Horseradish peroxidase cDNA as a marker for electron microscopy in neurons

Journal of Neuroscience Methods

Volumn 165, Issue 2, 2007, Pages 210-215

  Schikorski, Thomas ^a   Young Jr , Samuel M ^b   Hu, Yunming ^a  

^a UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

^b MAX PLANCK INSTITUTE FOR BIOPHYSICAL CHEMISTRY   (Germany)

Author keywords

Electron microscopy; Endoplasmic reticulum; Gene transfer; Hippocampal neurons; Horseradish peroxidase

Indexed keywords

BIOLOGICAL MARKER; COMPLEMENTARY DNA; DIAMINOBENZIDINE; GLUTARALDEHYDE; HORSERADISH PEROXIDASE;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; CYTOLOGY; DENDRITE; ELECTRON MICROSCOPY; ENDOPLASMIC RETICULUM; GENE TRANSFER; GENETIC TRANSFECTION; MOLECULAR GENETICS; NERVE CELL; NEWBORN; NONHUMAN; PRIORITY JOURNAL; RAT; SYNAPSE;

3,3'-DIAMINOBENZIDINE; ANIMALS; ANIMALS, NEWBORN; BIOLOGICAL MARKERS; CELLS, CULTURED; DNA, COMPLEMENTARY; ENDOPLASMIC RETICULUM; GENES, REPORTER; HORSERADISH PEROXIDASE; HUMANS; MICROSCOPY, ELECTRON, TRANSMISSION; MOLECULAR BIOLOGY; NEURONS; PROMOTER REGIONS (GENETICS); RATS; STAINING AND LABELING; SYNAPSINS; TRANSFECTION;

EID: 34547657630     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jneumeth.2007.06.004     Document Type: Article

References (16)

1. Boyer C., Schikorski T., and Stevens C.F. Comparison of hippocampal dendritic spines in culture and in brain. J Neurosci 18 (1998) 5294-5300
2. Chalfie M., Tu Y., Euskirchen G., Ward W.W., and Prasher D.C. Green fluorescent protein as a marker for gene expression. Science 263 (1994) 802-804
3. Connolly C.N., Futter C.E., Gibson A., Hopkins C.R., and Cutler D.F. Transport in and out of the Golgi complex studied by transfecting cells with cDNAs encoding horseradish peroxidase. J Cell Biol 127 (1994) 641-652
4. Gaietta G., Deernick T.J., Adams S.R., Bouwer J., Tour O., Laird D.W., et al. Multicolor and electron microscopic imaging of connexin trafficking. Science 296 (2002) 503-507
5. Griffin B.A., Adams S.R., and Tsien R.Y. Specific covalent labeling of recombinant protein molecules inside live cells. Science 281 (1998) 269-272
6. Hainfeld J.F., Liu W., Halsey C.M.R., Freimuth P., and Powell R.D. Ni-NTA-Gold clusters target His-tagged proteins. J Struct Biol 127 (1999) 185-198
7. Hanaichi T., Sato T., Iwamoto T., Malavasi-Yamashiro J., Hoshino K., and Mizuno N. A stable lead by modification of Sato's method. J Electron Microsc (Tokyo) 35 (1986) 304-306
8. Kugler S., Meyn L., Holzmuller H., Gerhardt E., Isenmann S., Schulz J.B., et al. Neuron-specific expression of therapeutic proteins: evaluation of different cellular promoters in recombinant adenoviral vectors. Mol Cell Neurosci 17 (2001) 78-96
9. Martone M.E., Zhang Y., Simpliciano V.M., Carragher B.O., and Ellisman M.H. Three-dimensional visualization of the smooth endoplasmic reticulum in Purkinje cell dendrites. J Neurosci 13 (1993) 4636-4646
10. Norcott J.P., Solari R., and Cutler D.F. Targeting of p-selectin to two regulated secretory organelles in PC12 cells. J Cell Biol 134 (1996) 1229-1240
11. Pelham H.R. Recycling of proteins between the endoplasmic reticulum and Golgi complex. Curr Opin Cell Biol 3 (1991) 585-591
12. Popov V., Medvedev M.I., Davies H.A., and Stewart M.G. Mitochondria form a filamentous reticular network in hippocampal dendrites but are present as discrete bodies in axons: a three-dimensional ultrastructural study. J Comp Neurol 492 (2005) 50-65
13. Roessel van P., and Brand A.H. Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins. Nat Cell Biol Rev (2002) E15-E20
14. Schikorski T., and Stevens C.F. Morphological correlates of functionally defined synaptic vesicle populations. Nat Neurosci 4 (2001) 391-395
15. Trachtenberg J.T., Chen B.E., Knott G.W., Feng G., Sanes J.R., Welker E., et al. Long-term imaging of experience-dependent synaptic plasticity in adult cortex. Nature 420 (2002) 788-794
16. Veitch N.C. Horseradish peroxidase: a modern view of a classic enzyme. Phytochemistry 65 (2004) 249-259