Co-transformation of gene expression cassettes via particle bombardment to generate safe transgenic plant without any unwanted DNA

In Vitro Cellular and Developmental Biology - Plant

Volumn 43, Issue 4, 2007, Pages 328-334

  Zhao, Yan ^a   Qian, Qian ^b   Wang, Hui Zhong ^c   Huang, Da Nian ^b  

^a ZHEJIANG GONGSHANG UNIVERSITY   (China)

^b CHINA NATIONAL RICE RESEARCH INSTITUTE   (China)

^c HANGZHOU NORMAL UNIVERSITY   (China)

Author keywords

Hereditary segregation strategy; Minimal gene cassettes; Rice (Oryza sativa L.); Selectable marker free

Indexed keywords

ORYZA SATIVA;

EID: 34547841304     PISSN: 10545476     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11627-007-9051-8     Document Type: Article

References (32)

1. Afolabi, A. S.; Worland, B.; Snape, J. W.; Vain, P. A large-scale study of rice plants transformed with different T-DNAs provides new insights into locus composition and T-DNA linkage configurations. Theor. Appl. Genet. 4:815-826; 2004
2. Altpeter, F.; Baisakh, N.; Beachy, R.; Bock, R.; Capell, T.; Christou, P.; Daniell, H.; Datta, K.; Datta, S.; J. Dix, P.; Fauquet, C.; Huang, N.; Kohli, A.; Mooibroek, H.; Nicholson, L.; N guyen, T. T.; Nugent, G.; Raemakers, K.; Romano, A.; A. Somers, D.; Stoger, E.; Taylor, N.; Visser, R. Particle bombardment and the genetic enhancement of crops: myths and realities. Mol. Breed. 15:305-327; 2005
3. Breitler, J. C.; Labeyrie, A.; Meynard, D.; Legavre, T.; Guiderdoni, E. Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes. Theor. Appl. Genet. 104:709-719; 2002
4. Cao, M. X.; Huang, J. Q.; Yao, Q. H.; Liu, S. J.; Wang, C. L.; Wei, Z. M. Site-secific DNA excision in transgenic rice with a cell-permeable cre recombinase. Mol. Biotechnol. 1:55-63; 2006
5. Edwards, K.; Johnston, C.; Thompson, C. A simple and rapid method for the preparation of plant genomic DNA for PCR analyses. Nucleic Acids Res. 98:1349; 1991
6. Fu, X. D.; Duc, L. T.; Fontana, S.; Bong, B. B.; Tinjuangjun, P.; Sudhakar, D.; Twyman, R. M.; Christou P.; Kohli, A. Linear transgene constructs lacking vector backbone sequences generate low-copy-number transgenic plants with simple integration patterns. Transgenic Res. 9:11-19; 2000
7. Gleave, A. P.; Mitra, D. S.; Mudge, S. R.; Morris B. A. M. Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene. Plant Mol. Biol. 40:223-235; 1999
8. Holger, P. Removing selectable marker genes: taking shortcut. Trends in Plant Sci. 7:273-274; 2000
9. Holger, P. Towards the ideal GMP: homologous recombination and marker gene excision. J. Plant Physiol. 7:743-754; 2003
10. Hightower, R.; Baden, C.; Penzes, E.; Dunsmuir, P. The expression of cecropin peptide in transgenic tobacco does not confer resistance to Psuedomanas syringae pv tabaci. Plant Cell Reports. 13:295-299; 1994
11. Huang, D. N.; Zhu, B.; Yang, W.; Xue, R.; Xiao, H.; Tian, W. Z.; Li, L. C.; Dai, S. H. Introduction of cecropinB gene into rice (Oryza sativa L) by particle gun bombardment and analysis of transgenic plants. Sci. China (Ser. C). 39:652-661; 1996
12. Huang, S.; Gilbertson, L. A.; Adams, T. H.; Malloy, K. P.; Reisenbigler, E. K.; Birr, D. H., Snyder, M. W.; Zhang, Q.; Luethy, M. H. Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors. Transgenic Res. 5:451-461; 2004
13. Jia H.; Pang, Y; Chen, X.; Fang R. Removal of the selectable marker gene from transgenic tobacco plants by expression of Cre recombinase from a tobacco mosaic virus vector through agroinfecion. Transgenic Res. 3:375-384; 2006
14. Kohli, A.; Griffiths, S.; Palacios, N.; Twyman, R. M.; Vain, P.; Laurie, D. A.; Christou, P. Molecular characterization of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV 35S promoter and confirms the predominance of microhomology mediated recombination. Plant J. 6:591-601; 1999
15. Komari T, Hiei Y, Satio Y, Murai N, Kumashiro T. Vestors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J. 1:165-174; 1996
16. Lange, M.; Vincze, E.; Moller, M. G.; Holm, P. B. Molecular analysis of transgene and vector backbone integration into the barley genome following Agrobacterium-mediated transformation. Plant Cell Rep. 8:815-820; 2006
17. Loc, N. T.; Tinjuangjun, P.; Gatehouse, A. M. R.; Christou, P.; Gatehouse, J. A. Linear transgene constructs lacking vector backbone sequences generate transgenic rice plants which accumulate higher levels of proteins conferring insect resistance. Mol. Breed. 9:231-244; 2002
18. Lu, Y. J.; Zheng K. L. A simple method for isolation of rice DNA. Chinese J Rice Sci. 1992, 1: 47-48 (article in Chinese)
19. Lutz, K. A.; Bosacchi, M. H.; Maliga, P. Plastid marker-gene excision by transiently expressed CRE recombinase. Plant J. 3:447-456; 2006
20. Matthews, P. R.; Wang, M. B.; Waterhouse, P. M.; Thornton, S.; Fieg, S. J.; Gubler, F.; Jacobsen, J. V. Marker gene elimination from transgenic barley, using co-transformation with adjacent 'twin T-DNAs' on a standard Agrobacterium transformation vector. Mol. Breed. 7:195-202; 2001
21. Miki, B.; McHugh, S. Selectable marker genes in transgenic plants: applications, alternative and biosafety. J. Biotechnol. 107:193-232; 2004
22. Miller, M.; Tagliani, L.; Wang, N.; Berka, B.; Bidney, D.; Zhao, Z. Y. High efficiency transgene segregation in co-transformed maize plants using an Agrobacterium tumefaciens 2 T-DNA binary system. Transgenic Res. 11:381-396; 2002
23. Park, J.; Lee, Y. K.; Kang, B. K.; Chung, W. I. Co-transformation using a negative selectable gene for the production of selectable marker gene-free transgenic plants. Theor. Appl. Genet. 109:1562-1567; 2004
24. Romano, A.; Raemakers, K.; Bernardi, J.; Visser, R.; Mooibroek, H. Transgene organization in potato after particle bombardment-mediated (co) transformation using plasmids and gene cassettes. Transgenic Res. 12:461-473; 2003
25. Sambrook, J.; Fritsch, E. F.; Maniantis, T. Molecular cloning: a laboratory Manual. 2nd edn. New York: Cold Spring Harbor Laboratory Press; 1989
26. Sugtia, K.; Kasahara, T.; Matsunage, E.; Ebinuma. A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J. 5:461-469; 2000
27. Vidal, J. R.; Kikkert, J. R.; Donzelli, B. D.; Wallace, P. G.; Reisch, B. I. Biolistic transformation of grapevine using minimal gene cassette technology. Plant Cell Rep. 8:807-814; 2006
28. Xia, Z. H.; Li, X. B.; Chen, C.Y.; Fan, H. K.; Jiang, G. H.; Zhu, L. H.; Zhai, W. X. Generation of selectable marker-free and vector backbone sequence-free Xa21 transgenic rice. Chin J Biotechnol. 2:204-210; 2006 (article in Chinese)
29. Yao, Q.; Cong, L.; Wang; Y. S.; Chen, M. J.; Yang, G. X. Inheritance of the foreign gene cassettes lacking vector backbone sequences. HEREDITAS (Beijing). 6:695-698; 2006 (article in Chinese)
30. Zhang, W.; Subbarao, S.; Addae, P.; Shen, C.; Armstrong, C.; Peschke, V.; Gilbertson, L. Cre/lox-mediated marker gene excision in transgenic maiza (Zea mays L.) plants. Theor. Appl. Genet. 107:1157-1168; 2003
31. Zhao, Y.; Yu Y.C.; Qian Q; Yan M. X.; Huang D. N. Cotransformation of rice by bar and cecropin B gene expression cassettes lacking vector backbone sequences. Acta Genet Sin. 2:135-141; 2003 (article in Chinese).
32. Zubko, E.; Scutt, C.; Meyer, P. Intrachromosomal recombination between attP regions as a tool to remove selectable marker genes from tobacco transgenes. Nat Biotechnol. 18:442-445; 2000