PC1/3, PC2 and PC5/6A are targeted to dense core secretory granules by a common mechanism

FEBS Journal

Volumn 274, Issue 16, 2007, Pages 4094-4102

  Dikeakos, Jimmy D ^a   Mercure, Chantal ^a   Lacombe, Marie Josée ^a   Seidah, Nabil G ^a   Reudelhuber, Timothy L ^a  

^a CLIN RESEARCH INSTITUTE OF MONTREAL   (Canada)

Author keywords

Alpha helix; PC5 6; Proprotein convertases; Regulated secretion; Secretory granules

Indexed keywords

HYBRID PROTEIN; PROPROTEIN CONVERTASE 1; PROPROTEIN CONVERTASE 2; PROPROTEIN CONVERTASE 5;

ALPHA HELIX; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; ENDOCRINE CELL; HYDROPHOBICITY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN SECRETION; PROTEIN STRUCTURE; PROTEIN TARGETING; SECRETORY GRANULE;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CELL LINE; CYTOPLASM; HYDRAZINES; MICE; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; PROPROTEIN CONVERTASE 1; PROPROTEIN CONVERTASE 2; PROPROTEIN CONVERTASE 5; PROTEIN STRUCTURE, SECONDARY; PROTEIN TRANSPORT; SECRETORY VESICLES; TRANSFECTION;

EID: 34547759249     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2007.05937.x     Document Type: Article

References (24)

1. Seidah NG Prat A (2002) Precursor convertases in the secretory pathway, cytosol and extracellular milieu. Essays Biochem 38, 79 94.
2. Dikeakos JD Reudelhuber TL (2007) Sending proteins to dense core secretory granules: still a lot to sort out. J Cell Biol 177, 191 196.
3. Jutras I, Seidah NG Reudelhuber TL (2000) A predicted alpha-helix mediates targeting of the proprotein convertase PC1 to the regulated secretory pathway. J Biol Chem 275, 40337 40343.
4. Zhou A, Paquet L Mains RE (1995) Structural elements that direct specific processing of different mammalian subtilisin-like prohormone convertases. J Biol Chem 270, 21509 21516.
5. Creemers JW, Usac EF, Bright NA, Van de Loo JW, Van d Jansen E Hutton JC (1996) Identification of a transferable sorting domain for the regulated pathway in the prohormone convertase PC2. J Biol Chem 271, 25284 25291.
6. Assadi M, Sharpe JC, Snell C Loh YP (2004) The C-terminus of prohormone convertase 2 is sufficient and necessary for Raft association and sorting to the regulated secretory pathway. Biochemistry 43, 7798 7807.
7. Barbero P, De Rovere CBI, Seidah N, Beaudet A Kitabgi P (1998) PC5-A-mediated processing of pro-neurotensin in early compartments of the regulated secretory pathway of PC5-transfected PC12 cells. J Biol Chem 273, 25339 25346.
8. De Bie I, Marcinkiewicz M, Malide D, Lazure C, Nakayama K, Bendayan M Seidah NG (1996) The isoforms of proprotein convertase PC5 are sorted to different subcellular compartments. J Cell Biol 135, 1261 1275.
9. Schmidt WK Moore HP (1995) Ionic milieu controls the compartment-specific activation of pro-opiomelanocortin processing in AtT-20 cells. Mol Biol Cell 6, 1271 1285.
10. Cuff JA, Clamp ME, Siddiqui AS, Finlay M Barton GJ (1998) JPred: a consensus secondary structure prediction server. Bioinformatics 14, 892 893.
11. Rost B, Yachdav G Liu J (2004) The PredictProtein server. Nucleic Acids Res 32, W321 W326.
12. Mouchantaf R, Kumar U, Sulea T Patel YC (2001) A conserved alpha-helix at the amino terminus of prosomatostatin serves as a sorting signal for the regulated secretory pathway. J Biol Chem 276, 26308 26316.
13. Dhanvantari S, Arnaoutova I, Snell CR, Steinbach PJ, Hammond K, Caputo GA, London E Loh YP (2002) Carboxypeptidase E, a prohormone sorting receptor, is anchored to secretory granules via a C-terminal transmembrane insertion. Biochemistry 41, 52 60.
14. Garcia AL, Han SK, Janssen WG, Khaing ZZ, Ito T, Glucksman MJ, Benson DL Salton SR (2005) A prohormone convertase cleavage site within a predicted alpha-helix mediates sorting of the neuronal and endocrine polypeptide VGF into the regulated secretory pathway. J Biol Chem 280, 41595 41608.
15. Dikeakos JD, Lacombe MJ, Mercure C, Mireuta M Reudelhuber TL (2007) A hydrophobic patch in a charged alpha-helix is sufficient to target proteins to dense core secretory granules. J Biol Chem 282, 1136 1143.
16. Blazquez M, Docherty K Shennan KI (2001) Association of prohormone convertase 3 with membrane lipid rafts. J Mol Endocrinol 27, 107 116.
17. Blazquez M, Thiele C, Huttner WB, Docherty K Shennan KI (2000) Involvement of the membrane lipid bilayer in sorting prohormone convertase 2 into the regulated secretory pathway. Biochem J 349 (Part 3 843 852.
18. Arnaoutova I, Smith AM, Coates LC, Sharpe JC, Dhanvantari S, Snell CR, Birch NP Loh YP (2003) The prohormone processing enzyme PC3 is a lipid raft-associated transmembrane protein. Biochemistry 42, 10445 10455.
19. Wegrzyn J, Lee J, Neveu JM, Lane WS Hook V (2007) Proteomics of neuroendocrine secretory vesicles reveal distinct functional systems for biosynthesis and exocytosis of peptide hormones and neurotransmitters. J Proteome Res 6, 1652 1665.
20. Lee MC, Orci L, Hamamoto S, Futai E, Ravazzola M Schekman R (2005) Sar1p N-terminal helix initiates membrane curvature and completes the fission of a COPII vesicle. Cell 122, 605 617.
21. Brechler V, Chu WN, Baxter JD, Thibault G Reudelhuber TL (1996) A protease processing site is essential for prorenin sorting to the regulated secretory pathway. J Biol Chem 271, 20636 20640.
22. Benjannet S, Reudelhuber T, Mercure C, Rondeau N, Chretien M Seidah NG (1992) Proprotein conversion is determined by a multiplicity of factors including convertase processing, substrate specificity, and intracellular environment. Cell type-specific processing of human prorenin by the convertase PC1. J Biol Chem 267, 11417 11423.
23. Rice P, Longden I Bleasby A (2000) EMBOSS: the European Molecular Biology Open Software Suite. Trends Genet 16, 276 277.
24. Cuff JA Barton GJ (2000) Application of multiple sequence alignment profiles to improve protein secondary structure prediction. Proteins 40, 502 511.