A combination of neutral loss and targeted product ion scanning with two enzymatic digestions facilitates the comprehensive mapping of phosphorylation sites

Proteomics

Volumn 7, Issue 15, 2007, Pages 2522-2529

  Casado Vela, Juan ^a   Ruiz, Edgar J ^a   Nebreda, Angel R ^a   Casal, J Ignacio ^a  

^a Centro Nacional de Investigaciones Oncológicas   (Spain)

Author keywords

Cdc25; Linear ion trap; Neutral loss; Phosphorylation analysis; TPIS

Indexed keywords

CHYMOTRYPSIN; CYCLIN B; CYCLIN DEPENDENT KINASE 1; PROTEIN TYROSINE PHOSPHATASE; PROTEINASE; SERINE; THREONINE; TRYPSIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CELL CYCLE; CONTROLLED STUDY; ENZYME ACTIVITY; EUKARYOTIC CELL; FRAGMENTATION REACTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; XENOPUS LAEVIS;

AMINO ACID SEQUENCE; ANIMALS; CDC25 PHOSPHATASE; CELL CYCLE PROTEINS; CHYMOTRYPSIN; ESCHERICHIA COLI; GLUTATHIONE TRANSFERASE; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; TRYPSIN; XENOPUS;

EUKARYOTA; XENOPUS LAEVIS;

EID: 34547916086     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.200700026     Document Type: Article

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