
10. Li E, Hristova K (2004) Imaging FRET measurements of transmembrane helix interactions in lipid bilayers on a solid support. Langmuir 20:9053-9060
11. Li E, Hristova K (2006) Role of receptor tyrosine kinase transmembrane domains in cell signaling and human pathologies. Biochemistry 45:6241-6251
12. Li E, You M, Hristova K (2005) SDS-PAGE and FRET suggest weak interactions between FGFR3 TM domains in the absence of extracellular domains and ligands. Biochemistry 44:352-360
13. Li E, You M, Hristova K (2006) FGFR3 dimer stabilization due to a single amino acid pathogenic mutation. J Mol Biol 356:600-612
14. Magde D, Wong R, Seybold PG (2002) Fluorescence quantum yields and their relation to lifetimes of rhodamine 6G and fluorescein in nine solvents: Improved absolute standards for quantum yields. Photochem Photobiol 75:327-334
15. Merzlyakov M, You M, Li E, Hristova K (2006) Transmembrane helix heterodimerization in lipids bilayers: Probing the energetics behind autosomal dominant growth disorders. J Mol Biol 358:1-7
16. Meyers GA, Orlow SJ, Munro IR, Przylepa KA, Jabs EW (1995) Fibroblast-growth-factor-receptor-3 (Fgfr3) transmembrane mutation in Crouzon syndrome with Acanthosis nigricans. Nat Genet 11:462-464
17. Otsuki T, Nakazawa N, Taniwaki M, Yamada O, Sakaguchi H, Wada H, Yawata Y, Ueki A (1998) Establishment of a new human myeloma cell line, KMS-18, having t(4;14)(p16.3;q32.3) derived from a case phenotypically transformed from Ig A-lambda to BJP-lambda, and associated with hyperammonemia. Int J Oncol 12:545-552
18. Raicu V, Jansma DB, Miller RJD, Friesen JD (2005) Protein interaction quantified in vivo by spectrally resolved fluorescence resonance energy transfer. Biochem J 385:265-277
19. Ronchetti D, Greco A, Compasso S, Colombo G, Dell'Era P, Otsuki T, Lombardi L, Neri A (2001) Deregulated FGFR3 mutants in multiple myeloma cell lines with t(4;14): Comparative analysis of Y373C, K650E and the novel G384D mutations. Oncogene 20:3553-3562
20. Schlessinger J (2000) Cell signaling by receptor tyrosine kinases. Cell 103:211-225
21. Shiang R, Thompson LM, Zhu Y-Z, Church DM, Fielder TJ, Bocian M, Winokur ST, Wasmuth JJ (1994) Mutations in the transmembrane domain of FGFR3 cause the most common genetic form of dwarfism, achondroplasia. Cell 78:335-342
22. Vajo Z, Francomano CA, Wilkin DJ (2000) The molecular and genetic basis of fibroblast growth factor receptor 3 disorders: The achondroplasia family of skeletal dysplasias, Muenke craniosynostosis, and Crouzon syndrome with acanthosis nigricans. Endocr Rev 21:23-39
23. van der Geer P, Hunter T, Lindberg RA (1994) Receptor protein-tyrosine kinases and their signal transduction pathways. Annu Rev Cell Biol 10:251-337
24. van Rhijin B, van Tilborg A, Lurkin I, Bonaventure J, de Vries A, Thiery JP, van der Kwast TH, Zwarthoff E, Radvanyi F (2002) Novel fibroblast growth factor receptor 3 (FGFR3) mutations in bladder cancer previously identified in non-lethal skeletal disorders. Eur J Hum Genet 10:819-824
25. Webster MK, Donoghue DJ (1996) Constitutive activation of fibroblast growth factor receptor 3 by the transmembrane domain point mutation found in achondroplasia. EMBO J. 15:520-527
26. You M, Li E, Hristova K (2006) The achondroplasia mutation does not alter the dimerization energetics of FGFR3 transmembrane domain. Biochemistry 45:5551-5556
27. You M, Li E, Wimley WC, Hristova K (2005) FRET in liposomes: Measurements of TM helix dimerization in the native bilayer environment. Anal Biochem 340:154-164