Aequorin luminescence-based assay for 5-hydroxytryptamine (serotonin) type 3 receptor characterization

Analytical Biochemistry

Volumn 368, Issue 2, 2007, Pages 185-192

  Walstab, Jutta ^a   Combrink, Sandra ^a   Brüss, Michael ^a   Göthert, Manfred ^a   Niesler, Beate ^b   Bönisch, Heinz ^a  

^a UNIVERSITY OF BONN   (Germany)

^b UNIVERSITY OF HEIDELBERG   (Germany)

Author keywords

5 HT3 receptor; Aequorin luminescence; Ca2+ influx; HEK293 cells; Ligand gated ion channels

Indexed keywords

CELL CULTURE; ELECTROPHYSIOLOGY; HYDRAULIC GATES; LIGANDS; LUMINESCENCE;

5-HT3 RECEPTOR; ELECTRO-PHYSIOLOGICAL METHODS; FUNCTIONAL CHARACTERIZATION; HEK293 CELLS; HIGH THROUGHPUT SCREENING; HUMAN EMBRYONIC KIDNEYS; LIGAND-GATED ION CHANNELS; PHARMACOLOGICAL CHARACTERIZATION;

CELLS;

AEQUORIN; SEROTONIN 2A RECEPTOR; SEROTONIN 2B RECEPTOR; SEROTONIN 3 AGONIST; SEROTONIN 3 ANTAGONIST; SEROTONIN 3 RECEPTOR;

ANALYTIC METHOD; ARTICLE; BIOLUMINESCENCE; CELL SUSPENSION; COMPARATIVE STUDY; CONTROLLED STUDY; EMBRYO; FLUORESCENCE; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SIGNAL NOISE RATIO;

EID: 34547564432     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.06.007     Document Type: Article

References (32)

1. 3 receptors are membrane ion channels. Nature 339 (1989) 706-709
2. 3 receptor. Trends Pharmacol. Sci. 26 (2005) 587-594
3. 3B subunit is a major determinant of serotonin-receptor function. Nature 397 (1999) 359-363
4. 3B receptor subunit. J. Biol. Chem. 274 (1999) 30799-30810
5. 3 serotonin receptor-like genes HTR3C, HTR3D, and HTR3E. Gene 310 (2003) 101-111
6. A). J. Physiol. 507 (1998) 653-665
7. 2+ permeability of cloned and native 5-hydroxytryptamine type 3 receptors. Mol. Pharmacol. 46 (1994) 1120-1128
8. 2+- and membrane potential-sensitive dyes: advantages and potential problems. J. Neurosci. Methods 149 (2005) 172-177
9. Shimomura O., and Johnson F.H. Regeneration of the photoprotein aequorin. Nature 256 (1975) 236-238
10. Shimomura O. Luminescence of aequorin is triggered by the binding of two calcium ions. Biochem. Biophys. Res. Commun. 211 (1995) 359-363
11. An S., Bleu T., and Zheng Y. Transduction of intracellular calcium signals through G protein-mediated activation of phospholipase C by recombinant sphingosine 1-phosphate receptors. Mol. Pharmacol. 55 (1999) 787-794
12. Sheu Y.A., Kricka L.J., and Pritchett D.B. Measurement of intracellular calcium using bioluminescent aequorin expressed in human cells. Anal. Biochem. 209 (1993) 343-347
13. Ungrin M.D., Singh L.M., Stocco R., Sas D.E., and Abramovitz M. An automated aequorin luminescence-based functional calcium assay for G-protein-coupled receptors. Anal. Biochem. 272 (1999) 34-42
14. 2 nicotinic acetylcholine receptors stably expressed in HEK293T cells. J. Neurochem. 91 (2004) 1138-1150
15. Wallis D.I., and North R.A. The action of 5-hydroxytryptamine on single neurones of the rabbit superior cervical ganglion. Neuropharmacology 17 (1978) 1023-1028
16. Fozard J.R. Neuronal 5-HT receptors in the periphery. Neuropharmacology 23 (1984) 1473-1486
17. Azami J., Fozard J.R., Round A.A., and Wallis D.I. The depolarizing action of 5-hydroxytryptamine on rabbit vagal primary afferent and sympathetic neurones and its selective blockade by MDL 72222. Naunyn Schmiedebergs Arch. Pharmacol. 328 (1985) 423-429
18. Round A., and Wallis D.I. The depolarizing action of 5-hydroxytryptamine on rabbit vagal afferent and sympathetic neurones in vitro and its selective blockade by ICS 205-930. Br. J. Pharmacol. 88 (1986) 485-494
19. 3 receptors. Trends Pharmacol. Sci. 13 (1992) 391-397
20. 50) of an enzymatic reaction. Biochem. Pharmacol. 22 (1973) 3099-3108
21. 14C]-guanidinium. Br. J. Pharmacol. 108 (1993) 436-442
22. 3 receptors. Br. J. Pharmacol. 142 (2004) 501-508
23. c): a critical evaluation. J. Biol. Chem. 270 (1995) 9896-9903
24. Williams M.E., Brust P.F., Feldman D.H., Patthi S., Simerson S., Maroufi A., McCue A.F., Velicelebi G., Ellis S.B., and Harpold M.M. Structure and functional expression of an omega-conotoxin-sensitive human N-type calcium channel. Science 257 (1992) 389-395
25. 3A receptors in outside-out patches of HEK 293 cells: basic properties and barbiturate effects. Naunyn Schmiedebergs Arch. Pharmacol. 362 (2000) 255-265
26. Knight M.R., Read N.D., Campbell A.K., and Trewavas A.J. Imaging calcium dynamics in living plants using semi-synthetic recombinant aequorins. J. Cell Biol. 121 (1993) 83-90
27. Shimomura O., Kishi Y., and Inouye S. The relative rate of aequorin regeneration from apoaequorin and coelenterazine analogues. Biochem. J. 296 (1993) 549-551
28. 3A receptor. Pharmacogenetics 14 (2004) 165-172
29. Spier A.D., and Lummis S.C. The role of tryptophan residues in the 5-hydroxytryptamine-3 receptor ligand binding domain. J. Biol. Chem. 275 (2000) 5620-5625
30. 3 receptors and emesis. Pharmacol. Rep. 58 (2006) 253-255
31. 3A receptor isoforms. Naunyn Schmiedebergs Arch. Pharmacol. 362 (2000) 392-401
32. 3 receptor. Biophys. J. 84 (2003) 1720-1733