Distinct labelling of fusion events in rat lactotrophs by FM 1-43 and FM 4-64 is associated with conformational differences

Acta Physiologica

Volumn 191, Issue 1, 2007, Pages 35-42

  Stenovec, M ^a,b   Solmajer T ^c,d   Perdih, A ^c   Vardjan, N ^a,b   Kreft, M ^a,b   Zorec, R ^a,b  

^a Celica Biomedical Sciences Center   (Slovenia)

^b UNIVERSITY OF LJUBLJANA   (Slovenia)

^c NATIONAL INSTITUTE OF CHEMISTRY   (Slovenia)

^d Drug Discovery   (Slovenia)

Author keywords

Conformational analysis; Exocytosis; FM 1 43; FM 4 64; Lactotrophs; Monte Carlo; Vesicles

Indexed keywords

FLUORESCENT DYE; FM 1 43; FM 4 64; PROLACTIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL CULTURE; CELL LABELING; CELL MEMBRANE; CHEMICAL STRUCTURE; CONFOCAL MICROSCOPY; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; FLUORESCENCE; MEMBRANE VESICLE; MONTE CARLO METHOD; NONHUMAN; PRIORITY JOURNAL; RAT; STAINING;

ANIMALS; CELL MEMBRANE; CELLS, CULTURED; FLUORESCENT DYES; LACTOTROPHS; MALE; MEMBRANE FUSION; MICROSCOPY, CONFOCAL; MOLECULAR CONFORMATION; POTASSIUM; PROTEIN BINDING; PYRIDINIUM COMPOUNDS; QUATERNARY AMMONIUM COMPOUNDS; RATS; RATS, WISTAR; SECRETORY VESICLES; STAINING AND LABELING;

EID: 34547654318     PISSN: 17481708     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1748-1716.2007.01716.x     Document Type: Article

References (28)

1. Allinger, N.L., Yuh, Y.H. Lii, J.J. 1989. Molecular mechanics. The MM3 force field for hydrocarbons I. J Am Chem Soc 111, 8551 9556.
2. Angleson, J.K., Cochilla, A.J., Kilic, G., Nussinovitch, I. Betz, W.J. 1999. Regulation of dense core release from neuroendocrine cells revealed by imaging single exocytic events. Nat Neurosci 2, 440 446.
3. Ben-Tabou, S., Keller, E. Nussinovitch, I. 1994. Mechanosensitivity of voltage-gated calcium currents in rat anterior pituitary cells. J Physiol 476, 29 39.
4. Betz, W.J. Bewick, G.S. 1992a. Optical analysis of synaptic vesicle recycling at the frog neuromuscular junction. Science 255, 200 203.
5. Betz, W.J. Bewick, G.S. 1993. Optical monitoring of transmitter release and synaptic vesicle recycling at the frog neuromuscular junction. J Physiol 460, 287 309.
6. Betz, W.J., Mao, F. Bewick, G.S. 1992b. Activity-dependent fluorescent staining and destaining of living vertebrate motor nerve terminals. J Neurosci 12, 363 375.
7. Betz, W.J., Bewick, G.S. Ridge, R.M. 1992c. Intracellular movements of fluorescently labeled synaptic vesicles in frog motor nerve terminals during nerve stimulation. Neuron 9, 805 813.
8. Betz, W.J, Mao, F. Smith, C.B. 1996. Imaging exocytosis and endocytosis. Curr Opin Neurobiol 6, 365 371.
9. Bolte, S., Talbot, C., Boutte, Y., Catrice, O., Read, N.D. Satiat-Jeunemaitre, B. 2004. FM-dyes as experimental probes for dissecting vesicle trafficking in living plant cells. J Microsc 214, 159 173.
10. Brumback, A.C., Lieber, J.L., Angleson, J.K. Betz, W.J. 2004. Using FM1-43 to study neuropeptide granule dynamics and exocytosis. Methods 33, 287 294.
11. Buckton G., Beezer, A.E., Denyer, S.P., Russell, S.J. 1991, Observations on the biopharmaceutical importance of chain length in chemically related compounds. Int J Pharm 73, 1 7.
12. Chang, G., Guida, W.C. Still, W.C. 1989. An internal coordinate Monte Carlo method for searching conformational space. J Am Chem Soc 111, 4379 4386.
13. Cochilla, A.J, Angleson, J.K. Betz, W.J. 1999. Monitoring secretory membrane with FM1-43 fluorescence. Annu Rev Neurosci 22, 1 10.
14. Cochilla, A.J., Angleson, J.K. Betz, W.J. 2000. Differential regulation of granule-to-granule and granule-to-plasma membrane fusion during secretion from rat pituitary lactotrophs. J Cell Biol 150, 839 848.
15. Fischer-Parton, S., Parton, R.M., Hickey, P.C, Dijksterhuis, J., Atkinson, H.A. Read, N.D. 2000. Confocal microscopy of FM4-64 as a tool for analysing endocytosis and vesicle trafficking in living fungal hyphae. J Microsc 198, 246 259.
16. Leach, A.R. 1991. A survey of methods for searching the conformational space of small and medium -sized molecules. In : K. Lipkowitz D. Boyd (eds) Reviews in Computational Chemistry, pp. 1 55, Vol 2. VCH Publishers, New York.
17. Li, Z. Scheraga, H.A. 1987. Monte Carlo minimization approach to the multiple minima problem in protein folding. Proc Natl Acad Sci USA 84, 6611 6615.
18. Metropolis, N., Rosenbluth, W., Rosenbluth, M.N, Teller, A.H Teller, E. 1953. Equation of state calculations by fast computing machines. J Chem Phys 21, 1087 1092.
19. Miesenböck, G., De Angelis, D.A. Rothman, J.E. 1998. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 394, 192 195.
20. Richards, D.A., Bai, J. Chapman, E.R. 2005. Two modes of exocytosis at hippocampal synapses revealed by rate of FM1-43 efflux from individual vesicles. J Cell Biol 168, 929 939.
21. Rizzoli, S.O., Richards, D.A. Betz, W.J. 2003. Monitoring synaptic vesicle recycling in frog motor nerve terminals with FM dyes. J Neurocytol 32, 539 549.
22. Schote, U. Seelig, J. 1998. Interaction of the neuronal marker dye FM1-43 with lipid membranes. Thermodynamics and lipid ordering. Biochim Biophys Acta 1415, 135 146.
23. Stenovec, M., Kreft, M., Poberaj, I., Betz, W.J. Zorec, R. 2004. Slow spontaneous secretion from single large dense-core vesicles monitored in neuroendocrine cells. FASEB J 18, 1270 1272.
24. Stenovec, M., Poberaj, I., Kreft, M. Zorec, R. 2005. Concentration- dependent staining of lactotroph vesicles by FM 4-64. Biophys J 88, 2607 2613.
25. Taraska, J.W. Almers, W. 2004. Bilayers merge even when exocytosis is transient. Proc Natl Acad Sci USA 101, 8780 8785.
26. Vardjan, N., Stenovec, M., Jorgačevski, J., Kreft, M. Zorec, R. 2007. Subnanometer fusion pores in spontaneous exocytosis of peptidergic vesicles. J Neurosci 21, 4737 4746.
27. Vida, T.A. Emr, S.D. 1995. A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast. J Cell Biol 128, 779 792.
28. Zenisek, D., Steyer, J.A., Feldman, M.E. Almers, W. 2002. A membrane marker leaves synaptic vesicles in milliseconds after exocytosis in retinal bipolar cells. Neuron 35, 1085 1097.