Detection and differentiation of Leptospira spp. serovars in bovine semen by polymerase chain reaction and restriction fragment length polymorphism

Veterinary Microbiology

Volumn 73, Issue 4, 2000, Pages 261-267

  Heinemann, Marcos Bryan ^a   Garcia, José Fernando ^b   Nunes, Cáris Maroni ^b   Gregori, Fabio ^a   Higa, Zenaide Maria Moraes ^a   Vasconcellos, Silvio Arruda ^a   Richtzenhain, Leonardo José ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b S Paulo State University   (Brazil)

Author keywords

Cattle bacteria; Leptospira spp.; PCR; RFLP

Indexed keywords

RESTRICTION ENDONUCLEASE;

ARTICLE; BACTERIUM IDENTIFICATION; CATTLE; CATTLE DISEASE; LEPTOSPIRA; LEPTOSPIRA INTERROGANS; LEPTOSPIROSIS; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SPERM;

ANIMALS; CATTLE; CATTLE DISEASES; DNA PRIMERS; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; INSEMINATION, ARTIFICIAL; LEPTOSPIRA; LEPTOSPIRA INTERROGANS; LEPTOSPIROSIS; MALE; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEMEN;

BACTERIA (MICROORGANISMS); BOS TAURUS; BOVINAE; LEPTOSPIRA; LEPTOSPIRA BIFLEXA; LEPTOSPIRA BORGPETERSENII; LEPTOSPIRA INTERROGANS; LEPTOSPIRA NOGUCHII; LEPTOSPIRA SANTAROSAI;

EID: 0034636397     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1135(00)00150-4     Document Type: Article

References (15)

1. Brod C.S., Martins L.F.S., Nussbaum J.R., Fehlberg M.F.B., Furtado L.R.I., Rosado R.L.I. Leptospirose bovina na região Sul do Estado do Rio Grande do Sul. A Hora Veterinária. 14:1994;15-20.
2. Eaglesome M.D., Garcia M.M. Microbial agents associated with bovine genital tract infections and semen. Part 1. Brucella abortus, Leptospira, Campylobacter fetus and Tritrichomonas foetus. Vet. Bull. 62:1992;743-775.
3. Ellis W.A., Cassels J.A., Doyle J. Genital leptospirosis in the bull. Vet. Rec. 118:1986;333.
4. Faine S. Guidelines for leptospirosis control. WHO, Geneva. 67:1982;171.
5. Gerritsen M.J., Olyhoek T., Smits M.A., Bokhout B.A. Sample preparation method for polymerase chain reaction-based semiquantitative detection of Leptospira interrogans serovar hardjo subtype hardjobovis in bovine urine. J. Clin. Microbiol. 29:1991;2805-2808.
6. Giorgi W., Teruya J.M., Silva Genovez M.E. Leptospirose: resultados das soroaglutinações realizadas no Instituto Biológico de São Paulo, durante os anos de 1974/1980. Biológico. 47:1981;299-309.
7. Mérien F., Amouriaux P., Perolat P., Baranton G., Saint Giron I. Polymerase chain reaction for detection of Leptospira spp. in clinical samples. J. Clin. Microbiol. 30:1992;2219-2224.
8. Moreira E.C., Silva J.A., Viana F.C., Santos W.L.M., Anselmo F.P., Leite R.C. Leptospirose bovina I. Aglutininas anti-leptospiras em soros sanguíneos de bovinos de Minas Gerais. Arq. Esc. Vet. Univers. Fed. Minas Gerais. 31:1979;375-388.
9. Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual. New York, Cold Spring Harbor Press, 1989, 956 p.
10. Santos F.R., Pena S.D.J., Epplen J.T. Genetic and population study of a Y-linked tetranucleotide repeat DNA polymorphism with a simple non-isotopic technique. Hum. Gen. 90:1993;655-666.
11. Savio M.L., Rossi C., Fusi P., Tagliabue S., Pacciarini M.L. Detection and identification of Leptospira interrogans serovars by PCR coupled with restriction endonuclease analysis of amplified DNA. J. Clin. Microbiol. 32:1994;935-941.
12. Schönberg A. Studies on the effect of antibiotic substances on leptospires and their cultivation from material with a high bacterial count. Zent. Baketeriol. Parasit. Infektion. Hyg. 249:1981;400-406.
13. Turner L.H. Leptospirosis III. Maintenance, isolation and demonstration of leptospires. Trans. Royal Soc. Trop. Med. Hyg. 64:1970;623-646.
14. Van Eys G.J., Gravekamp C., Gerritsen M.J., Quint W., Cornelissen M.P.E., Schegget J.T., Terpstra W.J. Detection of leptospires in urine by polymerase chain reaction. J. Clin. Microbiol. 27:1989;2258-2262.
15. Woodward M.J., Redstone J.S. Differentiation of Leptospira serovars by polymerase chain reaction and restriction fragment length polymorphism. Vet. Rec. 132:1993;325-326.