Site-specific DNA excision in transgenic rice with a cell-permeable Cre recombinase

Molecular Biotechnology

Volumn 32, Issue 1, 2006, Pages 55-63

  Cao, Ming Xia ^a   Huang, Jian Qiu ^a   Yao, Quan Hong ^b   Liu, Sheng Jun ^a   Wang, Cheng Long ^a   Wei, Zhi Ming ^a  

^a Shanghai Jiao Tong University Affiliated Sixth People s Hospital   (China)

^b INSTITUTE OF EDIBLE FUNGI   (China)

Author keywords

Biosafety; Cell permeable Cre recombinase; Marker free; MTS; Transgenic rice plants

Indexed keywords

AMINO ACIDS; BIOLOGICAL MEMBRANES; CELLS; ENZYMES; GENETIC ENGINEERING; PLANT CELL CULTURE; PLANTS (BOTANY);

BIOSAFETY; CELL-PERMEABLE CRE RECOMBINASE; MARKER-FREE; MTS; TRANSGENIC RICE PLANTS;

DNA;

AMINO ACID; CRE RECOMBINASE; DNA; FIBROBLAST GROWTH FACTOR 4;

AMINO ACID SEQUENCE; ARTICLE; CELL MEMBRANE PERMEABILITY; CULTIVAR; DNA RECOMBINATION; GENE TRANSLOCATION; MARKER GENE; NONHUMAN; PLANT CELL; PROTEIN EXPRESSION; PROTEIN TRANSPORT; RICE; TRANSGENIC PLANT;

EID: 29644442933     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.apenergy.2005.01.005     Document Type: Article

References (22)

1. Hohn, B., Levy, A. A., and Puchta, H. (2001) Elimination of selection markers from transgenic plants. Curr. Opin. Biotechnol. 12, 139-143.
2. Miki, B. and McHugh, S. (2004) Selectable marker genes in transgenic plants: applications, alternatives and biosafety. J. Biotechnol. 107, 193-232.
3. Matzke, M. A. Mette, M. F., and Matzke, A. J. M. (2000) Transgene silencing by the host genome defense: implications for tyhe evolution of epigenetic control mechanisms in plants and vertebrates. Plant Mol. Biol. 43, 401-415 (special issue on Plant Gene Silencing).
4. Goldsbrough, A. P., Lastrella, C. N., and Yoder J. I. (1993) Transposition mediated re-positioning and subsequent elimination of marker genes from transgenic plants. Bio/Technology 11, 1286-1292.
5. Luo H. and Kausch A. P. (2002) Application of FLP/FRT site-specific DNA recombination system in plants. Genet. Eng. (NY) 24, 1-16.
6. Radhakrishnan P. and Srivastava V. (2005) Utility of the FLP-FRT recombination system for genetic manipulation of rice. Plant Cell Rep. 23, 721-726.
7. Sugita, K., Kasahara, T., Matsunaga, E., and Ebinuma, H. (2000) A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency. Plant J. 5, 461-469.
8. Ebinuma, H., Sugita, K., Endo, S., Matsunaga, E., and Yamada, K. (2005) Elimination of marker genes from transgenic plants using MAT vector systems. Methods Mol. Biol. 286, 237-254.
9. Dale, E. C., and Ow, D. W. (1991) Gene transfer with subsequent removal of the selection gene from the host genome. Proc. Natl. Acad. Sci. USA 88, 10,558-10,562.
10. Russell, S. H., Hoopes, J. L., and Odell, J. T. (1992) Directed excision of a transgene from the plant genome. Mol. Gen. Genet. 234, 49-59.
11. Zuo, J., Niu, Q. W., Moller, S. G., and Chua, N. H. (2001) Chemical-regulated, site-specific DNA excision in transgenic plants. Nat. Biotechnol. 19, 157-161.
12. Gleave, A. P., Mitra, D. S., Mudge, S. R., and Morris, B. A. (1999) Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene. Plant Mol. Biol. 40, 223-235.
13. Sauer, B. (1987) Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae. Mol. Cell Biol. 7, 2087-2096.
14. Hoff, T., Schnorr, K. M., and Mundy, J. (2001) A recombinase-mediated transcriptional induction system in transgenic plants. Plant Mol. Biol. 45, 41-49.
15. Jo, D., Nashabi, A., Doxsee, C., et al. (2001) Epigenetic regulation of gene structure and function with a cell-permeable Cre recombinase. Nature Biotechnol. 19, 929-933.
16. Torgerson, T. R., Colosia, A. D., Donahue, J. P., Lin, Y. Z., and Hawiger, J. (1998) Regulation of NF-kappa B, AP-1, NFAT, and STAT1 nuclear import in T lymphocytes by noninvasive delivery of peptide carrying the nuclear localization sequence of NF-kappa B p50. J. Immunol. 161, 6084-6092.
17. Rojas, M., Donahue, J. P., Tan, Z., and Lin, Y. Z. (1998) Genetic engineering of proteins with cell membrane permeability. Nat. Biotechnol. 16, 370-375.
18. Cao, M. X., Huang, J. Q., Wei, Z. M., Yao, Q. H., Wan, C. Z., and Lu, J. A. (2004) Engineering higher yield and herbicide resistance in rice by Agrobacterium-mediated multiple gene transformation. Crop Sci. 44, 2206-2213.
19. Chu, C. C., Wang, C. S., Sun, C. S., et al. (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. 18, 659-668.
20. Jefferson, R.A. (1987) Assay chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5, 387-405.
21. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tabacco tissue cultures. Physiol. Plant. 15, 473-497.
22. Murray, M. G. and Thompson, W. F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res. 8, 4321-4325.