Detection of pathogenic leptospires in animals by PCR based on lipL21 and lipL32 genes

Indian Journal of Experimental Biology

Volumn 45, Issue 6, 2007, Pages 568-573

  Cheema, P S ^a   Srivastava, S K ^a   Amutha, R ^a   Singh, S ^a   Singh, H ^a   Sandey, M ^a  

^a INDIAN VETERINARY RESEARCH INSTITUTE   (India)

Author keywords

Leptospira; Outer membrane proteins; Polymerase chain reaction

Indexed keywords

ANIMALIA; BOS; BRUCELLA; BUBALUS; CAMPYLOBACTER; CAVIA; LEPTOSPIRA; PASTEURELLA;

BACTERIAL DNA; LIPL32 PROTEIN, LEPTOSPIRA; LIPOPROTEIN; OUTER MEMBRANE PROTEIN; UNCLASSIFIED DRUG;

ANIMAL; ARTICLE; BUFFALO; EVALUATION; GENETICS; GUINEA PIG; ISOLATION AND PURIFICATION; LEPTOSPIRA; LEPTOSPIROSIS; METHODOLOGY; MICROBIOLOGY; PATHOGENICITY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

ANIMALS; BACTERIAL OUTER MEMBRANE PROTEINS; BUFFALOES; DNA, BACTERIAL; GUINEA PIGS; LEPTOSPIRA; LEPTOSPIROSIS; LIPOPROTEINS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 34547751435     PISSN: 00195189     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Haake D A, Martinich A, Summers T A, Shang E S, Pruetz J D, McCoy A M, Mazel M K & Bolin C A, Characterization of leptospiral outer membrane lipoprotein LipL36: Downregulation associated with late log-phase growth and mammalian infection, Infect Immun, 66 (1998) 1579.
2. Faine S, Guidelines for the control of leptospirosis, Offset Publication No 67, (World Health Organization, Geneva), 1982.
3. Eaglesome M D & Garcia M M, Microbial agents associated with bovine genital tract infection and semen, Part I - Brucella abortus, Leptospira, Campylobacter fetus and Trichomonas foetus, Vet Bull, 62 (1992) 743
4. Van Eys G J J M, Zaal J, Schoone G J & Terpstra W J, DNA hybridization with Hardjo bovis-specific recombinant probes as a method for Type discrimination of Leptospira interrogans Serovar hardjo, J Gen Microbiol, 134 (1988) 567.
5. Gravekamp C, Van der Kemp H, Franzen M, Carrington D & Schoone G J, Detection of seven species of pathogenic leptospires by PCR using two sets of primer, J Gen Microbiol, 139 (1993) 1691.
6. Heinemann M B, Garcia J F, Nunes C M, Morais Z M, Gregori F, Cortez A, Vasconcellos S A, Visintin J A & Richtzenhain L J, Detection of leptospires in bovine semen by polymerase chain reaction, Aust Vet J, 77 (1999) 32.
7. Heinemann M B, Garcia J F, Nunes C M, Gregori F, Higa Z M M, Vasconcellos S A & Richtzenhain L J, Detection and differentiation of Leptospira spp. Serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism, Vet Microbiol, 73 (2000) 261.
8. Senthil Kumar A, Ramadass P & Nachimuthu K, Use of polymerase chain reaction for the detection of the leptospires in clinical samples, Indian Vet J, 78 (2001) 1087.
9. Harkin K R, Roshto Y M & Sullvan J T, Clinical application of polymerase reaction assay for diagnosis of leptospirosis in dogs, J Am Vet Med Assoc, 222 (2003) 1224.
10. Oliveira M A, Cabellero O L, Vago A R, Hartskeerl R A, Romnah A J, Pena S D, Simpson A J & Kaury M C, Lowstringency single specific primer PCR for identification of Leptospira, J Med Microbiol, 52 (2003) 127.
11. Haake D A, Chao G, Zuerner R L, Barnett J K, Barnett D, Mazel M, Matsunaga J, Levett P N & Bolin C A, The leptospiral major outer membrane protein LipL32 is a lipoprotein expressed during mammalian infection, Infect Immun, 68 (2000) 2276.
12. Cullen P A, Haake D A, Bulach D M, Zuerner R L & Adler B, LipL21 is a novel surface exposed lipoprotein of pathogenic Leptospira species, Infect Immun, 71 (2003) 2414.
13. Guerreiro H, Croda J, Flannery B, Mazel M, Matsunaga J, Reis M G, Levett P N, Ko A I & Haake D A, Leptospiral proteins recognized during the humoral immune response to leptospirosis in humans, Infect Immun, 69 (2001) 4958.
14. Johnson R C, Walby J, Henry R A & Auran N E, Cultivation of parasitic leptospires: Effect of pyruvate, Appl Microbiol, 26 (1973) 118.
15. Ausubel F M, Brent R, Kingston R E, Moore D D, Seidman J G, Smith J A & Struhl K, Current Protocols in Molecular Biology, in Current protocols Vol I (John Wiley and Sons, Inc, USA) 1995, 2.4.1.
16. Merien F, Amouriaux P, Perolat P, Baranton G & Saint Girons I, Polymerase chain reaction for detection of Leptospira spp in clinical samples, J Clin Micro, 30 (1992) 2219.