One-step purification of histidine-tagged profilin with high purity and yield by using metal precipitation

Biotechnology and Applied Biochemistry

Volumn 47, Issue 3-4, 2007, Pages 185-189

  Sankian, Mojtaba ^a   Yousefi, Mitra ^a   Pazouki, Nazanin ^a   Varasteh, Abdolreza ^a  

^a MASHHAD UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Histidine tag (His tag); Imidazole; Metal ion; Metal affinity precipitation; One step purification; Profilin

Indexed keywords

ADDITIVES; BACTERIA; CELLS; METAL IONS; PRECIPITATION (CHEMICAL); PURIFICATION;

HISTIDINE TAG (HIS TAG); METAL-AFFINITY PRECIPITATION; ONE-STEP PURIFICATION;

PROTEINS;

BIOPOLYMER; HISTIDINE; PROFILIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL LYSATE; LYSIS; MELON; NUCLEOTIDE SEQUENCE; PRECIPITATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

AFFINITY LABELS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; HUMANS; METALS; PROFILINS; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); CUCUMIS; CUCUMIS MELO;

EID: 34547655966     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20060214     Document Type: Article

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